α cd8 percp

Manufactured by BioLegend

α-CD8-PerCP is a fluorochrome-conjugated antibody that binds to the CD8 cell surface marker. It is designed for use in flow cytometry applications to identify and quantify CD8-positive cells within a sample.

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Lab products found in correlation

Clone 145 2c11, by Thermo Fisher Scientific (1 mentions) Clone 53 6, by BioLegend (1 mentions) Robosep buffer, by STEMCELL (1 mentions) Clone m1 70, by BioLegend (1 mentions) α cd3 fitc, by Thermo Fisher Scientific (1 mentions) αcd3 apc h7, by BD (1 mentions)

Spelling variants of this product

CD8-PerCP (30 citations) α-CD8 (5 citations)

2 protocols using α cd8 percp

Purification and Transfer of T-cell Subsets

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One week after the third recall, lymphocytes were collected from spleen of immunized or control group mice. Pelleted cells were resuspended in RobosepTM buffer (Stemcell technologies) at a final concentration of 10⁸ cells/mL. 5*10⁸ cells per group were purified by CD3+, CD8+ or CD4+ negative selection using an EasySep isolation kit (Stemcell Technologies). Cells were then injected intravenously (IV) in 4–5 week old C57BL/6 mice (10⁷ cell in 300μl). The remaining cells were marked with α-CD3-FITC (1:1000, clone 145-2C11, eBioscience), α-CD4-BrilliantViolet421 (1:500, clone M1/70, Biolegend) or α-CD8-PerCP (1:500, clone 53–6.7, Biolegend) stain and analyzed by FACS to assess the purity of the cell suspension, purity was always superior to 95%.

Castiglioni P., Hartley M.A., Rossi M., Prevel F., Desponds C., Utzschneider D.T., Eren R.O., Zangger H., Brunner L., Collin N., Zehn D., Kuhlmann F.M., Beverley S.M., Fasel N, & Ronet C. (2017). Exacerbated Leishmaniasis Caused by a Viral Endosymbiont can be Prevented by Immunization with Its Viral Capsid. PLoS Neglected Tropical Diseases, 11(1), e0005240.

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Antigen-specific CD8+ T cell isolation

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Antigen-specific CD8 + T cells were labeled with optimal concentrations of the indicated HLA-A*11:01p tetramers (PE or APC) for 20 min at 37 °C, and then stained with the following monoclonal antibodies (mAbs) for 30 min at 4 °C: (i) αCD8-BV711 or αCD8-PerCP (BioLegend); (ii) αCD3-APC-H7, αCD14-V500, and αCD19-V500 (BD Biosciences); and, in some experiments, (iii) αTRBV11-2-FITC (Beckman Coulter). Non-viable events were excluded using Fixable Aqua or Violet Dead Cell Stain Kits (Thermo Fisher Scientific).

Culshaw A., Ladell K., Gras S., McLaren J.E., Miners K.L., Farenc C., van den Heuvel H., Gostick E., Dejnirattisai W., Wangteeraprasert A., Duangchinda T., Chotiyarnwong P., Limpitikul W., Vasanawathana S., Malasit P., Dong T., Rossjohn J., Mongkolsapaya J., Price D.A, & Screaton G.R. (2017). Germline bias dictates cross-serotype reactivity in a common dengue-virus-specific CD8⁺ T cell response. Nature immunology, 18(11).

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