Xevo tq ms detector

Manufactured by Waters Corporation

Sourced in United States

The Xevo TQ MS detector is a triple quadrupole mass spectrometer designed for quantitative and qualitative analysis. It offers high sensitivity, selectivity, and speed for a wide range of applications, including environmental, food, and pharmaceutical analysis.

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Lab products found in correlation

Xevo tq s mass spectrometer, by Waters Corporation (1 mentions) Prism 6, by GraphPad (1 mentions) Uhplc system, by Waters Corporation (1 mentions) Acquity uplc, by Waters Corporation (1 mentions)

Spelling variants of this product

Xevo TQ MS (53 citations) TQ Detector (30 citations) Xevo TQ Detector (2 citations) XevoTM (2 citations)

6 protocols using xevo tq ms detector

UPLC-MS/MS Quantification of Analytes

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The analytes were identified and quantified on a xevo TQ MS detector (Waters, Milford, MA, USA) coupled to the UPLC ACQUILITY system (Waters, Milford, MA, USA). In the chromatographic system, a CORTEX UPLC C18+ (2.1 mm × 100 mm, 1.6 μm) column was used for separation. The mobile phases were 0.2% formic acid in water (mobile phase A) and 0.2% formic acid in methanol (mobile phase B). A gradient program was started with 95% A and 5% B, with phase B increasing linearly to 30% in the first 4 min, linearly to 40% at 6 min and remaining constant for 2 min. The mobile phase then returned to the initial composition and equilibrated for 2 min prior to the next injection. The total run time for each sample was 10 min. The flow rate was 0.2 mL/min, and the injection volume was 5 μL. The column temperature was 60 °C.
MS/MS detection was carried out on a Waters xevo TQ-S mass spectrometer. MRM was used to measure the target analytes with the positive electrospray ionization (ESI+) mode. The MS conditions were as follows: capillary voltage, 3.0 kV; desolvation gas (600 L/h, 500 °C), nitrogen; collision gas (0.15 mL/min), argon. The MRM parameters for each compound and ion transition were optimized, as summarized in Table 1.

Zhang S., Zhao Y., Li H., Zhou S., Chen D., Zhang Y., Yao Q, & Sun C. (2016). A Simple and High-Throughput Analysis of Amatoxins and Phallotoxins in Human Plasma, Serum and Urine Using UPLC-MS/MS Combined with PRiME HLB μElution Platform. Toxins, 8(5), 128.

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Plasma 7-Ketocholesterol Measurement Protocol

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The plasma 7-KC level was evaluated as previously reported
[21 (link)]. Blood was collected in EDTA or heparin tube with the caution to avoid hemolysis, transported within 4 hours at room temperature to our lab, and kept at 4°C. Plasma were then separated at the same day of sample collecting and frozen at -80°C before analysis. Internal standard solution of 100 μL (CND Isotopes, Quebec, Canada) was added to 25 μl plasma. The mixture was vigorously mixed and centrifuged at a speed of 13,300 rcf for 5 min at room temperature and then the supernatant was transferred to a 96-well plate. Analysis was performed on a UPLC system with a Xevo TQ MS detector (Waters, USA). 7-KC and d7-7-KC were detected using positive electrospray ionization through multiple reaction monitoring modes. The normal range of 7-KC is 0 ~ 12.3 ng/mL (95^th percentile equals to 12.3, Maximum is 22.8)
[21 (link)].

Zhang H., Wang Y., Lin N., Yang R., Qiu W., Han L., Ye J, & Gu X. (2014). Diagnosis of Niemann-Pick disease type C with 7-ketocholesterol screening followed by NPC1/NPC2 gene mutation confirmation in Chinese patients. Orphanet Journal of Rare Diseases, 9, 82.

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Intracellular Drug Release in 4T1 Cells

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To explore the intracellular drug release, 4T1 cells were incubated in 24-well plates for 24 h (5 × 10⁴ cells/well). Then treated with DTX-solution or HPNAs (50, 100, and 200 ng mL⁻¹, equivalent to DTX) for 48 h and 72 h. Three samples in parallel for each concentration. Then, the cells and the culture medium were collected. The cells were disrupted by ultrasound. The concentrations of DTX were measured by UPLC-MS-MS system (Xevo TQ, Waters, USA) using software MassLynx V4.1. Xevo TQ (Waters, USA) is composed of a liquid phase system (ACQUITY Binary Solvent Manager Instrument) and a mass spectrometry system (Waters Xevo TQ MS Detector). The type of mass analyzer is the Triple Quadrupole. The liquid mobile phase was acetonitrile and water (v/v = 80: 20), both containing 0.1% formic acid, ran at a flow rate of 0.2 mL min⁻¹, and the sample volume was 5 μL. We used a reversed-phase chromatography column (Kinetex 2.6 μm XB-C18 50*2.1 mm) at the column temperature of 30 °C. The ESI source was used for mass spectrometry and electrospray+ for ion mode. The MSMS was adopted as the acquisition mode. The capillary was 3.45 kV, the cone was 64 V, the collision was 64, and the desolvation gas flow was 650 L h⁻¹.

