Prostar 410 autosampler

Manufactured by Agilent Technologies

Sourced in United States

The ProStar 410 autosampler is a laboratory instrument designed for automated sample injection. It features a robotic arm for precise and consistent sample handling, allowing for unattended operation and increased throughput in analytical workflows.

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9 protocols using prostar 410 autosampler

HPLC Quantification of NFD Dissolution

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Dissolution samples were diluted with HPLC mobile phase (1:1 v/v) consisting of methanol: water: acetonitrile (36:55:9 v/v). HPLC analysis was undertaken using a Varian Prostar 230 Solvent Delivery Module, a Varian Prostar autosampler 410, and a Varian Prostar 310 UV-visible detector (Varian, Palo Alto, CA, USA). Integration of the peaks was performed with a Galaxie Chromatography Data System (Varian, CA, USA). NFD was eluted on a Thermo BDS Hypersil C18 reverse-phase column (200 × 4.6 mm, 5 μm). The mobile phase was filtered by a hydrophilic 0.45 μm filter (Millipore, Millex-LCR, Merck, Madrid, Spain), and pumped at a flow rate of 1 mL/min. The sample injection volume was 50 μL. The column temperature was kept at 25 °C, and the detector was set at 240 nm. The method used was previously validated with a detection limit of 0.12 μg/mL, while the quantification limit was 0.4 μg/mL [17 (link)].

Sánchez-Guirales S.A., Jurado N., Kara A., Lalatsa A, & Serrano D.R. (2021). Understanding Direct Powder Extrusion for Fabrication of 3D Printed Personalised Medicines: A Case Study for Nifedipine Minitablets. Pharmaceutics, 13(10), 1583.

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HPLC Analysis of Vancomycin

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Samples were analyzed using HPLC with a Varian Prostar 230 Solvent Delivery Module, Varian Prostar autosampler 410, and Varian Prostar 310 UV–visible detector (Varian, Palo Alto, CA, USA). Peak integration was analyzed using Galaxie software (version 1.9). Vancomycin was analyzed using a Nucleosil C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of 50 mM ammonium phosphate and acetonitrile (92:8, v:v) (final pH of 2.2) and was pumped at a flow rate of 0.7 mL/min. The injection volume of the sample was 40 µL. The column temperature was maintained at 25 °C, and the detector was set at 205 nm.

Sánchez-Somolinos M., Díaz-Navarro M., Benjumea A., Matas J., Vaquero J., Muñoz P., Sanz-Ruíz P, & Guembe M. (2023). In Vitro Efficacy of Dalbavancin as a Long-Acting Anti-Biofilm Agent Loaded in Bone Cement. Antibiotics, 12(9), 1445.

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Vancomycin Release Analysis via HPLC

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Samples from release studies of vancomycin were analyzed as received using an HPLC with a Varian Prostar 230 Solvent Delivery Module, Varian Prostar autosampler 410, and Varian Prostar 310 UV–visible detector (Varian, Palo Alto, CA, USA). Peak integration was analysed using the Galaxie software (version 1.9). Vancomycin was analyzed using a Nucleosil C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of 50 mM ammonium phosphate: acetonitrile (92:8, v:v), with a final pH of 2.2, which was pumped at a flow rate of 0.7 mL/min and the injection volume of the sample was 40 µL. The column temperature was maintained at 25 °C and the detector was set at 205 nm.

Sánchez-Somolinos M., Díaz-Navarro M., Benjumea A., Tormo M., Matas J., Vaquero J., Muñoz P., Sanz-Ruíz P, & Guembe M. (2022). Determination of the Elution Capacity of Dalbavancin in Bone Cements: New Alternative for the Treatment of Biofilm-Related Peri-Prosthetic Joint Infections Based on an In Vitro Study. Antibiotics, 11(10), 1300.

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Purification of SalC Assay Product

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For purification of the SalC assay product, reactions were set up as described above at a 50 mL scale in an Erlenmeyer flask. SalB-PCP loading was allowed to proceed for 12 hours, at which point approximately 1 gram of XAD7 resin and SalC (20 μM) were added to the reaction mixture and assays were allowed to proceed overnight. In the morning, resin was extracted (3×) with ethyl acetate. Organic extracts were combined and evaporated to dryness. Samples were resuspended in acetonitrile and the peak corresponding to R-17 was purified by preparative HPLC using a Phenomenex Luna C18 column (5 μm, 100 mm, 2mm i.d.), along with an Agilent Technologies system composed of a PrepStar pump, a ProStar 410 autosampler, and a ProStar UV detect (Agilent Technologies, Inc, Santa Clara, USA). The sample were eluted by a gradient from 20–100% acetonitrile over 40 min at a flow rate of 10 mL/min. The peak corresponding to R-17 was collected and dried by rotary evaporation and lyophilization. Finally, R-17 was purified using a small-scale silica column with a dichloromethane/acetonitrile solvent system. All stages of purification were monitored by LCMS analysis.

