Antibodies against VDAC1 (B-6), IKKα/β (H-470), IKKβ (H-4), ΙΚΚγ (FL-419, B-3), HSP60 (K-19 and N-20), IκBα (C-21), Bcl-2, Bcl-xL, Cyclophilin D, c-Src, Enhanced green fluorescence protein (EGFP), MK2, p-MK2, and goat IgG were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies against p-IKKα/β, p-IκBα, ANT2 (E2B9D), Bax, p-Src (Y416), p-JNK (T183/Y185), JNK1/2, p38, and p-p38 were from Cell Signaling Technology (Danvers, MA, USA). Anti-cytochrome c antibody was from BD Bioscience (San Jose, CA, USA). Antibodies to Prx III and β-actin were obtained from AbFrontier (Seoul, Korea). Anti-MFF1 antibody was purchased from Proteintech (Rosemont, IL, USA). Recombinant GST-fused p38 enzyme was purchased from R&D systems (Minneapolis, MN, USA).
Mouse monoclonal antibody against α-tubulin, hydrogen peroxide, glucose oxidase, diamide, cyclosporine A, cycloheximide, coomassie brilliant blue R250, and DuoLink in situ fluorescence reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). SB203580, MG132, bongkrekic acid, lactacystin, bafilomycin A1, and 4,4’-diisothiocyanostilbene-2,2’-disulfonic acid) were purchased from Calbiochem (San Diego, CA, USA). Tetramethylrhodamine, ethyl ester (TMRE) was purchased from Invitrogen (Waltham, MA, USA). MK inhibitor IV was purchased from Cayman Chemical (Ann Arbor, MI, USA).
Mouse monoclonal antibody against α-tubulin, hydrogen peroxide, glucose oxidase, diamide, cyclosporine A, cycloheximide, coomassie brilliant blue R250, and DuoLink in situ fluorescence reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). SB203580, MG132, bongkrekic acid, lactacystin, bafilomycin A1, and 4,4’-diisothiocyanostilbene-2,2’-disulfonic acid) were purchased from Calbiochem (San Diego, CA, USA). Tetramethylrhodamine, ethyl ester (TMRE) was purchased from Invitrogen (Waltham, MA, USA). MK inhibitor IV was purchased from Cayman Chemical (Ann Arbor, MI, USA).