Mccoy s 5a medium

Manufactured by Macgene

Sourced in China, Israel

McCoy's 5A medium is a cell culture medium formulated to support the growth and maintenance of various cell types. It provides the necessary nutrients, vitamins, and other components required for cell proliferation and survival in vitro.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using mccoy s 5a medium

Colorectal Cancer Cell Lines and Spheroids

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human colorectal cancer cell line HT29 was purchased from Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai Institute of Cell Biology, Chinese Academy of Sciences). The human colorectal cancer cell line HCT116, DLD1 and HCT8 were granted by Department of Immunology of the Fourth Military Medical University. HT29 and HCT116 cell lines were maintained in McCOY's 5A medium (MACGENE)supplemented with 10% fetal calf serum (Gibco), DLD1 and HCT8 cell lines were maintained in RPMI 1640 medium (HyClone) supplemented with 10% fetal calf serum(Gibco). These cell lines were incubated in a humidified atmosphere with 5% CO₂ at 37°C. To generate spheroid bodies, single cells were seeded on ultralow attachment plates (Corning) at a density of 1,000 cells/mL. Cells were maintained in DMEM/F12 (Gibco) supplemented with B-27 (Gibco), basic fibroblast growth factor (PeproTech), epidermal growth factor (PeproTech), 0.4% bovine serum albumin (Sigma), and 4 mg/mL Insulin (Sigma) and were incubated in a humidified atmosphere with 5% CO₂ at 37°C. The average number of spheroids (with a size>50μm) was calculated 5-7 days after seeding under microscope.

Liu S., Zhang C., Zhang K., Gao Y., Wang Z., Li X., Cheng G., Wang S., Xue X., Li W., Zhang W., Zhang Y., Xing X., Li M, & Hao Q. (2017). FOXP3 inhibits cancer stem cell self-renewal via transcriptional repression of COX2 in colorectal cancer cells. Oncotarget, 8(27), 44694-44704.

+ Open protocol

+ Expand

Breast Cancer Cell Lines and Conditioned Media

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Three types of human breast carcinoma cell line, including MCF7, MDA-MB-231 (M231) and SK-BR-3, were used in this study. Primary human normal fibroblasts (NF) were isolated as described previously and used as a control [28 (link)]. MCF7 and NF were cultured in Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (Hyclone, Logan, UT, USA). M231 was cultured in L-15 (Macgene, Beijing, China). SK-BR-3 was cultured in McCoy’s 5A medium (Macgene). All of the cell culture media were supplemented with 10% fetal bovine serum (Hyclone), 100 U/mL penicillin, and 100 U/mL streptomycin. All cells were cultured at 37°C with 5% CO₂ and 95% relative humidity. CM was collected from serum-free medium cultured on each cell line for 48 h, and centrifuged at 2000 rpm for 15 min to delete cell debris for exosome capture. All of the samples were stored at −80°C until use.

Fang S., Tian H., Li X., Jin D., Li X., Kong J., Yang C., Yang X., Lu Y., Luo Y., Lin B., Niu W, & Liu T. (2017). Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification. PLoS ONE, 12(4), e0175050.

+ Open protocol

+ Expand

Cell Line Cultivation and Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ishikawa and HEC‐1A cell lines were purchased from ZhongQiaoXinZhou Biotechnology Co., Ltd. (Shanghai, China). Ishikawa cells were cultured in RPMI 1640 medium (BI, Israel), and HEC‐1A cells were cultured in McCoy’s 5A medium (Macgene, Beijing, China). Both media were supplemented with 10% fetal bovine serum (FBS), and cells were cultured at 4 °C in a humidified atmosphere containing 5% CO₂. All cell lines were mycoplasma‐free, and short tandem repeats (STRs) were confirmed.

Kong D., Hou Y., Li W., Ma X, & Jiang J. (2021). LncRNA‐ZXF1 stabilizes P21 expression in endometrioid endometrial carcinoma by inhibiting ubiquitination‐mediated degradation and regulating the miR‐378a‐3p/PCDHA3 axis. Molecular Oncology, 16(3), 813-829.

+ Open protocol

+ Expand

Endometrial Cancer Cell Lines Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human endometrial cancer cell lines Ishikawa and HEC-1A were purchased from the ZhongQiaoXinZhou Biotechnology Co., Ltd. (Shanghai, China). The human endometrial cancer cell line RL95-2 was purchased from the Shanghai Cell Bank (Shanghai, China). Ishikawa, HEC-1A and RL95-2 cells were cultured in RPMI 1640 medium (BI, Israel), McCoys 5A medium (Macgene, Beijing, China) and DMEM/F12 medium (BI, Israel) supplemented with 10% foetal bovine serum (FBS; HyClone), streptomycin (100g/mL) and penicillin (100 U/mL) (Thermo Scientific, Waltham, MA, USA). Cells were cultured at 37C in the presence of a humidified atmosphere with 5% CO₂. All cell lines were free of mycoplasma and were verified by short tandem repeats (STRs). LPCAT1-specific small interfering RNA (siRNA) and a control scrambled siRNA used as a negative control (NC) were designed and synthesized by GenePharma. The nucleotide sequences of the siRNAs were as follows: si-LPCAT1 (sense 5-CCAUGACGAUGUCCUCCAUTT-3, antisense 5-AUGGAGGACAUCGUCAUGGTT-3) and si-NC (sense 5-UUCUCCGAACGUGUCACGUTT-3, antisense 5-ACGUGACACGUUCGGAGAATT-3). Cells were transfected using Lipofectamine 3000 Reagent (Invitrogen, CA, USA) according to the manufacturers protocol at 50% confluency.

Zhao T., Zhang Y., Ma X., Wei L., Hou Y., Sun R, & Jiang J. (2021). Elevated expression of LPCAT1 predicts a poor prognosis and is correlated with the tumour microenvironment in endometrial cancer. Cancer Cell International, 21, 269.

+ Open protocol

+ Expand

Cellular Signaling Pathway Investigation

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293T, MCF7 and HeLa cells were cultured in Dulbecco’s modified Eagle’s medium (Macgene). HCT116 cells were cultured in McCoy’s 5A medium (Macgene). hTERT-RPE1 cells were cultured in DMEM/F12 medium (Gibco). Penicillin, streptomycin and 10% foetal bovine serum were added to all culture media unless otherwise noted. D4476 was purchased from Tocris Bioscience. IC261 was purchased from Selleckchem. Rabbit anti-Dvl2 and rabbit anti-PP2A/C antibodies were purchased from Cell Signaling Technology. Mouse anti-Dvl2 (10B5) and mouse anti-Myc antibodies were purchased from Santa Cruz Biotechnology. Rabbit anti-Dvl2 (phospho S143) and rabbit anti-γ-tubulin antibodies were purchased from Abcam. Mouse anti-PP5 antibody (for Western blotting) was purchased from BD Transduction Laboratories. Rabbit anti-PP5 antibody (for immunofluorescence) was purchased from Bethyl. Mouse anti-FLAG, mouse anti-acetylated tubulin and mouse anti-γ-tubulin antibodies were purchased from Sigma-Aldrich. Mouse anti-β-actin antibody was purchased from Sungene.

Xie J., Han M., Zhang M., Deng H, & Wu W. (2018). PP5 (PPP5C) is a phosphatase of Dvl2. Scientific Reports, 8, 2715.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!