Polypropylene microtubes

Manufactured by Sarstedt

Sourced in Germany, United Kingdom

Polypropylene microtubes are small, single-use containers made of durable polypropylene plastic. They are designed for a wide range of laboratory applications, including sample storage, preparation, and transportation.

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Lab products found in correlation

Eno 20 nitric oxide analyzer, by Eicom (1 mentions) Centrifuge 5810r, by Eppendorf (1 mentions) Falcon tube, by Sarstedt (1 mentions)

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Microtubes

6 protocols using polypropylene microtubes

Timed 24-hour Urine Collection Protocol

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Complete 24-h urine collection was conducted at specified times of the day on two different days separated by an interval of approximately 1 week. Sampling was performed during weekdays. In the morning of the starting day, upon rising, participants were instructed to discharge the first void of the day, recording the date and time as the starting point for the 24-h urine sampling. The participants were instructed to urinate at six fixed times (09.30, 12.00, 14.30, 17.30, 22:00 hours and first void the next morning). They were instructed to collect each void at the specific time in a separate bottle, recording the time of each void. If they needed to urinate in between the fixed times, they used the next bottle and then filled it again at the specified time point. On the next morning, they collected the first void of the day, representing the overnight urine sample. Participants were instructed to store their urine samples in a refrigerator and return all samples on the day they completed the collection.
Spot urine samples were collected in 1000 ml high-density polyethylene bottles with polypropylene screw caps (Bibby Sterilin Ltd) using no preservatives. Total volume and collection times were recorded for each urine sample. All urine samples were aliquoted into 2 ml polypropylene micro tubes (Sarstedt) and frozen within 8 h (–80°C).

Eriksson J., Barregard L., Sallsten G., Berlinger B., Weinbruch S., Manousou S., Ellingsen D.G, & Nyström H.F. (2023). Urinary iodine excretion and optimal time point for sampling when estimating 24-h urinary iodine. The British Journal of Nutrition, 130(8), 1289-1297.

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Cryopreserving Opsonized Zymozyan A EVs

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EVs induced by opsonized Zymozyan A were prepared and labelled for flow cytometric analysis as described below. Then they were suspended in HBSS and stored at different temperatures (+20°C, +4°C, −20°C, −80°C) for different periods (1 day, 7 days, 28 days). By snap-freezing, liquid nitrogen was used to freeze the samples. Samples were stored in 1.5 mL polypropylene microtubes (Sarstedt, Nümbrecht, Germany). Samples were thawed at room temperature or in 37°C water bath in case of a snap-thawed sample.

Lőrincz Á.M., Timár C.I., Marosvári K.A., Veres D.S., Otrokocsi L., Kittel Á, & Ligeti E. (2014). Effect of storage on physical and functional properties of extracellular vesicles derived from neutrophilic granulocytes. Journal of Extracellular Vesicles, 3, 10.3402/jev.v3.25465.

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Plasma Nitrite/Nitrate Analysis Protocol

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After 10 minutes of supine rest (~ 1 hour after the participant consumed the juice), venous blood samples were drawn into 4 ml lithium heparin tubes and centrifuged at 4,000 rpm at 20°C for 3 minutes within 1 minute of collection. Plasma was transferred in 400 μl volumes to sterile 500 μl Sarstedt polypropylene microtubes containing no additives and frozen at −70°C for later analysis. Nitrite (NO₂⁻) and nitrate were measured as described previously (42 (link)) using an ENO-20 nitric oxide analyzer (EICOM, San Diego, CA USA).

Eggebeen J., Kim-Shapiro D.B., Haykowsky M., Morgan T.M., Basu S., Brukaker P., Rejeski J, & Kitzman D.W. (2016). One Week of Daily Dosing with Beetroot Juice Improves Submaximal Endurance and Blood Pressure in Older Patients with Heart Failure and Preserved Ejection Fraction. JACC. Heart failure, 4(6), 428-437.

