The culture medium was Multicell RPMI 1640, 1× obtained from Wisent Inc.; Saint-Jean-Baptiste, QC, Canada (350-005-CL). Primary antibodies for Western blot analysis were as follows: mouse monoclonal anti-human CD63 (10628D; Invitrogen; Waltham, MA, USA), mouse monoclonal anti-CD81 (10630D; Invitrogen), rabbit monoclonal anti-human glycophorin A (GPA-ab129024; Abcam; Cambridge, UK), rabbit polyclonal anti-P. falciparum Pf GRP78 (BiP, MRA-1246; BEI Resources; Northern Virginia, United States), rabbit monoclonal anti-flotillin 2 (ab181988; Abcam), rabbit monoclonal anti-albumin (ab192603; Abcam), rabbit polyclonal anti-hemoglobin (PA5-97488; Invitrogen). HRP-conjugated secondary antibodies purchased from Abcam were goat anti-mouse IgG (ab6789) and goat anti-rabbit (ab97080). Nalgene 0.45 μm pore PES rapid flow vacuum filter units (165-0045) and Pierce silver stain kit (24612) were from ThermoFisher; Waltham, United States. Satorius vivacell 100 PES concentrators were purchased from Fisher Scientific (VC1042).
Ab192603
Manufactured by Abcam
Sourced in United States
Ab192603 is a lab equipment product offered by Abcam. It is a tool designed for laboratory use, but a detailed description of its core function cannot be provided in an unbiased and factual manner without potential extrapolation. As a marketing specialist, I am unable to present a concise description of this product while strictly adhering to the given guidelines.
Automatically generated - may contain errors
Lab products found in correlation
Pierce silver stain kit, by Thermo Fisher Scientific (1 mentions)
Ab181988, by Abcam (1 mentions)
Ab97080, by Abcam (1 mentions)
Ab6789, by Abcam (1 mentions)
Prism 9, by GraphPad (1 mentions)
Ab124964, by Abcam (1 mentions)
Ab133496, by Abcam (1 mentions)
Ab125212, by Abcam (1 mentions)
Ab16667, by Abcam (1 mentions)
Pp k9218 00, by R&D Systems (1 mentions)
Microscope dm 1000, by Leica (1 mentions)
Ab955, by Abcam (1 mentions)
Thp 1 cell, by American Type Culture Collection (1 mentions)
Glutaraldehyde, by Polysciences (1 mentions)
Ab23722, by Abcam (1 mentions)
Ab125145, by Abcam (1 mentions)
Ab150687, by Abcam (1 mentions)
Biosol, by National Diagnostics (1 mentions)
Ab197896, by Abcam (1 mentions)
Discovery ultra automated slide preparation system, by Roche (1 mentions)
7 protocols using ab192603
1
Proteomics Analysis of Extracellular Vesicles
2
Characterization of Liver Spheroid Model
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Liver spheroids and tissues were fixed in neutral buffered formalin for 1 day at room temperature and submitted to HistoWiz Inc. (Brooklyn, NY, USA) for paraffin processing and sectioning. Paraffin sections (4 µm) were analyzed by direct fluorescence microscopy, H&E staining and IHC. IHC antibodies included anti-HNF4α (PP-K9218-00, R&D Systems, Minneapolis, MN, USA; 1:125); anti-αSMA (ab124964, Abcam; 1:1000); anti-Ki67 (ab16667, Abcam, Cambridge, MA, USA; 1:200); anti-CK19 (ab133496, Abcam; 1:250); anti-CD68 (ab125212, Abcam; 1:1000); anti-CD31 (ab12443, Abcam; 1:1000), anti-albumin (ab192603, Abcam; 1:50) and anti-HSEC (NB110-68095, Novus Biologicals, Centennial, CO, USA; 1:250). For the co-spheroids, direct ZsG and tdT fluorescence images were taken from sections prior to IHC. HNF4A+ and Ki67+ counting was performed on three spheroids each from three independent batches of spheroids and plotted using GraphPad Prism 9.4.
3
Immunohistochemical Analysis of RGCC Expression in Colorectal Cancer
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The tumor and its paired
adjacent tissues of colorectal cancer patients were fixed with 4%
paraformaldehyde, embedded in paraffin, dewaxed, hydrated, and antigen-repaired.
