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Anti cypa

Manufactured by Santa Cruz Biotechnology
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Anti-CypA is a lab equipment product developed by Santa Cruz Biotechnology. It is designed to detect and quantify the presence of Cyclophilin A (CypA) in various biological samples.

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Lab products found in correlation

Anti ocn, by Abcam (1 mentions) Anti cathepsin k, by Abcam (1 mentions) Biotinylated goat anti rabbit secondary antibody, by Santa Cruz Biotechnology (1 mentions) Anti ikkα, by Cell Signaling Technology (1 mentions) Anti relb, by Cell Signaling Technology (1 mentions) Anti histone h3, by Santa Cruz Biotechnology (1 mentions) Anti phospho stat3, by Santa Cruz Biotechnology (1 mentions) Anti s100a4, by Cell Signaling Technology (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti p100 p52, by Cell Signaling Technology (1 mentions) Anti p65 d14e12, by Cell Signaling Technology (1 mentions) Anti nik, by Cell Signaling Technology (1 mentions) Anti lamin, by Cell Signaling Technology (1 mentions) Anti stat3, by Santa Cruz Biotechnology (1 mentions) Anti ha, by Santa Cruz Biotechnology (1 mentions) Anti actin, by Santa Cruz Biotechnology (1 mentions) Ribavirin, by Merck Group (1 mentions) Cyclosporin a, by Merck Group (1 mentions) Bone morphogenetic protein 4 (bmp4), by Thermo Fisher Scientific (1 mentions) Ifn β, by PBL Assay Science (1 mentions)

3 protocols using anti cypa

1

Immunohistochemical Quantification of CypA, OCN, and Cathepsin K

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Immunohistochemistry analyses were performed as previously described6 . Briefly, slides were incubated with anti-CypA (1:100; Santa Cruz, Santa Cruz, CA), anti-OCN (1:100; Abcam, Cambridge, MA) or anti-Cathepsin K (1:100, Abcam) overnight at 4°C, after blocking with 1% BSA. The next day, sections were incubated with biotinylated goat anti-rabbit secondary antibody (Santa Cruz) for 1 hour at room temperature. VECTSTAIN Elite ABC complex (Vector lab, Burlingame, CA) incubation was used for primary antibody detection. Negative control sections were incubated in PBS (phosphate-buffered saline) instead of primary antibody.
Guo M., James A.W., Kwak J.H., Shen J., Yokoyama K.K., Ting K., Soo C.B, & Chiu R.H. (2016). Cyclophilin A (CypA) Plays Dual Roles in Regulation of Bone Anabolism and Resorption. Scientific Reports, 6, 22378.
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2

Immunoblot Analysis of NF-κB Pathway

Cited 1 time
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Immunoblots were performed as previously described [46 (link)] with the following primary antibodies: anti-GAPDH (sc-32,233, Santa Cruz), anti-p100/p52 (4882 or 37,359 S, Cell Signaling), anti-p105/p50 (D4P4D, Cell Signaling), anti-lamin (4777 S, Cell Signaling) anti-p65 (D14E12, Cell Signaling), anti-RelB (10,544 S, Cell Signaling), anti-IKKα (2682 S, Cell Signaling), anti-NIK (4994 S, Cell Signaling), anti-HA (sc-7392, Santa Cruz), anti-Actin (sc-8432, Santa Cruz), anti-CYPA (sc-134,310, Santa Cruz), anti-S100A4 (13,018 S, Cell Signaling), anti-Histone H3 (sc-517,576, Santa Cruz), anti-STAT3 (sc-482 X, Santa Cruz), and anti-phospho-STAT3 (sc-8059, Santa Cruz). All immunoblots are representative of at least two separate experiments and representative images displayed.
Bernal G.M., Wu L., Voce D.J., Weichselbaum R.R, & Yamini B. (2022). p52 signaling promotes cellular senescence. Cell & Bioscience, 12, 43.
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3

Immunofluorescence and Western Blot Analysis

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The following antibodies were used for immunofluorescence staining: anti-OCT3/4 (Stemgent, Cambridge, MA), anti-SEEA4 (Stemcell Technologies, Vancouver, Canada), anti-GATA4 (Cell Signaling, Danvers, MA), anti-HNF4α (Cell Signaling), anti-AFP (Sigma-Aldrich, St. Louis, MO), anti-ALB (Cedarlane, Burlington, Canada), and anti-ORF2 (a kind gift from Suzanne U. Emerson, NIH). The following antibodies were used for Western blot (WB) analysis: anti-HCV NS5A clone 9E1018 (link), anti-CypA (Santa Cruz, Dallas, TX), and anti-actin HRP (Sigma-Aldrich). IFN-β was purchased from pBL Assay Science (Piscataway, NJ), Sofosbuvir from Acme Bioscience (Palo Alto, CA), and Cyclosporin A, Ribavirin and BX795 from Sigma-Aldrich. Activin-A, Wnt-3A and oncostatin-M were purchased from R&D Systems (Minneapolis, MN), bFGF from Life Technologies, and BMP-4, EGF and HGF were purchased from Peprotech (Rocky Hill, NJ).
Wu X., Thi V.L., Liu P., Takacs C.N., Xiang K., Andrus L., Gouttenoire J., Moradpour D, & Rice C.M. (2017). Pan-Genotype Hepatitis E Virus Replication in Stem Cell-derived Hepatocellular Systems. Gastroenterology, 154(3), 663-674.e7.
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