Poly l lysine coating solution

Commercially available kits for assessment of glucose, low density lipoproteins (LDL), high density lipoproteins (HDL), triglycerides (TG’s), total cholesterol (TC), and aspartate transaminase (AST) were purchased from Tulip diagnostics (P) Ltd. (Mumbai, India) and Arkray Healthcare Pvt. Ltd, (Surat, India). Streptozotocin (STZ), hematoxylin and eosin (H&E), Sirius red, poly-l-lysine coating solution, Griess reagent and primers were purchased from Sigma Aldrich (St. Louis, Missouri, United States). Anti- PKR, JNK, and β-actin antibodies and the secondary antibodies were purchased from Santa Cruz Biotechnology (CA, USA). Masson’s trichrome and TUNEL assay kits were purchased from Abcam (Milton, Cambridge, UK). iScript cDNA synthesis kit, iTaq Universal SYBR® Green Supermix and the Immun-Blot® PVDF Membrane were purchased from Bio-Rad (Hercules, CA, USA). Trizol reagent was purchased from In-vitrogen.

The following fluorescent antibodies (Abs) and materials were procured from Medi’Ray New Zealand Ltd: [conjugated Ab FITC anti-human CCR5] (Cat: 313705), [conjugated Ab Alexa Fluor 488 anti-human CD4] (Cat: 317419), purified primary antibody against CCR5 N-terminal, [unconjugated Ab 3A9] (Cat: 634501) and [conjugated Ab Alexa Fluor 488 Goat anti-mouse IgG] (Cat: 405319). Cell fixation buffer: 4% Paraformaldehyde (PFA) (Cat: 420801), plastic coverslips (No.: BAH446900000), glass slides (No.: S8400-1PAK), Poly-L-lysine coating solution (No.: P4707), and anti-fade mounting medium fluoro-shield (No.: F6937) were procured from Sigma-Aldrich. In addition, purified primary antibody against NPC proteins, [unconjugated anti-Nup 153] (Cat: 906201), [conjugated Ab Alexa Fluor 555 Goat anti-mouse IgG] (Cat: 405324) and -diamidino-2-phenylindole (DAPI) (Cat:422801) were procured from Medi’Ray, along with 3 glass chambers-3cm, forceps for mounting coverslips, and clear finger nail polish to seal coverslips on slide. Phosphate buffer saline (1X PBS) and Serums, HCl acid, Ethanol and Methanol were obtained through courtesy of the Massey Human Nutrition Lab, along with all other apparatus such as beakers, Parafilm, polypropylene tubes, 1 mL pipette, 100 pipette, pipette, pipette and their pipette tips.