Lyophilized papain (~30,000 USP units/mg) was purchased from Himedia, India. Sodium acetate (anhydrous, ≥99%), trichloroacetic acid (≥99%), and 2,4,6-Tris(2-pyridyl)-s-triazine (≥98%) were obtained from the Sigma-Aldrich group (Schnelldorf, Germany). Citric acid (99%), potassium sulfate (≥99%), copper sulfate (≥99%) sulfuric acid (≥99%), phenolphthalein (≥98%), methanol (≥98%), glucose (≥99%), phenol (≥99%), and sodium hydroxide (≥99%) were purchased from Reanal (Budapest, Hungary). Ultrasil P3-11 was purchased from Ecolab-Hygiene Kft (Budapest, Hungary). Ferric chloride (≥99%), sulfuric acid (≥99.9%), amyl alcohol (≥99.9%), ascorbic acid (99.7%), bacteriological agar powder, and soybean casein digestive medium were procured from Merck (Darmstadt, Germany). Acrylamide (≥99%), sodium-dodecyl sulfate (≥99%), ammonium persulfate (≥99%), Tetramethylethylenediamine (≥99%), tris(hydroxymethyl)aminomethane (≥99%), glycine (≥99%), ethyl alcohol (≥99%), coomassie blue stain (≥99%), acetic acid (≥99%), isopropanol (≥99%), glycerol (≥99%), 2-βmercaptoethanol (≥99%), and bromophenol blue (≥99%) were purchased from BIO-RAD (BIO-RAD, USA). Milli-Q ultrapure deionized water (18.2 MΩ·cm; Merck-Millipore, Molsheim, France) was used throughout the experiment.
Amyl alcohol
Manufactured by Merck Group
Sourced in Germany
Amyl alcohol is a colorless, flammable liquid organic compound with the chemical formula C5H12O. It is commonly used as a solvent and in the production of other chemicals.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Merck Group (3 mentions)
Sulfuric acid, by Merck Group (2 mentions)
Chloroform, by Merck Group (2 mentions)
Mayer s reagent, by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Ethyl acetate, by Merck Group (2 mentions)
Ferric chloride, by Merck Group (1 mentions)
Acrylamide, by Bio-Rad (1 mentions)
Sodium dodecyl sulfate (sds), by Bio-Rad (1 mentions)
Ammonium persulfate, by Bio-Rad (1 mentions)
Tetramethylethylenediamine, by Bio-Rad (1 mentions)
Bromophenol blue, by Bio-Rad (1 mentions)
Glycerol, by Bio-Rad (1 mentions)
Glycine, by Bio-Rad (1 mentions)
Sodium hydroxide (naoh), by Reanal (1 mentions)
Coomassie blue stain, by Bio-Rad (1 mentions)
Acetic acid, by Bio-Rad (1 mentions)
Isopropanol, by Bio-Rad (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Tris hydroxymethyl aminomethane, by Bio-Rad (1 mentions)
9 protocols using amyl alcohol
1
Quantitative Papain Activity Assay
2
Phytochemical Screening of Extracts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methanol, ethanol, double distilled water, iron (III) chloride, gelatin, ammonia, chloroform, hydrochloric acid, Mayer’s reagent, Dragendorff’s reagent, magnesium, amyl alcohol, ether, vanillin-sulfuric acid reagent, sodium hydroxide, and Liebermann–Burchard reagent were purchased from Merck (Kenilworth, NJ, USA), without further purifications.
3
Phytochemical and Enzyme Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FeCl3, Lead acetate, Amyl alcohol, Dragendroff’s reagent, Wagner’s reagent, Mayer’s reagent, Borntrager’s reagent, Salkowski’s reagent, and Acetic acid were procured from Merck, Germany. In addition, enzymes including α-glucosidase, α-amylase, Urease, Tyrosinase, Lipoxygenase, Butyrylcholinesterase, and Acetylcholinesterase were purchased from Sigma Aldrich, USA.
4
Phytochemical Screening and Bioassay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Na-CMC, distilled water, methanol 98%, Dragendorff, reagent Mayer, iron (III) chloride, reagent Molish, lead (II) acetate, hydrochloric acid, Liebermann–Burchard, chloroform-isopronolol, amyl alcohol, magnesium and Whatman filter paper were analytical grade and provided from Merck. Paracetamol and silymarin were purchased from the local pharmacy. The kits for the assay of total bilirubin, direct bilirubin, albumin, and GGT were provided from Sigma Aldrich.
5
Comprehensive Analytical Procedures for Food Aroma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methanol (p.a.) and sodium sulfate (p.a.) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The following reference compounds were purchased from Merck Group (Shanghai, China): dichloromethane (99.8%), N,N-dimethylmethanamine (p.a.), and chloroform (p.a.) 2-methyl-1-propanal, diacetyl, hexanal, 2-pentyl furan, amyl alcohol, 2-methyl pyrazine, octanal, acetone alcohol, 2,6-dimethyl pyrazine, 2-ethyl pyrazine, 2,3-dimethyl pyrazine, dimethy trisulfide, nonanal, 2-ethyl-6-methyl pyrazine, 2,3,5-trimethyl pyrazine, trans-2-octenal, 1-octen-3-one, 2,3-dimethyl-5-ethylpyrazine, 2,3,5,6-tetramethyl pyrazine, benzaldehyde, (E)-2-nonenal, 2-propyl-pyridine, gamma-butyrolactone, furan-2-ylmethanol, 2-hexylthiophene, 2-undecenal, (E,E)-2,4-decadienal, and (R)-(-)-pantolactone. The alkane mixes C5-C40 were purchased from Anpel (Shanghai, China). The purity of these and the aforementioned compounds was not less than 97%.
