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Tissue culture plasticware

Manufactured by Thermo Fisher Scientific
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Sourced in Denmark

Tissue culture plasticware is a range of specialized laboratory equipment designed for the cultivation and maintenance of cells, tissues, and organisms in a controlled in vitro environment. These products provide a standardized, sterile, and optically clear surface for cell attachment, growth, and observation.

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Lab products found in correlation

Polymerized collagen 1, by BD (2 mentions) Gelatin, by Merck Group (2 mentions) Egm 2mv medium, by Lonza (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Adenine hemisulfate, by Merck Group (1 mentions) Insulin solution, by Merck Group (1 mentions) Dmem low glucose media, by Thermo Fisher Scientific (1 mentions) Dmem high glucose media, by Thermo Fisher Scientific (1 mentions) Ham s f12 media, by Thermo Fisher Scientific (1 mentions) Nuclei ez prep kit, by Merck Group (1 mentions) Charcoal stripped fbs css, by Thermo Fisher Scientific (1 mentions) Zapoglobin, by Beckman Coulter (1 mentions) Isoton 2, by Beckman Coulter (1 mentions) Horseradish peroxidase conjugated donkey anti rabbit and sheep anti mouse antibodies, by GE Healthcare (1 mentions) Apo transferrin, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) D biotin, by Merck Group (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Cell culture media, by Thermo Fisher Scientific (1 mentions) Glass coverslips 18 mm circular, by Thermo Fisher Scientific (1 mentions)

6 protocols using tissue culture plasticware

1

Cell Culture Media and Reagents Procurement

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DMEM low glucose media, DMEM high glucose media, Hams’ F‐12 media, DMEM/F‐12 media (1:1), penicillin/streptomycin solution and phosphate buffered saline (PBS) were purchased from Invitrogen (Carlsbad, CA). The media additives d‐biotin, adenine hemisulfate, insulin solution, apo‐transferrin, and Nuclei EZ Prep kit were purchased from Sigma–Aldrich (St. Louis, MO). Fetal bovine serum (FBS) was obtained from HyClone (Logan, UT). Charcoal stripped FBS (CSS) was prepared within our laboratory or purchased from Invitrogen (Carlsbad, CA). Zapoglobin and Isoton II were purchased from Beckman Coulter Inc. (Fullerton, CA). Rabbit anti‐mouse IgG secondary antibody was obtained from Zymed Laboratories, Inc. Both horseradish peroxidase‐conjugated donkey anti‐rabbit and sheep anti‐mouse antibodies were purchased from GE Healthcare Biosciences (Pittsburg, PA). All tissue culture plasticware and additional chemicals were purchased from Fisher Scientific (Suwanee, GA).
Olokpa E., Bolden A, & Stewart L.V. (2016). The Androgen Receptor Regulates PPARγ Expression and Activity in Human Prostate Cancer Cells. Journal of Cellular Physiology, 231(12), 2664-2672.
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2

Mechanotransduction Pathways in Osteogenic Differentiation

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All materials were purchased from Sigma unless otherwise noted. Tissue culture plastic ware and glass coverslips (18-mm circular) were purchased from Fisher Scientific. Cell culture media and reagents were purchased from Gibco. Rabbit anti-Runx2 (ab23981) and anti-Osteopontin (ab8448) were purchased from Abcam. Mouse anti-MyoD (MAB3878) Mouse anti-α5β1 (MAB1969) and αVβ3 (MAB1976Z) were purchased from Millipore. Blebbistatin, Y-27632, FR180204 (ERK inhibitor), SP600125 (JNK inhibitor), and SB202190 (p38 inhibitor) were purchased from Calbiochem. Tetramethylrhodamine-conjugated anti-rabbit IgG antibody, Alexa Fluor 647-conjugated anti-mouse IgG antibody, Alexa Fluor 555-conjugated anti-rabbit IgG antibody, Alexa488-phalloidin and 4,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen.
Lee J., Abdeen A.A., Tang X., Saif T.A, & Kilian K.A. (2015). Geometric guidance of integrin mediated traction stress during stem cell differentiation. Biomaterials, 69, 174-183.
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3

