Mice were euthanized at indicated times pi and tissues were harvested. Corneas were digested in RPMI 1640 containing 10% fetal bovine serum (FBS), 1% antibiotic/antimicotic, and gentamicin at 37° C as previously described.28 MLN were dissociated with a 3 ml syringe-plunger head in the presence of media. Cell suspensions were passed through a 40-micron filter before staining. Single cell suspensions were labeled with the following fluorochrome-conjugated monoclonal antibodies: rat anti-mouse CD45 efluor 450, rat anti-mouse CD3e FITC, rat anti-mouse CD4 APC, rat anti-mouse CD8 PE, rat anti-mouse CD3e PE-Cy7, rat anti-mouse NK1.1 FITC, rat anti-mouse CD11b APC, rat anti-mouse Ly6G FITC, rat anti-mouse Ly6C FITC (all from eBioscience), and rat anti-mouse F4/80 APC (Abcam). Labeled cells were washed in 1% BSA in PBS and analyzed using a MACS Quant flow cytometer (Miltenybiotec).
Rat anti mouse ly6c fitc
Manufactured by Thermo Fisher Scientific
The Rat anti-mouse Ly6C FITC is a fluorescently-labeled antibody that binds to the mouse Ly6C antigen. Ly6C is a cell surface marker expressed on various cell types, including monocytes and granulocytes. This antibody can be used to identify and analyze these cell populations in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Rat anti mouse cd4 apc, by Thermo Fisher Scientific (2 mentions)
Rat anti mouse cd45 efluor 450, by Thermo Fisher Scientific (2 mentions)
Rat anti mouse cd3e fitc, by Thermo Fisher Scientific (2 mentions)
Rat anti mouse cd8 pe, by Thermo Fisher Scientific (2 mentions)
Rat anti mouse cd3e pe cy7, by Thermo Fisher Scientific (2 mentions)
Rat anti mouse nk1.1 fitc, by Thermo Fisher Scientific (2 mentions)
Rat anti mouse cd11b apc, by Thermo Fisher Scientific (2 mentions)
Rat anti mouse ly6g fitc, by Thermo Fisher Scientific (2 mentions)
Rat anti mouse f4 80 apc, by Abcam (2 mentions)
Macsquant flow cytometer, by Miltenyi Biotec (2 mentions)
2 protocols using rat anti mouse ly6c fitc
1
Murine Corneal Immune Cell Analysis
2
Murine Corneal Immune Cell Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mice were euthanized at indicated times pi and tissues were harvested. Corneas were digested in RPMI 1640 containing 10% fetal bovine serum (FBS), 1% antibiotic/antimicotic, and gentamicin at 37° C as previously described.28 MLN were dissociated with a 3 ml syringe-plunger head in the presence of media. Cell suspensions were passed through a 40-micron filter before staining. Single cell suspensions were labeled with the following fluorochrome-conjugated monoclonal antibodies: rat anti-mouse CD45 efluor 450, rat anti-mouse CD3e FITC, rat anti-mouse CD4 APC, rat anti-mouse CD8 PE, rat anti-mouse CD3e PE-Cy7, rat anti-mouse NK1.1 FITC, rat anti-mouse CD11b APC, rat anti-mouse Ly6G FITC, rat anti-mouse Ly6C FITC (all from eBioscience), and rat anti-mouse F4/80 APC (Abcam). Labeled cells were washed in 1% BSA in PBS and analyzed using a MACS Quant flow cytometer (Miltenybiotec).
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