We measured bacterial production and extracellular enzyme activity in August 2016. Bacterial production was estimated with 3H leucine incorporation as described in Fitch et al. (41 ); see SI Appendix, SI Methods for details. Decays per minute were measured with a LS6500 liquid scintillation counter (Beckman Coulter, Brea, CA) with OptiPhase HiSafe 2 scintillation fluid and converted to bacterial production with standard eqs. (41 ). We also assessed the activity of four hydrolytic enzymes (β-1,4-glucosidase, β-D -1,4-cellobiosidase, β-1,4-xylosidase, and leucine aminopeptidase) and two oxidative enzymes (peroxidase and phenol oxidase) in porewater directly without dilution and without buffer to conserve in situ pH as described in Fitch et al. (41 ). Fluorescence and absorbance were read on a Synergy H1 microplate reader (BioTek, Winooski, VT).
Optiphase hisafe 2
Manufactured by Beckman Coulter
Sourced in United States
The OptiPhase HiSafe 2 is a liquid scintillation cocktail produced by Beckman Coulter. It is designed for use in liquid scintillation counting, a technique used to detect and quantify radioactive samples.
Automatically generated - may contain errors
Lab products found in correlation
Synergy h1, by Agilent Technologies (1 mentions)
Ls6500, by Beckman Coulter (1 mentions)
Acrylamide, by Bio-Rad (1 mentions)
Penicillin streptomycin, by Euroclone (1 mentions)
Temed, by Bio-Rad (1 mentions)
Horse radish peroxidase linked anti rabbit secondary antibodies, by Cell Signaling Technology (1 mentions)
Atcc ccl 185tm, by American Type Culture Collection (1 mentions)
Adenosine diphosphate (adp), by Merck Group (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
2 protocols using optiphase hisafe 2
1
Measuring Bacterial Production and Enzyme Activity
2
Enzymatic Activity Assay for NT5C2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
[8-14C]-IMP and [8-14C]-inosine were purchased from Moravek Biochemicals and Radiochemicals (Brea, CA, USA); DTT, ATP, ADP, AMP, MgCl2 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), 3,3′,5,5′-tetramethylbenzidine (TMB), STOP solution, primary antibody against NT5C2 and 2-deoxyglucose (2-dG) were from Sigma (Milano, Italy) and polyethyleneimine (PEI)-cellulose precoated thin-layer plastic sheets were obtained from Merck (Darmstadt, Germany) and prewashed once with 10% NaCl and three times with deionized water before use. DE-81 chromatographic paper was from Whatman (Madstone, UK), horse radish peroxidase (HRP)-linked anti-rabbit secondary antibodies were from Cell Signaling (Danvers, MA, USA) and scintillation liquid Optiphase Hisafe 2 was from Beckman (Brea, CA, USA). Acrylamide and TEMED were from Bio-Rad (Segrate, Milan); penicillin/streptomycin; glutamine and fetal bovine serum (FBS), RPMI and DMEM media were from Euroclone (Pero, Milan) and a Duolink kit for the proximity ligation assay (PLA) assay was from Olink Bioscience (St. Louis, MO, USA). The human lung carcinoma (A549) and the human astrocytoma (ADF) cell lines were purchased from ATCC (ATCC® CCL-185TM) and routinely tested for Mycoplasma contamination by PCR. All other reagents were of reagent grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!