PBMC were isolated from peripheral blood by Ficoll gradient and frozen for batched analysis. We designed six multicolor flow cytometry panels to quantify 60 T cell subsets along with two B cell subsets, and calculated the CD4+/CD8+ T cell ratio (Supplementary Table 1 ). The following fluorochrome-conjugated anti-human antibodies were used from BD Biosciences (San Jose, CA): CD3-FITC, CD3-PerCP-Cy5.5, CD4-APC, CD4-APC-Cy7, CD8-BV510, CD45RO-FITC, CD45RA-APC, CD45RA-APC-Cy7, CCR4-PE, CD27-PE, CD28-BV421, CD138-BV421, CCR6-BV421, CXCR3-PE, CCR7-A700, IL-17-BV786, IFN-γ-PE-Cy7, iso IgG1k-FITC, iso IgG1k-PE-Cy7, iso IgG2bk-APC, iso IgG1k-APC-Cy7, and iso IgG1k-BV510; from Biolegend (San Diego, CA): CD127-FITC, CD27-APC, CD57-PerCp-Cy5.5, CD19-BV510, PD-1-APC-Cy7, CXCR5-FITC, and TNFα-FITC; from eBioscience (San Diego, CA): CD4-PE-Cy7, and IL-2-PE; from Miltenyi Biotec (San Diego, CA): CD25-APC and KLRG1-PE; from Beckman Coulter (Brea, CA): CD38-PE-Cy7.
Cd57 percp cy5
Manufactured by Thermo Fisher Scientific
Sourced in United States
CD57-PerCp-Cy5.5 is a fluorescent-labeled antibody used for the detection and analysis of CD57-positive cells in flow cytometry applications. It contains the CD57 antigen conjugated to the PerCp-Cy5.5 fluorescent dye.
Automatically generated - may contain errors
Lab products found in correlation
Pd 1 apc cy7, by Thermo Fisher Scientific (2 mentions)
Cd8 bv510, by BioLegend (1 mentions)
Cd28 bv421, by BioLegend (1 mentions)
Cd4 apc, by BioLegend (1 mentions)
Cd45ro fitc, by BioLegend (1 mentions)
Cd45ra apc, by BioLegend (1 mentions)
Cd45ra apc cy7, by BioLegend (1 mentions)
Ccr4 pe, by BioLegend (1 mentions)
Cd138 bv421, by BioLegend (1 mentions)
Ccr6 bv421, by BioLegend (1 mentions)
Il 17 bv786, by BioLegend (1 mentions)
Ifn γ pe cy7, by BioLegend (1 mentions)
Tnfα fitc, by Thermo Fisher Scientific (1 mentions)
Il 2 pe, by Miltenyi Biotec (1 mentions)
Cd25 apc, by Beckman Coulter (1 mentions)
Cd38 pe cy7, by Beckman Coulter (1 mentions)
Cxcr3 pe, by BioLegend (1 mentions)
Cd27 pe, by BioLegend (1 mentions)
Cd127 fitc, by Thermo Fisher Scientific (1 mentions)
Cd3 percp cy5, by BD (1 mentions)
2 protocols using cd57 percp cy5
1
Multicolor Flow Cytometry for T and B Cell Subsets
2
Flow Cytometry Immunophenotyping Protocol
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Flow cytometry was performed at the LIM/60, Laboratorio de Imunologia Clinica e
Alergia, Universidade de Sao Paulo, Sao Paulo State, Brazil. Briefly, frozen
PBMC were thawed in a 37 ºC water bath and cells were counted (1x106/
tube) and incubated with the following conjugated monoclonal antibodies: (I)
Invitrogen (Carlsbad, California, USA): CD3 phycoerythrin (PE)-Texas Red; (II)
BD Biosciences Pharmingen (San Diego, California, USA): CD8 Brilliant violet
(BV) 605, CD38 allophycocyanin (APC); (III) Biolegend (San Diego, CA, USA):
HLA-DR Alexa fluor 700, CD127 PE-Cy5, PD-1 APC-Cy7, CD28 BV 785, CD57
PerCP-Cy5.5; (IV) eBioscience (Carlsbad, CA, USA): TIGIT. Live dead amine aqua
dye (Invitrogen, Carlsbad, CA, USA) was used to exclude dead cells. The analyses
were performed using FlowJo software (version 10, TreeStar Inc., Ashland, USA),
and a representative strategy used to analyze the T cell populations is shown in
Figure 2 .
Alergia, Universidade de Sao Paulo, Sao Paulo State, Brazil. Briefly, frozen
PBMC were thawed in a 37 ºC water bath and cells were counted (1x106/
tube) and incubated with the following conjugated monoclonal antibodies: (I)
Invitrogen (Carlsbad, California, USA): CD3 phycoerythrin (PE)-Texas Red; (II)
BD Biosciences Pharmingen (San Diego, California, USA): CD8 Brilliant violet
(BV) 605, CD38 allophycocyanin (APC); (III) Biolegend (San Diego, CA, USA):
HLA-DR Alexa fluor 700, CD127 PE-Cy5, PD-1 APC-Cy7, CD28 BV 785, CD57
PerCP-Cy5.5; (IV) eBioscience (Carlsbad, CA, USA): TIGIT. Live dead amine aqua
dye (Invitrogen, Carlsbad, CA, USA) was used to exclude dead cells. The analyses
were performed using FlowJo software (version 10, TreeStar Inc., Ashland, USA),
and a representative strategy used to analyze the T cell populations is shown in
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