After being blocked by 5% BSA (AB192603, Abcam), the tissues were
incubated by the RGCC antibody (BS-9079R, 1:200, Bioss Inc, China)
overnight at 4 °C. After being washed with phosphate-buffered
saline (PBS), the samples were incubated with the horseradish peroxidase
(HRP)-labeled broad-spectrum secondary antibody (D-3004, Shanghai
Changdao Biotechnology Co., Ltd., Shanghai, China) at 37 °C for
30 min. After dyeing with DAB (FL-6001, Shanghai Changdao Biotechnology
Co., Ltd., Shanghai, China) and hematoxylin, a Leica microscope (DM1000)
was used to collect photographs. The mean optical density (IOD) of
RGCC expression in each sample was calculated by Image-Pro Plus 6.0
analysis software. All experimental procedures were carried out in
accordance with Chinese legislation regarding medical ethics and were
approved by the Medical Ethics Committee of Zunyi Medical University
((2019) H-008).
adjacent tissues of colorectal cancer patients were fixed with 4%
paraformaldehyde, embedded in paraffin, dewaxed, hydrated, and antigen-repaired.
After being blocked by 5% BSA (AB192603, Abcam), the tissues were
incubated by the RGCC antibody (BS-9079R, 1:200, Bioss Inc, China)
overnight at 4 °C. After being washed with phosphate-buffered
saline (PBS), the samples were incubated with the horseradish peroxidase
(HRP)-labeled broad-spectrum secondary antibody (D-3004, Shanghai
Changdao Biotechnology Co., Ltd., Shanghai, China) at 37 °C for
30 min. After dyeing with DAB (FL-6001, Shanghai Changdao Biotechnology
Co., Ltd., Shanghai, China) and hematoxylin, a Leica microscope (DM1000)
was used to collect photographs. The mean optical density (IOD) of
RGCC expression in each sample was calculated by Image-Pro Plus 6.0
analysis software. All experimental procedures were carried out in
accordance with Chinese legislation regarding medical ethics and were
approved by the Medical Ethics Committee of Zunyi Medical University
((2019) H-008).
4
Histological Analysis of Mouse Liver Tissues
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Mouse liver tissues were fixed in formalin for 24 h and embedded in paraffin. Five-micron-thick sections were made from paraffin-embedded liver tissues and then stained with haematoxylin and eosin to detect histological structure and with Sirius red to detect liver fibrosis. For immunohistochemical staining, the sections were incubated with primary antibodies to ALB (ab192603, 1:300, Abcam) and CD68 (ab955, 1:200, Abcam) according to previously described protocols [16 (link)].
5
Functionalized POZ Polymers for Biomedical Applications
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Mono- and diamino-functionalized POZ, (10 kDa) were chosen as optimized polymers; these compounds were purchased from Ultroxa. 5(6)-carboxyfluorescein N-hydroxysuccinimide ester (≥80%) was purchased from Sigma. Antibodies were purchased from Abcam: anti-BSA antibody (ab192603), anti-AGE antibody (ab23722), anti-CML antibody (ab125145). Glutaraldehyde was purchased from Polysciences. Biosol was purchased from National Diagnostics. Pharmaceutical grade human serum albumin (HSA), used in the clinical grade trileaflet BHV experiments, was purchased from Octapharma. THP-1 cells, a monocyte/macrophage cell line, were purchased from ATCC. RPMI medium was purchased from Cell Culture Technologies. TNF-α ELISA was obtained from Invitrogen. Cellulose dialysis membrane was purchased from Spectrum Labs (10768-700). The Von Kossa staining kit was obtained from Abcam (ab150687). All chemicals were purchased from Sigma Aldrich, unless otherwise stated.
6
Histopathological and Immunohistochemical Analysis of Liver and Kidney Tissues
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4-µm-thick formalin-fixed paraffin-embedded liver and kidney tissue sections were used for histopathology and immunohistochemistry analyses. All stainings were performed using an autostainer (Discovery Ultra Automated Slide Preparation System, Roche, Germany) based on standard protocols (Ghallab et al. 2021b (link)). For histopathology analysis, hematoxylin and eosin staining was performed. For immunohistochemistry, the following primary antibodies were applied: anti-albumin (# ab192603, Abcam; dilution 1:500), anti-CD45 (#550539, BD Bioscience; dilution 1: 400), and anti-FSP1 (#ab197896, Abcam; dilution 1:200). Appropriate secondary antibodies were used (Hassan et al. 2022 (link)).
7
Comprehensive Histological Analysis of Liver Samples
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HSs or the recipient liver samples were stained with hematoxylin and eosin (H&E), anti-albumin antibody (ab192603, abcam), anti-apolipoprotein E antibody (ab183597, abcam), and anti-cytokeratin 18 (ab181597, abcam). Elastica-Masson staining was performed with Maeda modified resorcin-fuchsin solution (Muto Pure Chemical Co., Ltd., Tokyo, Japan), 0.75% orange G solution (Muto Pure Chemical Co., Ltd.), Masson B stain solution (Muto Pure Chemical Co., Ltd.), phosphotungstic acid (FUJIFILM Wako Pure Chemical Co., Ltd., Osaka, Japan) and aniline blue liquid (FUJIFILM Wako Pure Chemical Co., Ltd.).
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