6
Colorimetric Indole Quantification Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Kovacs assay protocol was modified from the method of Darkoh et al. [34 (link)] in order to detect indole in the range of 10–40 μM as well as the normal sensitivity range of 50–500 μM. 200 μL of culture was centrifuged at 11,337 x g (Eppendorf, Minispin Plus, Hamburg, Germany) for 2 min. 100 μL of the supernatant was mixed with 150 μL of Kovacs reagent (5 g p-dimetylaminobenzaldehyde, Sigma, Belgium; 75 mL amyl alcohol, Sigma, Germany; 25 mL 37% w/w HCl, Fisher Scientific, Loughborough, UK) and incubated at room temperature for 5 min. The absorbance of the reaction mix was measured at 530 nm using a SpectraMax 190 microplate reader (Molecular Devices, Sunnyvale, USA). The concentration of indole was calculated using a standard curve generated with indole (Sigma, USA) standards dissolved in ethanol.
7
Electrochemical Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Phenolphthalein (C20H14O4, 98%), methanol (CH3OH, 99.8%), ethanol (C2H5OH, 99.5%), acetone
(CH3COCH3, 99.5%), 4-nitrophthalonitrile (C8H3N3O2, 99.0%), potassium
carbonate (K2CO3, 99.0%), cobalt(II) chloride
hexahydrate (CoCl2•6H2O, 98%), amyl alcohol
(C5H12O, 99%), DBU (C9H16N2, 98%), n-hexane (C6H14, 95%), ethyl acetate (C4H8O2, 99.8%), dimethyl formamide (DMF: C3H7NO,
99.8%), dimethyl sulfoxide (DMSO: C2H6OS, 99.9%),
tetrabutylammonium perchlorate [TBAP: (C4H9)4N(ClO4), 99.0%], hydrochloric acid (HCl, 37%),
sulfuric acid (H2SO4, 97%), isopropyl alcohol
(IPA, 99.0%), and potassium ferricyanide (K4[Fe(CN)6], 97%) were purchased from Sigma-Aldrich, Pt/C (20%) was
purchased from Thermo Fisher Scientific, and MWCNTs were purchased
from AkzoNobel. Other chemical reagents used to prepare solutions
were obtained from Merck. Double-distilled water was used throughout
the experiments.
(CH3COCH3, 99.5%), 4-nitrophthalonitrile (C8H3N3O2, 99.0%), potassium
carbonate (K2CO3, 99.0%), cobalt(II) chloride
hexahydrate (CoCl2•6H2O, 98%), amyl alcohol
(C5H12O, 99%), DBU (C9H16N2, 98%), n-hexane (C6H14, 95%), ethyl acetate (C4H8O2, 99.8%), dimethyl formamide (DMF: C3H7NO,
99.8%), dimethyl sulfoxide (DMSO: C2H6OS, 99.9%),
tetrabutylammonium perchlorate [TBAP: (C4H9)4N(ClO4), 99.0%], hydrochloric acid (HCl, 37%),
sulfuric acid (H2SO4, 97%), isopropyl alcohol
(IPA, 99.0%), and potassium ferricyanide (K4[Fe(CN)6], 97%) were purchased from Sigma-Aldrich, Pt/C (20%) was
purchased from Thermo Fisher Scientific, and MWCNTs were purchased
from AkzoNobel. Other chemical reagents used to prepare solutions
were obtained from Merck. Double-distilled water was used throughout
the experiments.
8
Phenolic Compounds Extraction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All solvents and reagents used in this study, Folin–Ciocalteu’s phenol reagent, sodium carbonate (Na2CO3), 2,2-Diphenyl-1-picrylhydrazyl (DPPH), ethanol (≥96%), sodium hydroxide (NaOH), sulfuric acid (H2SO4), amyl alcohol, Fehling′s A solution (CuSO4), and Fehling′s B solution (KOH and Na-K tartrate), were of analytical grade and purchased from Sigma–Aldrich (Milan, Italy). HPLC grade water, acetonitrile, and methanol were obtained from VWR (Milan, Italy). Pure luteolin, luteolin 7-O-glucoside, and apigenin-7-O-glucoside were provided by Extrasynthese (Lyon, France). Rutin (quercetin 3-O-rutinoside), apigenin, caffeic acid, chlorogenic acid, p-coumaric acid, ferulic acid, hydroxytyrosol, oleuropein, and 3,4-dihydroxyphenilacetic acid (DOPAC) were obtained from Fluka (Sigma-Aldrich, Milan, Italy).
9
Whey Bioconversion by Yeast and Enzymes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whey produced during Swiss cheese production was obtained from the Key Laboratory of Dairy Science, Northeast Agricultural University, Harbin, China, and all whey samples were stored at -20°C until processing. Sweet whey was made using liquid bovine rennet (containing 95% chymosin and 5% pepsin) obtained from New England Cheesemaking Supply Company, as earlier described by Timón et al. (2014) (link). Industrial alcohol active dry yeast S. cerevisiae (containing rhizopus, α-amylase, glucoamylase, phytase) was purchased from Angel Yeast Co. Ltd. Lactase (10,000 acid lactase unit/g, 37°C, pH 6) was bought from the Nanning Pangbo Bioengineering Co. Ltd. We purchased α-dlactose monohydrate, d-galactose, and d-(+)-glucose compounds from Shanghai Gold Wheat Biological Technology Company, prepared from commercially available standards, purity grade ≥98%. Ethyl acetate, acetaldehyde, acetic acid, methanol, n-butanol, 1-propanol, 2-butanol, amyl alcohol, isobutyl alcohol, isoamyl alcohol, and 4-nanonol (GC standard purity) were purchased from Sigma-Aldrich as internal standards.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!