Tris hydrochloride acid buffer ph 7.5

Daunorubicin hydrochloride (daunomycin, DM) and the antioxidant 2-mercaptoethanol (2-ME) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Spermine tetrahydrochloride (SP) was purchased from Nacalai Tesque (Kyoto, Japan). The fluorescent cyanine dye YOYO-1 (1,10-(4,4,8,8-tetramethyl-4,8-diazaundecamethylene)bis[4-[(3-methylbenzo-1,3-oxazol-2-yl) methylidene]-l,4-dihydroquinolinium] tetraiodide) was purchased from Molecular Probes, Inc. (Eugene, OR, USA). T4 GT7 DNA (166 kbp with a contour length of 57 µm) and Tris-hydrochloride acid buffer (pH 7.5) were purchased from Nippon Gene (Tokyo, Japan). Plasmid DNA (Luciferase T7 Control DNA, 4331 bp) containing a firefly luciferase gene and a T7 RNA polymerase promoter sequence was purchased from Promega (Madison, WI, USA).

Spermidine trihydrochloride (SPD) was purchased from Nacalai Tesque (Kyoto Japan). Polyethylene Glycol 6,000 (PEG), Dextran 200,000 (DEX) and the antioxidant 2-mercaptoethanol (2-ME) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). T4 GT7 bacteriophage DNA (166 kbp with a contour length of 57 μm) and Tris-hydrochloride acid buffer (ph7.5) were purchased from Nippon Gene (Tokyo, Japan). The dimeric cyanine fluorescent dye YOYO-1 (1,10-(4,4,8,8-tetramethyl-4,8-diazaundecamethylene)bis[4-[(3-methylbenzo-1,3-oxazol-2-yl)methylidene]-l,4-dihydroquinolinium] tetraiodide) was obtained from Molecular Probes Inc. (OR, USA).