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Mouse cxcl1 kc duoset

Manufactured by R&D Systems
Sourced in United States

The Mouse CXCL1/KC DuoSet is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse CXCL1/KC concentrations in cell culture supernatants, cell lysates, serum, plasma, and other biological fluids.

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4 protocols using mouse cxcl1 kc duoset

1

Cytokine Quantification ELISA Protocols

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Protein concentrations were determined using the Mouse CXCL1/KC Quantikine ELISA Kit (#MKC00B) or the Mouse CXCL1/KC DuoSet (#DY453), the Mouse CXCL2/MIP-2 DuoSet (#DY452), the Mouse IL-6 DuoSet (#DY406) (R&D Systems) or the Mouse BD OptEIA IL-10 kit (BD Biosciences, #555252). The ELISAs were performed according to the manufacturer`s instructions.
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2

Cytokine Quantification ELISA Protocols

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Protein concentrations were determined using the Mouse CXCL1/KC Quantikine ELISA Kit (#MKC00B) or the Mouse CXCL1/KC DuoSet (#DY453), the Mouse CXCL2/MIP-2 DuoSet (#DY452), the Mouse IL-6 DuoSet (#DY406) (R&D Systems) or the Mouse BD OptEIA IL-10 kit (BD Biosciences, #555252). The ELISAs were performed according to the manufacturer`s instructions.
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3

CXCL1 Concentration Quantification by ELISA

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KC/CXCL1 serum concentration was quantified by ELISA using mouse CXCL1/KC DuoSet (R&D Systems, Minneapolis, Minnesota, USA) according to the instructions of the manufacturer. Assay Range: 16–1000 pg/mL.
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4

Murine Lung CXCL1/KC ELISA Protocol

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For the assessment of CXCL1/KC concentrations in murine lungs ELISA assays were performed using chemokine specific ELISA kits (Mouse CXCL1/KC DuoSet, R&D Systems, Minneapolis) according to the manufacturer's instruction. Therefore, 100 mg of lung tissue were homogenised in 1 mL PBS containing anti-proteases (0.1 mM phenylmethylsulfonyl fluoride, 0.1 mM benzethonium chloride, 10 mM EDTA, 20 KI Aprotinin A) and 0.05 % Tween 20. The samples were then centrifuged for 10 min at 3000 g and the supernatant immediately used for ELISA. All samples were assayed in duplicates.
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