All animal experiments were performed under protocols approved by the Institutional Animal Care and Use Committee of the Feinstein Institute for Medical Research, North Shore-LIJ Health System, the Karolinska Institutet or the University Health Network Animal Care Committee.
Choline acetyltransferase (ChAT)-GFP (B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J), ChAT-floxed (B6.129-Chattm1Jrs/J), and mice expressing Cre recombinase under the control of the endogenous CD4 promoter (CD4-Cre) were purchased from Jackson Laboratories (Bar Harbor, ME, USA). ChAT-floxed and CD4-Cre mice were crossed to generate mice genetically devoid of ChAT in the CD4+ population. Animals were housed at 25°C on a 12-hour light/dark cycle, and acclimatized for at least one week before conducting experiments. Water and regular rodent chow were available ad libitum. BALB/c nude (nu/nu) mice 8 to 12 weeks-old were obtained from Taconic. eNOS deficient mice16 (link) were provided by Drs. Jon Lundberg and Eddie Weitzberg, Karolinska Institutet, Stockholm, Sweden. Cells pooled from ChAT(BAC)-eGFP male and female mice were used for functional and phenotypic characterization. Animals were euthanized using CO2 asphyxiation or cervical dislocation.
Choline acetyltransferase (ChAT)-GFP (B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J), ChAT-floxed (B6.129-Chattm1Jrs/J), and mice expressing Cre recombinase under the control of the endogenous CD4 promoter (CD4-Cre) were purchased from Jackson Laboratories (Bar Harbor, ME, USA). ChAT-floxed and CD4-Cre mice were crossed to generate mice genetically devoid of ChAT in the CD4+ population. Animals were housed at 25°C on a 12-hour light/dark cycle, and acclimatized for at least one week before conducting experiments. Water and regular rodent chow were available ad libitum. BALB/c nude (nu/nu) mice 8 to 12 weeks-old were obtained from Taconic. eNOS deficient mice16 (link) were provided by Drs. Jon Lundberg and Eddie Weitzberg, Karolinska Institutet, Stockholm, Sweden. Cells pooled from ChAT(BAC)-eGFP male and female mice were used for functional and phenotypic characterization. Animals were euthanized using CO2 asphyxiation or cervical dislocation.