Human serpin e1 pai 1 immunoassay

Isolated plasma was used in the assessment of a panel of biochemical parameters. The levels of PAI-1 were evaluated via a human SERPINE1/PAI-1 immunoassay (R&D Systems, Minneapolis, MN, USA). The levels of homocysteine were measured using the IMx fluorescence polarizing immunoassay (Abbott Laboratories, Abbott Park, IL, USA), and the levels of folate were measured using a radio-assay kit (ACS:180; Bayer, Tarrytown, NY, USA). Total cholesterol (T. chol) and uric acid levels were determined using commercially available enzymatic colorimetric tests (Roche Diagnostics GmbH, Mannheim, Germany). Platelet (PLT) counts were measured on a Sysmex XE2100 automated hematology analyzer (Sysmex Corporation, Kobe, Japan). Prothrombin time (PT) and activated partial thromboplastin time (aPTT) were measured using an automated photo-optical coagulometer ACL TOP (Mitsubishi Chemical Medience, Tokyo, Japan).

Blood samples were collected in anticoagulant containing collection tubes, and the sample tubes were centrifuged for 15 min at 1000× g to separate the plasma from whole blood. The PAI-1 levels were examined by the human Serpin E1/PAI-1 immunoassay (R&D Systems, Minneapolis, MN, USA). Homocysteine levels were measured using the IMx fluorescence polarizing immunoassay (Abbott Laboratories, Abbott Park, IL, USA), and folate levels were measured using a radio-assay kit (ACS:180; Bayer, Tarrytown, NY, USA). Total cholesterol and urate levels were determined using commercially available enzymatic colorimetric tests (Roche Diagnostics GmbH, Mannheim, Germany). Platelet counts were measured on a Sysmex XE2100 automated hematology analyzer (Sysmex Corporation, Kobe, Japan). Prothrombin time (PT) and activated partial thromboplastin (aPPT) were measured using an automated photo-optical coagulometer ACL TOP (Mitsubishi Chemical Medience, Tokyo, Japan).

Plasma PAI-1, total cholesterol, uric acid, and homocysteine levels were measured in participant blood samples. Plasma was separated by centrifugation of whole blood at 1000× g for 15 min. PAI-1 levels were determined using a human serpin E1/PAI-1 immunoassay (R&D Systems, Minneapolis, MN, USA). Uric acid and total cholesterol levels were measured using enzymatic colorimetric tests (Roche Diagnostics, GmbH, Mannheim, Germany). Homocysteine levels were measured using a fluorescence polarization immunoassay with an Abbott IMx analyzer (Abbott Laboratories, Abbott Park, IL, USA).