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8 protocols using ethylene glycol dimethacrylate egdma

1

Synthesis of Diclofenac-Carbopol Hydrogel

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Diclofenac sodium and ethylene glycol dimethacrylate (EGDMA) were purchased from Alfa-Aesar (Ward Hill, MA, USA). Carbopol 934 was obtained from Noveon, Inc (Cleveland, OH, USA). Acrylic acid was procured from Acros (Carlsbad, CA USA). Similarly, ammonium peroxydisulfate was obtained from Showa (Tokyo, Japan).
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2

Dual-responsive Superparamagnetic Hydrogel Nanocomposites

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Synthesis of dual-responsive
superparamagnetic hydrogel nanocomposites, AA-VSA-P/SPIONs, with different
concentration of SPIONs (Fe3O4) was conducted
using acrylic acid (AA) (Sigma-Aldrich), vinylsulfonic acid (VSA)
(Sigma-Aldrich) monomers, benzoyl peroxide (BPO) (Himedia) initiator,
and ethylene glycol dimethacrylate (EGDMA) (Alfa Aesar) cross-linker
along with synthesized SPIONs. To synthesize SPIONs, iron(II) chloride
tetrahydrate (FeCl2·4H2O) (Sigma-Aldrich)
and iron(III) chloride hexahydrate (FeCl3·6H2O) (Sigma-Aldrich) were used. The study on dye adsorption properties
of the superparamagnetic hydrogels, AA-VSA-P/SPIONs, was carried out
using methylene blue (MB) dye (Sigma-Aldrich). The required aqueous
solutions were prepared with deionized water, whereas the pH of aqueous
solutions was maintained by adding suitable amounts of HCl (0.05 M)
or NaOH (0.05 M) solutions (Merck).
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3

Synthesis of Ibuprofen-based Hydrogels

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Ibuprofen (IBP), alginic acid (AL) (Purity = 19 to 25% (carboxyl groups), MW = 10,000–600,000), aspartic acid (ASP) (Purity = 99 plus%, MW = 133.10) and acrylic acid (AA) (Purity = 98% extra pure, MW = 72.06 g/mol) were obtained from Across Organic (Carlsbad, CA, USA). Ammonium peroxodisulfate (APS) (Purity = 98%, MW = 228.21) was purchased from Showa (Tokyo, Japan). Similarly, ethylene glycol dimethacrylate (EGDMA) (Purity = 98%, MW = 198.22 g/mol) was purchased from Alfa-Aesar (Tewksbury, MA, USA).
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4

Synthesis of Antimicrobial Polymeric Materials

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Chrysin, 2,2′-azobisisobutyronitrile (AIBN) and acrylamide (AM) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Methyl alcohol and acetic acid were purchased from Chengdu Cologne Chemicals Co., Ltd. (Chengdu, China). Methacrylic acid (MAA) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Ethylene glycol dimethacrylate (EGDMA) was purchased from Alfa Aesar (Qingdao, China). Chloramphenicol and oxytetracycline were purchased from Shanghai Maclin Biochemical Technology Co., Ltd. (Chengdu, China).
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5

Magnetic Hydrogel Synthesis with Acrylic Acid and Vinylsulfonic Acid

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The poly(acrylic acid-co-vinylsulfonic acid) magnetic hydrogel was prepared using
acrylic acid (AA) (Sigma-Aldrich) and vinylsulfonic acid (VSA) (Sigma-Aldrich)
as monomers, along with benzoyl peroxide (BPO) (HiMedia) and ethylene
glycol dimethacrylate (EGDMA) (Alfa Aesar) as an initiator and a cross-linker,
respectively. For Fe3O4 nanoparticle synthesis,
iron(II) chloride tetrahydrate (FeCl2·4H2O) (Sigma-Aldrich) and iron(III) chloride (FeCl3·6H2O) (Sigma-Aldrich) were used. The required aqueous solutions
were prepared with deionized water, and the pH of aqueous solutions
was maintained by adding appropriate amounts of HCl (0.05 M) or NaOH
(0.05 M) solutions (Merck).
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6

Acetaminophen-based Polymer Synthesis

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Acetaminophen was obtained from Sigma-Aldrich (St. Louis, MO, USA). Acrylic acid (ACA; purity = 98%, extra pure, MW = 72.06 g/mol) and aspartic acid (APA; purity = 99 plus%, MW = 133.10) were purchased from Acros (Carlsbad, CA, USA). Similarly, ethylene glycol dimethacrylate (EGDMA; purity = 98%, MW = 198.22 g/mol) was procured from Alfa Aesar (Tewksbury, MA, USA), whereas ammonium persulfate (APS; purity = 98%, MW = 228.21) was obtained from Showa, Tokyo, Japan.
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7

PDMS-diol Hydrogel Preparation

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Poly(dimethylsiloxane) dialkanol (PDMS-diol, KF-6001) was purchased from Shin-Etsu Chemical Co. Ltd., Tokyo, Japan. 1-Vinyl-2-pyrrolidinone (NVP), tetraethyl orthosilicate (TEOS), and 2-hydroxy-2-methylbenzene acetone (D-1173) were purchased from Sigma-Aldrich (St. Louis, Mo USA). Further, 2-hydroxyethylmethacrylate (HEMA) and ethylene glycol dimethacrylate (EGDMA) were obtained from Acros Organics (NJ, USA). Phosphate buffered saline solution (PBS, 0.1 M, pH 7.4) was prepared in our laboratory.
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8

Synthesis of Polymeric Microspheres

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Methyl methacrylate (MMA), sodium nitrite, and poly(vinyl alcohol) (PVA, Mn = 124,000–186,000) were purchased from Sigma Aldrich (Seoul, South Korea). The MMA monomer was purified to remove the polymerization inhibitors. Ethylene glycol dimethacrylate (EGDMA) was purchased from Acros (Pittsburgh, PA, USA). 2,2-Azobisisobutyronitrile (AIBN) was purchased from Junsei Chemicals (Tokyo, Japan) and used after purification. Methylene chloride (MC) and benzene used as dispersion solvents were purchased from Daejung Chemicals & Metals (Siheung, South Korea). Ceramide (Y-30) and sodium dilauramidoglutamide lysine (DLGL, Pellicer L-30) were obtained from Cosmax (Seongnam, Korea).
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