Culturing ER+ Breast Cancer Cells

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ER+, endocrine sensitive MCF-7 and T47D cells were routinely cultured in glutamine-supplemented RPMI medium (Invitrogen, Paisley, UK), supplemented with 5% foetal calf serum (FCS), antibiotics (10I U/ml penicillin and 10 μg/ml streptomycin) and fungi-zone (2.5 μg/ml), and incubated at 37°C with 5% carbon dioxide. For experimental analysis, the medium was changed to experimental medium, containing phenol-red-free RPMI supplemented with 5% FCS, 100 mM glutamine and antibiotics as above. For estrogen withdrawal conditions (-E2) the FCS within the experimental medium was replaced with 5% charcoal-stripped, steroid-depleted FCS, while for anti-estrogen and hormone treatments the medium was supplemented with 10-9 M estradiol (E2), 10-7 M 4-hydroxytamoxifen (‘Tam’) or 10-7 M fulvestrant (‘Fas’). All tissue culture media and constituents were obtained from Life Technology Europe Ltd (Paisley, UK) and tissue culture plasticware was obtained from Nunc (Rosklide, Denmark).
Rees M., Smith C., Barrett-Lee P, & Hiscox S. (2020). PELP-1 regulates adverse responses to endocrine therapy in Estrogen Receptor (ER) positive breast cancer. Oncotarget, 11(51), 4722-4734.
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4

Optimized Cell Culture Reagents

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Tissue culture plasticware was from Nunc (Roskilde, Denmark). Bovine serum albumin (BSA), epidermal growth factor (EGF), hydrocortisone and bovine insulin were from Sigma-Aldrich (St Louis, MO, USA). Cholera enterotoxin was from List Biologicals (Campbell, CA, USA). Fetal bovine serum (FBS) was from AusGeneX (Oxenford, QLD, Australia). RPMI 1640 medium was purchased from Thermo Scientific (Rockford, IL). DMEM/F12 medium, horse serum and trypsin/EDTA were from Gibco/Life Technologies (Mulgrave, VIC, Australia). Antibodies against PARP, total p53 and phospho-p53 (Ser-15) were purchased from Cell Signaling Technology (Beverly, MA, USA), while the antibody against p21 was from Merck Millipore (Billerica, MA, USA). Antibodies R30 and R100 against full-length human IGFBP-3 were generated in-house [68 (link)]. The β-actin antibody was from Sigma-Aldrich. Molecular weight markers PageRuler (Fermentas Life Sciences, Burlington, ON, Canada) or Himark (Invitrogen, Carlsbad, CA) were used for Western blot analysis.
Marzec K.A., Lin M.Z., Martin J.L, & Baxter R.C. (2015). Involvement of p53 in insulin-like growth factor binding protein-3 regulation in the breast cancer cell response to DNA damage. Oncotarget, 6(29), 26583-26598.
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5

Culturing Brain Microvascular Endothelial Cells

Cited 1 time
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ECs were cultured on tissue culture plasticware (Nunc, Roskilde, Denmark) coated with polymerized collagen I (BD Biosciences, Oxford, U.K.). The immortalized rat brain MVEC line GPNT was maintained as previously described (10 (link)). Primary cultures of rat brain MVECs (23 (link)) and the human brain MVEC line hCMEC/D3 (24 (link)) were cultured in EGM2-MV medium (Lonza, Slough, U.K.). Human dermal MVEC (PromoCell) were cultured on tissue culture plasticware coated with gelatin (Sigma) and maintained in EC growth media MV2 (PromoCell). Quality of EC cultures was routinely assessed by visual inspection and transendothelial electrical resistance (TEER) measurements (23 (link)). Importantly, none of the treatments (pharmacological antagonists or transfections) had any effect on EC monolayer integrity.
Dragoni S., Hudson N., Kenny B.A., Burgoyne T., McKenzie J.A., Gill Y., Blaber R., Futter C.E., Adamson P., Greenwood J, & Turowski P. (2017). Endothelial MAPKs Direct ICAM-1 Signaling to Divergent Inflammatory Functions. The Journal of Immunology Author Choice, 198(10), 4074-4085.
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6

Culturing Endothelial Cells for In Vitro Studies

Cited 1 time
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ECs were cultured on tissue culture plastic ware (Nunc; Roskilde, Denmark) coated with polymerized collagen I (BD Biosciences, Oxford, UK). The immortalized rat BMVEC line GPNT was maintained as previously described (10 (link)). Primary cultures of rat BMVECs (23 (link)) and the human BMVEC line hCMEC/D3 (24 (link)) were cultured in EGM®2-MV medium (Lonza; Slough, UK). Human DMEC (PromoCell) were cultured on tissue culture plastic ware coated with gelatin (Sigma, UK) and maintained in endothelial cell growth media MV2 (PromoCell). Quality of EC cultures was routinely assessed by visual inspection and TEER measurements (23 (link)). Importantly, none of the treatments (pharmacological antagonists or transfections) had any effect on EC monolayer integrity.
Dragoni S., Hudson N., Kenny B.A., Burgoyne T., McKenzie J.A., Gill Y., Blaber R., Futter C.E., Adamson P., Greenwood J, & Turowski P. (2017). Endothelial MAPKs Direct ICAM-1 Signaling to Divergent Inflammatory Functions. The Journal of Immunology Author Choice, 198(10), 4074-4085.
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