Ta dsc q 2000 instrument

The thermal properties of cutin obtained from the different wastes were evaluated by thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). TGA tests were performed, in triplicate, with a TGA/SDTA 851 Mettler Toledo thermal analyzer (Schwarzenbach, Switzerland). Approximately 6 mg of each sample was heated from 25 to 700 °C at 10 °C/min under nitrogen atmosphere (flow rate 50 mL/min). DSC tests were carried out to determine the glass transition temperature (T_g) by using a TA DSC Q-2000 instrument (New Castle, DE, USA) under nitrogen atmosphere (flow rate 50 mL/min). Then, 4 mg of samples were initially submitted to −90 °C in isothermal mode for 3 min. The temperature program that followed consisted of a first heating from −90 to 150 °C, then cooling to −90 °C and a further second heating to 150 °C, all of the stages at 10 °C/min heating/cooling rate. Three replicates of each sample were performed.

The thermal stability of OE was studied, in triplicate, by thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). TGA tests were carried out using a TGA/SDTA 851 Mettler Toledo (Mettler Toledo, Schwarzenbach, Switzerland) thermal analyzer. A total of 5.0 ± 0.1 mg of OE was heated from 30 to 850 °C at 5 °C min⁻¹ under nitrogen atmosphere (50 mL min⁻¹). Two thermal parameters were determined: the initial degradation temperature, T_ini (°C), calculated at 5% of weight loss, and the temperature of maximum degradation, T_max (°C), corresponding to the maximum decomposition rate. Dried OE after 24 h at 60 °C was also analyzed by TGA.
DSC tests were conducted with a TA DSC Q-2000 instrument (TA Instruments, New Castle, DE, USA) under nitrogen atmosphere (50 mL min⁻¹). In total, 8.0 ± 0.1 mg of OE was introduced in aluminum pans and they were cooled from 30 °C to −80 °C (3 min hold), heated to 250 °C (3 min hold), cooled to −80 °C (3 min hold) and finally heated to 250 °C (3 min hold), with all steps at 10 °C min⁻¹ [50 (link)]. Calorimetric curves were analyzed in the second heating scan with the Universal Analysis TM Software (TA Instruments, New Castle, DE, USA) to obtain crystallization and melting parameters.

Thermogravimetric analysis (TGA) tests were performed with a TGA/SDTA 851 Mettler Toledo thermal analyzer (Mettler-Toledo S.p.A., Milan, Italy). Approximately 3 mg of samples were heated from 25 °C to 700 °C at a heating rate of 10 °C/min under a nitrogen atmosphere (flow rate 50 mL/min).
Differential scanning calorimetry (DSC) tests were carried out to determine glass transition temperature (T_g) values in all developed films by using a TA DSC Q-2000 instrument (New Castle, DE, USA) under a nitrogen atmosphere (flow rate 50 mL/min). Samples (3 mg) were initially submitted to −90 °C in isothermal mode for 3 min. The temperature program followed consisted of a first heating from −90 to 200 °C, then cooling to −90 °C, and a further second heating to 200 °C, all these stages at 10 °C/min heating/cooling rate. Three replicates of each sample were performed.
Data processing was performed using OriginPro 2015 software (OriginLab Corp., Northampton, MA, USA).