Bauman K.D., Shende V.V., Chen P.Y., Trivella D.B., Gulder T.A., Vellalath S., Romo D, & Moore B.S. (2022). Enzymatic assembly of the salinosporamide γ-lactam-β-lactone anticancer warhead. Nature chemical biology, 18(5), 538-546.

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HPLC Analysis of Biogenic Amines in Pineapple Samples

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Sample preparation and determination of BAs in Ps samples were performed according to the method of Ben‐Gigirey, Baptista de Sousa, Juan, Villa, and Barros‐Velazquez (1999). The chromatographic analyses were carried out using a Varian ProStar HPLC system (Varian Corp.) with two ProStar 210 pumps, a ProStar 410 autosampler, a ProStar 325 UV/VIS Detector, and Galaxy software (Agilent) for data processing. For the separation of amines, a Discovery ® HS C18 column (150 × 4.6 mm, 5 µm; SupelcoTM Analytical) was used. The eluents were ammonium acetate (A) and acetonitrile (B), and the elution program consisted of a gradient system with a 0.8 ml/min flow rate. The detection wavelength was set to 254 nm, the oven temperature was 40°C, and samples were injected in 20 μl aliquots. The target compounds were identified based on their retention times in comparison with their corresponding standards.

Gunasekaran Y.K., Lele V., Sakiene V., Zavistanaviciute P., Zokaityte E., Klupsaite D., Bartkevics V., Guiné R.P, & Bartkiene E. (2020). Plant‐based proteinaceous snacks: Effect of fermentation and ultrasonication on end‐product characteristics. Food Science & Nutrition, 8(9), 4746-4756.

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Determination of Biogenic Amines in Pork

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Sample preparation and determination of BA content in pork meat samples were performed according to the method of Ben-Gigirey et al. [41 (link)], with some modifications [42 (link)]. The BAs were extracted with 0.4 mol/L perchloric acid, and dansyl chloride solution in acetonitrile (10 mg/mL) was used as a derivatisation reagent. The Varian ProStar HPLC system (Varian Corp., Palo Alto, CA, USA) was composed of two ProStar 210 pumps, a ProStar 410 autosampler, a ProStar 325 UV/VIS detector and the Galaxy software (Agilent, Santa Clara, CA, USA) for data processing. For the separation of BAs, a Discovery^® HS C18 column (150 × 4.6 mm, 5 µm; SupelcoTM Analytical, Bellefonte, PA, USA) was used. The BAs were identified based on their retention times in comparison to their corresponding standards.

Badaras S., Klupsaite D., Ruzauskas M., Gruzauskas R., Zokaityte E., Starkute V., Mockus E., Klementaviciute J., Cernauskas D., Dauksiene A., Vadopalas L, & Bartkiene E. (2022). Influence of Sugar Beet Pulp Supplementation on Pigs’ Health and Production Quality. Animals : an Open Access Journal from MDPI, 12(16), 2041.

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Purification of SalC Assay Product

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LC-MS/MS Analysis of Test Compounds

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The LC–MS/MS analysis was carried out using a Varian 1200L triple quadrupole system (Palo Alto, CA, USA) equipped with two Prostar 210 pumps, a Prostar 410 auto sampler, and an electrospray source (ESI) operating in the positive ion mode. The ion sources and ion optics parameters were optimized and 1 µg/mL working solution of the test compounds was injected via syringe pump at 10 µL/min. Raw data were collected and processed by Varian Workstation Version 6.8 software.

Crocetti L., Bartolucci G., Cilibrizzi A., Giovannoni M.P., Guerrini G., Iacovone A., Menicatti M., Schepetkin I.A., Khlebnikov A.I., Quinn M.T, & Vergelli C. (2017). Synthesis and analytical characterization of new thiazol-2-(3H)-ones as human neutrophil elastase (HNE) inhibitors. Chemistry Central Journal, 11, 127.

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Quantification of Phytochemicals in A. integrifolia Micro-shoots

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High-performance liquid chromatography was performed for the quantification of phytochemicals accumulated in micro-shoot cultures of A. integrifolia. Galaxie version 1.9.3.2 software with Varian HPLC system (Varian Prostar 230 pump, Varian Prostar 410 autosampler and Varian Prostar 335 Photodiode Array Detector) was used to separate the components. Reading was measured at 320 nm. Two HPLC grade solvents i.e., HCOOH/ H₂O, pH = 2.1 (A) and CH₃OH (B) were used in the mobile phase with a flow rate 1 ml/min. Composition of mobile phase varied according to nonlinear gradient i.e., 8% B (0 min), 12% B (11 min), 30% B (17 min), 33% B (28 min), 100% B (30– 35 min), 8% B (36 min). Ten min of equilibration time after each run was applied. Phytochemicals were quantified based on retention times and UV spectra compared with authentic standards.

Ullah M.A., Gul F.Z., Khan T., Bajwa M.N., Drouet S., Tungmunnithum D., Giglioli-Guivarc’h N., Liu C., Hano C, & Abbasi B.H. (2021). Differential induction of antioxidant and anti-inflammatory phytochemicals in agitated micro-shoot cultures of Ajuga integrifolia Buch. Ham. ex D.Don with biotic elicitors. AMB Express, 11, 137.

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