Deuterium oxide 2h2o

Manufactured by Cambridge Isotopes

Sourced in Germany

Deuterium oxide (2H2O), also known as heavy water, is a stable isotope of water in which the hydrogen atoms are replaced by deuterium atoms. It is a colorless, odorless, and slightly heavier liquid than regular water. Deuterium oxide is used as a tracer in various scientific applications, including biochemistry, molecular biology, and pharmacology, to study metabolic processes and reaction kinetics.

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Lab products found in correlation

Sodium azide nan3, by Avantor (2 mentions) 3 trimethylsilyl propionic acid 2 2 3 3 d4 sodium salt tsp, by Thermo Fisher Scientific (2 mentions) Sodium phosphate, by Avantor (2 mentions) Hydrochloric acid (hcl), by Avantor (2 mentions) Thimerosal, by Merck Group (1 mentions) Sucrose, by Merck Group (1 mentions) Dithiothreitol (dtt), by GoldBio (1 mentions) Digoxin, by Thermo Fisher Scientific (1 mentions) Imidazole, by Thermo Fisher Scientific (1 mentions) N dodecyl β d maltoside, by Merck Group (1 mentions) Verapamil hydrochloride, by Fagron (1 mentions) Gsk591, by Merck Group (1 mentions) L arginine, by AppliChem (1 mentions) Sodium hydroxide (naoh), by Avantor (1 mentions) Chloroform, by Avantor (1 mentions) Adenosine, by Merck Group (1 mentions)

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Deuterium oxide (128 citations)

3 protocols using deuterium oxide 2h2o

NMR Metabolomics Sample Preparation

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Lactulose (Laevolac) was obtained from Fresenius Kabi Austria, D-Mannitol (Fluka), sucrose and thimerosal were obtained from Sigma Aldrich Austria. Sodium phosphate, dibasic (Na₂HPO₄), sodium hydroxide, hydrochloric acid (32% m/v), and sodium azide (NaN₃) were obtained from VWR International, 3-(trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) from Alfa Aesar (Karlsruhe, Germany), deuterium oxide (²H₂O) from Cambridge Isotope laboratories, Inc. (Tewksbury, MA).

Stryeck S., Horvath A., Leber B., Stadlbauer V, & Madl T. (2018). NMR spectroscopy enables simultaneous quantification of carbohydrates for diagnosis of intestinal and gastric permeability. Scientific Reports, 8, 14650.

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Purification of Membrane Proteins

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Verapamil hydrochloride was purchased from Fagron. Digoxin, ethylene glycol tetraacetic acid (EGTA) and imidazole were purchased from Alfa Aesar. The detergent used in protein purification, n-dodecyl-β-D-maltoside (DDM), was purchased from EMD Millipore Corporation. Escherichia coli total lipid extract powder was purchased from Avanti Polar Lipids Inc. DTT was purchased from Gold Biotechnology. Deuterium oxide (²H₂O) was purchased from Cambridge Isotope Laboratories. The remaining chemicals were purchased from Sigma–Aldrich.

Ledwitch K.V., Barnes R.W, & Roberts A.G. (2016). Unravelling the complex drug–drug interactions of the cardiovascular drugs, verapamil and digoxin, with P-glycoprotein. Bioscience Reports, 36(2), e00309.

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Quantitative Metabolite Profiling

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L-arginine was obtained from AppliChem (Germany), ω-N G , N G -asymmetric dimethylarginine (ADMA), ω-N G -N' G -symmetric dimethylarginine (SDMA), and ω-N G -monomethylarginine (MMA) were obtained from Santa Cruz Biotechnology, Inc. (Germany). Sodium phosphate, dibasic (Na2HPO4), sodium hydroxide, chloroform, hydrochloric acid (32% m/v), and sodium azide (NaN3) were obtained from VWR International, 3-(trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) from Alfa Aesar (Karlsruhe, Germany), deuterium oxide ( 2 H2O) from Cambridge Isotope laboratories, Inc. (Tewksbury, MA).
Adenosine, periodate oxidized (AdOx) and MS023 hydrochloride were obtained from Sigma Aldrich Austria. GSK591 (Synonyms: EPZ015866; GSK3203591 and GSK3368715 dihydrochloride (Synonyms: EPZ019997 dihydrochloride) were obtained from MedChemExpress Austria.

Zhang F., Kerbl-Knapp J., Colman M.J.R., Macher T., Vujić N., Fasching S., Jany-Luig E., Korbelius M., Kuentzel K.B., Mack M., Akhmetshina A., Paar M., Rinner B., Hörl G., Steyrer E., Stelzl U., Burgering B., Eisenberg T., Pertschy B., Kratky D., & Madl T. (2021). Global analysis of protein arginine methylation.

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