Laser microdissection 7000 system

Cryotome (Thermo Shandon As620 Cryotome, Cheshire, UK), Cryogen (Thermo Shandon, Cheshire, UK), Non-fluorescent polyethylene terephthalate (PET) microscope steel frame slide (76 × 26 mm, 1.4 μm, Leica Microsystems, Bensheim, Germany), Leica Laser microdissection 7000 system, 500 μL micro-centrifuge tube (Leica), Centrifuge (Centrifuge 5417R, Eppendorf, Hamburg, Germany), Ultrasonic instrument (CREST 1875HTAG Ultrasonic Processor, CREST, Trenton, NJ), HPLC grade vial (1.5 mL, Grace, Hong Kong), Glass-lined pipe with plastic ring (400 μL, Grace, Hong Kong), Electronic balance (Mettler Toledo MT5 style), Agilent 6540 ultra-definition accurate mass quadrupole time-of-flight spectrometer equipped with a mass hunter workstation software (Agilent version B.06.00 series, Agilent Technologies, USA), Acquity UPLC BEH C₁₈ column (2.1 mm × 100 mm, 1.7 μm) coupled with a C₁₈ pre-column (2.1 mm × 5 mm, 1.7 μm, Waters, USA).

Leica Laser microdissection 7000 system (Leica, Benshein, Germany), Agilent 6540 ultra-performance liquid chromatography quadrupole time of flight spectrometer equipped with a mass hunter workstation software (Agilent version B.06.00 series, Agilent Technologies, USA), Cryotome (Thermo Shandon As620 Cryotome, Cheshire, UK), Ultrasonic instrument (CREST 1875HTAG Ultrasonic Processor, CREST, Trenton, NJ), Centrifuge (Centrifuge 5417R, Eppendorf, Hamburg, Germany), Electronic balance (Mettler Toledo MT5 style), Nonfluorescent polyethylene terephthalate (PET) microscope steel frame slide (76 × 26 mm, 1.4 μm, Leica Microsystems, Bensheim, Germany), Centrifuge tube (500 μL, 1.5 mL, Leica), HPLC grade vial (1.5 mL, Grace, Hong Kong), glass insert with plastic bottom spring (400 μL, Grace, Hong Kong), Acquity UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm, Waters, USA), C18 pre-column (2.1 × 5 mm, 1.7 μm, Waters, USA).