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Gradient polyacrylamide gel electrophoresis

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4%−15% gradient polyacrylamide gel electrophoresis is a laboratory equipment used for the separation and analysis of proteins based on their molecular weight. It consists of a polyacrylamide gel with a gradient concentration ranging from 4% to 15%, which allows for the effective separation of a wide range of protein sizes.

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Lab products found in correlation

Chemidoc xrs system, by Bio-Rad (2 mentions) Bradford method, by Bio-Rad (2 mentions) Ripa buffer, by Merck Group (1 mentions) Anti cd63, by Santa Cruz Biotechnology (1 mentions) Radioimmunoprecipitation assay buffer, by Merck Group (1 mentions)

Close spelling variants of this product

4–15% gradient Tris–HCl polyacrylamide gels for electrophoresis fractionation Polyacrylamide gel electrophoresis 4–20% gradient polyacrylamide gel 4–15% gradient gel electrophoresis Polyacrylamide gel 4–20% gradient gels

2 protocols using gradient polyacrylamide gel electrophoresis

1

EV Protein Characterization by Western Blot

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EVs were lysed with RIPA buffer (Sigma Aldrich). Protein content was measured by Bradford method (Bio-Rad). EVs lysates (30μg) were separated by 4%−15% gradient polyacrylamide gel electrophoresis (Bio-Rad) and then immunoblotted with antibody anti-CD63 (Santa Cruz). The protein bands were visualized with an enhanced chemiluminescence (ECL) detection kit and ChemiDoc™ XRS-System (Bio-Rad).
Cheng Y., Pereira M., Raukar N., Reagan J.L., Queseneberry M., Goldberg L., Borgovan T., LaFrance WC J.r., Dooner M., Deregibus M., Camussi G., Ramratnam B, & Quesenberry P. (2019). Potential biomarkers to detect traumatic brain injury by the profiling of salivary extracellular vesicles. Journal of Cellular Physiology, 234(8), 14377-14388.
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2

Characterization of Extracellular Vesicles

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EVs were lysed with radioimmunoprecipitation assay buffer (Sigma‐Aldrich, St. Louis, MO). Protein content was measured by the Bradford method (Bio‐Rad). EVs lysates (30 μg) were separated by 4–15% gradient polyacrylamide gel electrophoresis (Bio‐Rad, Hercules, CA) and then immunoblotted with antibody anti‐CD63 (Santa Cruz, biotechnology, Santa Cruz, CA). The protein bands were visualized with an enhanced chemiluminescence detection kit and ChemiDoc™ XRS‐System (Bio‐Rad, Hercules, CA).
Cheng Y., Pereira M., Raukar N., Reagan J.L., Queseneberry M., Goldberg L., Borgovan T., LaFrance WC J.r., Dooner M., Deregibus M., Camussi G., Ramratnam B, & Quesenberry P. (2019). Potential biomarkers to detect traumatic brain injury by the profiling of salivary extracellular vesicles. Journal of Cellular Physiology, 234(8), 14377-14388.
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