Gpr65 ires cre

Manufactured by Jackson ImmunoResearch

Gpr65-ires-Cre is a genetic construct that expresses the Cre recombinase enzyme under the control of the Gpr65 promoter. It is used in research applications to target and manipulate Gpr65-expressing cells.

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4 protocols using gpr65 ires cre

Transgenic Mouse Strains for Neuroscience

Cited 2 times

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Wild-type C57BL/6J (000664), Glp1r-ires-Cre (029283), Gpr65-ires-Cre (029282), Npy2r-ires-Cre (029285), P2ry1-ires-Cre (029284), Agtr1a-Cre (030553), Calb2-ires-Cre (010774), Nts-Cre (017525), Vglut2-ires-Cre (016963), Sst-ires-Cre (013044), Twist2-Cre (008712), Vip-ires-Cre (010908), Piezo2-GFP-ires-Cre (027719), Trpv1-Cre (017769), Pvalb-Cre (017320), Vglut1-ires2-Cre (023527), Chat-ires-Cre (031661), lox-ChR2 (024109), lox-tdTomato (007914), and Snap25-2A-GCaMP6s-D (025111) were from the Jackson Laboratory. Drd2-Cre (032108-UCD) mice were from the Mutant Mouse Resource and Research Center (MMRRC). lox-L10-GFP mice were described before^{11 (link),12 (link)}.

Zhao Q., Yu C.D., Wang R., Xu Q.J., Dai Pra R., Zhang L, & Chang R.B. (2022). A multidimensional coding architecture of the vagal interoceptive system. Nature, 603(7903), 878-884.

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Mouse Genetic Tools for Neuroscience

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All animal procedures followed the ethical guidelines outlined in the NIH Guide for the Care and Use of Laboratory Animals, and all protocols were approved by the Institutional Animal Care and Use Committee at Harvard Medical School. Animals were maintained under constant temperature (23 ± 1 °C) and relative humidity (46 ± 5%) with a 12-h light–dark cycle. Mice used in this study were from both sexes and of a mixed genetic background. Glp1r-ires-cre (029283), Gpr65-ires-cre (029282) and Crhr2-ires-cre (033728) mice were previously made in the laboratory and are now available at the Jackson Laboratory. Slc17a6-ires-cre (Vglut2-ires-cre, 028863), Slc32a1-ires-cre (Vgat-ires-cre, 028862), Rosa26-lsl-Gfp-L10a (024750), Tac1-ires-cre (021877), Pdyn-ires-cre (027958), Penk-ires-cre (025112), Cartpt-ires-cre (028533), Sst-ires-cre (013044), Th-cre (021877) and Gcg-cre (030542) mice were purchased from the Jackson Laboratory.

Ran C., Boettcher J.C., Kaye J.A., Gallori C.E, & Liberles S.D. (2022). A brainstem map for visceral sensations. Nature, 609(7926), 320-326.

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Mouse Cre Line Characterization

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Phox2b-Cre (Scott et al., 2011 (link)) (JAX# 016223), Wnt1-Cre (Chai et al., 2000 (link)) (JAX# 009107), Trpv1-ires-Cre (Cavanaugh et al., 2011 (link)) (JAX# 017769), Tac1-ires-Cre (Harris et al., 2014 (link)) (JAX# 021877), Sst-ires-Cre (Taniguchi et al., 2011 (link)) (JAX# 013044), Vglut3-ires-Cre (Tasic et al., 2018 (link)) (JAX# 028534), Glp1r-ires-Cre (Williams et al., 2016 (link)) (JAX# 029283), Gpr65-ires-Cre (Williams et al., 2016 (link)) (JAX# 029282) were previously described and purchased from Jackson Laboratories.

Borgmann D., Ciglieri E., Biglari N., Brandt C., Cremer A.L., Backes H., Tittgemeyer M., Wunderlich F.T., Brüning J.C, & Fenselau H. (2021). Gut-brain communication by distinct sensory neurons differently controls feeding and glucose metabolism. Cell Metabolism, 33(7), 1466-1482.e7.

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Transgenic Mouse Lines for Neural Tracing

Cited 2 times

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Wild-type C57BL/6J (000664), Glp1r-ires-Cre (029283), Gpr65-ires-Cre (029282), Npy2r-ires-Cre (029285), P2ry1-ires-Cre (029284), Agtr1a-Cre (030553), Calb2-ires-Cre (010774), Nts-Cre (017525), Vglut2-ires-Cre (016963), Sst-ires-Cre (013044), Twist2-Cre (008712), Vip-ires-Cre (010908), Piezo2-GFP-ires-Cre (027719), Trpv1-Cre (017769), Pvalb-Cre (017320), Vglut1-ires2-Cre (023527), Chat-ires-Cre (031661), lox-ChR2 (024109), lox-tdTomato (007914), and Snap25–2A-GCaMP6s-D (025111) were from the Jackson Laboratory. Drd2-Cre (032108-UCD) mice were from the Mutant Mouse Resource and Research Center (MMRRC). lox-L10-GFP mice were described before^{11 (link),12 (link)}.

Zhao Q., Yu C.D., Wang R., Xu Q.J., Dai Pra R., Zhang L, & Chang R.B. (2022). A multidimensional coding architecture of the vagal interoceptive system. Nature, 603(7903), 878-884.

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