Amplex red
Amplex Red is a fluorogenic substrate used for the detection and quantification of hydrogen peroxide (H2O2) and other peroxides. It functions by producing a red-fluorescent product upon oxidation, allowing for sensitive and specific measurement of peroxide levels.
Lab products found in correlation
11 protocols using amplex red
Redox Cycling Compound Assay
Membrane Lipid Dynamics Analysis
Cluster analysis was performed using VutaraSRX cluster analysis tool over multiple images. GraphPad Prism was used to determine significance for raft size; CTxB (n=1,907), CTxB+mβCD (n=344), PIP2 control (n=3,076), PIP2+mβCD (n=778). Cell differentiation assay was performed single blind on images taken from multiple dishes (n=4 for each condition). All numbers are reported as mean±s.e.m. unless otherwise noted. As all samples were found to have a normal distribution, Student's t-test was used to determine significance with resultant P-values as reported.
Hemin-Assisted G-Quadruplex RNAzyme Assay
Quantifying Lipid Metabolism Markers
Adipocyte Lipolysis Assay Protocol
Adipocyte Lipolysis Assay Protocol
Measuring Cellular Oxidative Stress
Zinc Acetate-Mediated Synthesis
dihydrate (J.T. Baker); pure ethanol (Koptec); ethanolamine (Alfa
Aesar); Al (RD Mathis); aurochloric acid trihydrate (Beantown Chemical);
Amplex Red (Cayman Chemical); horseradish peroxidase (Amresco/VWR);
sodium phosphate monobasic and dibasic (Mallinckrodt Chemical); Decon
90 (Electron Microscopy Sciences); isopropanol (Fisher Chemicals);
hydrogen peroxide, 35% w/w (BDH/VWR); and ammonium hydroxide 28–30%
w/w (BDH/VWR).
H2O2 Quantification in Fibroblasts
Quantifying Hydrogen Peroxide Levels in Neuronal Cells
For determination of H2O2 accumulating in the medium during the treatment period, 50 µl conditioned cell culture supernatant of each well were transferred to a new 96-well plate and supplemented with 5 µM Amplex Red (Cayman Chemical, Ann Arbor, USA) and 0.01 U/ml HRP (Thermo Fischer Scientific, Schwerte, Germany). A potential interference between the added agents and the HRP-catalyzed reaction between Amplex Red and H2O2 was analyzed by repeating this experiment in a cell-free system utilizing freshly prepared DMEM/0.1% FCS + phospholipids (10 µM) or solvent in the presence of supplemented H2O2 (0.000007%).
For measuring the H2O2 freshly released by pretreated cells, the supernatant was removed and 50 µl of Amplex Red reaction mixture (5 μM Amplex Red and 0.01 U/ml HRP in phenol red-free DMEM/0.1% FCS) was added to each well.
The fluorescence signal of resorufin was determined at an excitation wavelength of 530 ± 17 nm and an emission wavelength of 590 ± 17 nm for 16 min (120 s (seconds) intervals) at 37 °C under light exclusion in a Safire2 Fluorometer (Tecan, Crailsheim, Germany). The increase of fluorescence over time was calculated for each well and used for further data analysis.
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