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Maternal and Infant Plasma Sampling

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A sample of maternal plasma, milk and infant plasma were collected from each mother–infant pair (n = 30 pairs) within 24 h, and collected from all pairs between April and August 2013.
Blood was drawn by venepuncture from fasted individuals. Maternal fasted blood sampling times ranged from 09:03 to 11:45 h, and infant times ranged from 09:00 to 11:50 h. Infants were fed 0.20–2.65 h before venepuncture. Lithium Hepaparin plasma monovette tubes were placed on ice immediately and centrifuged (4 °C, 1800 × g for 20 min (HNR: Mistral 6000 Centrifuge, Sanyo Gallenkamp PLC., Leicester, UK; MRC Keneba: Centrifuge 5810R, Eppendorf, Stevenage, UK) within 1 h of collection. Centrifugation was repeated for slightly haemolysed samples. Lithium Heparin plasma aliquots were transferred to polypropylene microtubes (1.5 mL, Sarstedt AG & Co, Leicester, UK), and stored at − 80 to − 70 °C in the MRC Keneba laboratory until analysed (Keneba-SOP-4045; HNR-SOP-0367).

Furse S., Billing G., Snowden S.G., Smith J., Goldberg G, & Koulman A. (2019). Relationship between the lipid composition of maternal plasma and infant plasma through breast milk. Metabolomics, 15(10), 129.

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Biomarker Profiling of Neuroinflammation

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Samples of CSF on polypropylene tubes, blood on dry tube are rapidly transported, centrifuged (10 min at 1300 g for serum samples and 5 min at 300 g for CSF samples), aliquoted within 2 h in polypropylene microtubes (Sarstedt 72.692.005) and frozen at − 80 °C until analysis. Nf-L levels were measured with the R-PLEX Human Neurofilament L Antibody Set from MesoScale Discovery (MSD) (F217X, MSD, USA) according to the manufacturer's instructions, Pro-inflammatory cytokines (Il-1β, IL-6, IL-8, IL-17a, IFN-γ, TNF-α), anti-inflammatory cytokine (IL-10), IL-2, chemokines (IP-10, MCP-1, Eotaxin) and vascular markers: VCAM-1, ICAM-1, VEGF) were quantified using the V-PLEX Neuroinflammation Panel 1 Human Kit (K15210D, MSD, USA) according to manufacturer’s instructions.

Brodovitch A., Boucraut J., Delmont E., Parlanti A., Grapperon A.M., Attarian S, & Verschueren A. (2021). Combination of serum and CSF neurofilament-light and neuroinflammatory biomarkers to evaluate ALS. Scientific Reports, 11, 703.

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Serum Sex Steroids Stability Study

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This method comparison study was conducted by staff and the advisory group on endocrinology at Equalis AB, Uppsala, Sweden in August 2020. The test material consisted of five pooled serum samples from children 4-18 years of age, without any additives. In each material, about 30 leftover samples, either from prepubertal children (samples A and E), boys in early puberty (sample B), girls in early-mid puberty (sample C) or boys in early-mid puberty (sample D), were pooled together.
The sera were left-over samples from hormone determinations at Tillväxtlaboratoriet, Gothenburg, Sweden. The deidentified samples were stored at -20 °C for a maximum of three months and sent frozen in Falcon tubes (Sarstedt) to Equalis AB, Uppsala, Sweden. After being divided into 1 mL aliquots, samples were sent to participants in polypropylene micro tubes (Sarstedt) at 21 °C (±1 °C), to participants. Stability tests were conducted in previous studies. Evaluation showed that serum sex steroids were not affected by storage at 21 °C (±1 °C) for three days, in +2 to +8 °C for up to three weeks, long-term storage in -20 °C or repeated thaw/freeze cycles [12, 13] .

Ankarberg-Lindgren C., Becker C., Svala E, & Ryberg H. (2024). Methodological considerations in determining sex steroids in children: comparison of conventional immunoassays with liquid chromatography-tandem mass spectrometry. Clinical chemistry and laboratory medicine, 62(1).

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