Liu T., Li L., Wang S., Dong F., Zuo S., Song J., Wang X., Lu Q., Wang H., Zhang H., Cheng M., Liu X., He Z., Sun B, & Sun J. (2022). Hybrid chalcogen bonds in prodrug nanoassemblies provides dual redox-responsivity in the tumor microenvironment. Nature Communications, 13, 7228.

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Quantitative Bioanalysis of Compounds

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An Acquity U-HPLC system equipped with binary pumps, autosampler, inbuilt degasser and column heater coupled with Xevo TQ MS detector (Waters Corp, Milford, MA, USA) was used. A 10 µL sample loop in partial-loop with needle overfill injection mode was used to inject samples. The chromatographic system was controlled with MassLynx Software (V 4.1), and data was processed using the TargetLynx (V 4.1). Samples were centrifuged using an Eppendorf 5810R system (Eppendorf North America, Hauppauge, NY) and extracted samples were dried in Savant SPD1010 SpeedVac system (Thermo Scientific, Holbrook, NY). Calibration curves and graphs were plotted using Prism 6.01 (GraphPad Software Inc., La Jolla, CA).

Jamwal R., Topletz A.R., Ramratnam B, & Akhlaghi F. (2017). Ultra-high Performance Liquid Chromatography Tandem Mass-Spectrometry for Simple and Simultaneous Quantification of Cannabinoids. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1048, 10-18.

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Quantification of ECZ Skin Deposition

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Ultra-high-pressure liquid chromatography (UHPLC) coupled with tandem mass spectrometry detection was used to quantify ECZ deposited in skin during the in vitro delivery experiments. The UHPLC-MS/MS system consisted of a Waters Acquity UPLC^® system (Baden-Dättwil, Switzerland) with a binary solvent pump and sample manager and a Waters XEVO^® TQ-MS detector (Baden-Dättwil, Switzerland). Isocratic separation was performed using a Waters XBridge^® BEH column (C8, 2.1 × 50 mm, 2.5 µm). The column was thermostatted at 30 °C. The mobile phase consisted of (a) acetonitrile + 0.1% formic acid and (b) Milli-Q water + 0.1% formic acid (70:30 v/v). The flow rate was set at 0.2 mL/min and the injection volume was 5 µL. To account for the matrix effect, each injected sample contained MCZ (internal standard) at a concentration of 50 ng/mL. Tandem mass spectrometry was performed using electrospray ionization in positive mode and multiple reaction monitoring (MRM). MassLynx software was used for data integration and analysis. The UHPLC-MS/MS method was validated according to ICH guidelines (complete details are provided in the Supplementary Materials). The MS/MS settings for both ECZ and MCZ are presented in Table 1.

Quartier J., Capony N., Lapteva M, & Kalia Y.N. (2019). Cutaneous Biodistribution: A High-Resolution Methodology to Assess Bioequivalence in Topical Skin Delivery. Pharmaceutics, 11(9), 484.

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Measurement of Plasma 7-Ketocholesterol Levels

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The plasma 7-KC level was measured as previously described. 21, (link)22 (link) Plasma samples were routinely collected in tubes containing ethylene diamine tetraacetic acid K3 and butylated hydroxytoluene and immediately centrifuged. The plasma was isolated and stored at -80°C before analysis. The internal standard solution of 100 μL (CND Isotopes, Quebec, Canada) was added to 25 μL plasma. The mixture was vigorously mixed and centrifuged at a speed of 13 500 rcf for 5 min at room temperature, following which the supernatant was transferred to a 96-well plate. Analysis was performed on a ultraperformance liquid chromatography system with a Xevo TQ MS detector (Waters, Milford, MA). 7-KC was detected using positive electrospray ionization through multiple reaction monitoring modes. The recovery of plasma 7-KC was in the approximately range of 85% to 95%. All plasma samples were run in duplicate, and the values were averaged. The mean intra-and interassay coefficient of variation was 4.1% and 7.8%, respectively. The detection limit of this assay was 14.1 nmol/L, and no value was below the limit in our measurements. The plasma 7-KC level was measured within 1 month because the participant was recruited in the cohort. All stored samples were used under argon, which may have prevented occasional cholesterol oxidation products formation.

Song J., Wang D., Chen H., Huang X., Zhong Y., Jiang N., Chen C, & Xia M. (2017). Association of Plasma 7-Ketocholesterol With Cardiovascular Outcomes and Total Mortality in Patients With Coronary Artery Disease. Circulation research, 120(10).

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