Log In Sign up
The largest database of trusted experimental protocols

Secondary antibodies coupled to irdye800 fluorophore

Manufactured by Rockland Immunochemicals
Visit Supplier
Sourced in United States

Secondary antibodies coupled to IRDye800 fluorophore. These antibodies are designed to detect and bind to primary antibodies, amplifying the signal for improved detection and visualization in various immunoassay applications.

Automatically generated - may contain errors

Lab products found in correlation

Phospho ampkα, by Cell Signaling Technology (1 mentions) Control rabbit igg, by Santa Cruz Biotechnology (1 mentions) Ikkα β, by Abcam (1 mentions) 17 dmag, by Merck Group (1 mentions) Monoclonal antibodies against, by Cell Signaling Technology (1 mentions) P p70s6k, by Abcam (1 mentions) P ikkα β, by Abcam (1 mentions) Odyssey infrared imaging system, by Rockland Immunochemicals (1 mentions) Ampkα, by Cell Signaling Technology (1 mentions) Lamin b1, by Abcam (1 mentions) β actin, by Abcam (1 mentions) P iκbα, by Abcam (1 mentions) Hif 1α, by Abcam (1 mentions) P mtor, by Abcam (1 mentions) Gefitinib, by Selleck Chemicals (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) 6 thioguanine 6 tg, by Merck Group (1 mentions) Erlotinib hcl, by Selleck Chemicals (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) Nh4cl, by Merck Group (1 mentions)

2 protocols using secondary antibodies coupled to irdye800 fluorophore

1

Signaling Pathway Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies against Hsp90β, TAK1, p-TAK1 (phospho-T187), TAB-1, ERK1/2, p-ERK1/2 (ERK1: phospho-Y204, ERK2: phospho-Y187), JNK1/2, p-JNK1/2 (phospho-T183/Y185), p38, p-p38 (phospho-T180/Y182), IκBα, p-IκBα (phospho-S32/S36), NF-κB p65, Bcl-2, cytochrome c, p-Bcl-2 (phospho-S87), p-p70S6 K (phospho-T389), p-mTOR (phospho-S2448), IKKα/β, p-IKKα/β (phsopho-S176/S177), HIF-1α, Lamin B1 (internal standard in nuclear protein fractions), β-actin (internal standard in cytosolic protein fractions) were from Abcam. Monoclonal antibodies against MKK3/6, p-MKK3/6 (MKK3: phospho-S189, MKK6: phospho-S207), AMPKα and phospho-AMPKα (phosphor-T172) were from Cell Signaling Technology and R&D Systems, respectively. Control rabbit IgG was from Santa Cruz Biotechnology. Horseradish peroxidase-conjugated secondary antibodies were obtained from Calbiochem. Secondary antibodies coupled to IRDye800 fluorophore for use with the Odyssey Infrared Imaging System were purchased from Rockland. Hsp90 inhibitor, the water-soluble 17-DMAG, was purchased from Sigma Aldrich.
Yan S.H., Zhao N.W., Jiang W.M., Wang X.T., Zhang S.Q., Zhu X.X., Zhang C.B., Gao Y.H., Gao F., Liu F.M, & Fang Z.Y. (2016). Hsp90β is involved in the development of high salt-diet-induced nephropathy via interaction with various signalling proteins. Open Biology, 6(4), 150159.
+ Open protocol
+ Expand
2

Antibody Validation for Cell Signaling Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbit monoclonal antibodies against HSP27, HSP90, FEN1, phosphor-H2AX (Ser139), EGFR, phospho-EGFR (Y1068), Akt, phospho-Akt (Ser473) and mouse monoclonal antibody against ubiquitin (Ub) were purchased from Cell Signaling Technology (Beverly, MA, USA). Mouse monoclonal antibody against HSP70 was obtained from Stressgen Bioreagents (Victoria, BC, Canada). Pol β polyclonal antibody was obtained from Abcam (Cambridge, United Kingdom). Rabbit polyclonal antibodies against GAPDH and Flag-tag were obtained from Bioworld Biotechnology (Minneapolis, MN, USA). Rabbit polyclonal antibody against HA-tag was obtained from Santa Cruz Biotechnology (Wembley, Middlesex, UK). Secondary antibodies coupled to IRDye800 fluorophore were purchased from Rockland (Gilbertsville, PA, USA). Erlotinib HCL (CAS: 183319-69-9) and Gefitinib (CAS: 184475-35-2) were from Selleckchem (Houston, TX, USA). Methyl methanesulfonate (MMS), MG132, NH4Cl, 6-Thioguanine (6-TG) and adenosine triphosphate (ATP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Cycloheximide (CHX) was obtained from Calbiochem (San Diego, Calif., USA). Hydrogen peroxide (H2O2) was obtained from Merck (Darmstadt, Germany).
Cao X., Zhou Y., Sun H., Xu M., Bi X., Zhao Z., Shen B., Wan F., Hong Z., Lan L., Luo L., Guo Z, & Yin Z. (2018). EGFR-TKI-induced HSP70 degradation and BER suppression facilitate the occurrence of the EGFR T790M resistant mutation in lung cancer cells. Cancer letters, 424, 84-96.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!