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K2hpo4 3h2o

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K2HPO4 · 3H2O is a chemical compound that is a crystalline form of potassium phosphate. It is a white, crystalline solid that is soluble in water.

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Lab products found in correlation

Agarose, by Thermo Fisher Scientific (2 mentions) Fm4 64, by Thermo Fisher Scientific (2 mentions) Softworx v5, by Cytiva (2 mentions) Kh2po4, by Thermo Fisher Scientific (2 mentions) Nh4 2so4, by Thermo Fisher Scientific (2 mentions) C6h5o7na3 2h2o, by Thermo Fisher Scientific (2 mentions) Sytox green, by Thermo Fisher Scientific (2 mentions) Lb broth, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions) Mgcl2 6h2o, by Thermo Fisher Scientific (1 mentions) Nahco3, by Thermo Fisher Scientific (1 mentions) Potassium chloride (kcl), by Merck Group (1 mentions) Na2so4, by Merck Group (1 mentions) Cacl2, by Merck Group (1 mentions)

Close spelling variants of this product

K2HPO4

3 protocols using k2hpo4 3h2o

1

Synthetic Blood Fluid Composition

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Kokubo’s SBF was created based on a protocol developed for analysing apatite formation [42 (link)]. Reagents were mixed in in 3 L of distilled water in the following order: NaCl (Fisher Scientific) (24.105 g), NaHCO3 (Fisher Scientific) (1.065 g), KCl (Sigma) (0.675 g), K2HPO4·3H2O (0.693 g), MgCl2·6H2O (Fisher Scientific) (0.933 g) 1.0M HCl (117 mL), CaCl2 (Sigma) (0.876 g), Na2SO4 (Sigma) (0.216 g), Tris(hydroxymethyl)aminomethane (Tris) (Fisher Scientific) (18.354 g), 1.0M HCl (Fisher Scientific) (0–15 mL). Tris and HCl were added concurrently to maintain a pH of ~7.4.
Deen I, & Rosei F. (2019). Silk fibroin-derived polypeptides additives to promote hydroxyapatite nucleation in dense collagen hydrogels. PLoS ONE, 14(7), e0219429.
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2

GAS Cell Morphology Visualization

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To visualize the morphology of GAS cells, bacteria from late stationary (overnight cultures) or exponential (4 hours of growth post 1:20 back-dilution of overnight culture in fresh medium) phase of growth were mixed by vortexing. 6 μl of each cell suspension was added to 1.5 μl dye mix composed of 60 μg/mL FM4–64 (Life Technologies Corporation), 10 μg/mL DAPI (Sigma Aldrich), and 2.5 μM SYTOX Green (Life Technologies Corporation) in 1X T-base (2 g (NH4)2SO4 [Fisher Scientific], 18.3 g K2HPO4 ⋅ 3H2O [Fisher Scientific], 6 g KH2PO4 [Fisher Scientific], 1 g C6H5O7Na3 ⋅ 2H2O [Fisher Scientific] per 1 L of ddH2O) and transferred onto an agarose pad (20% LB broth, 1% agarose [Fisher Scientific]). Samples were air-dried under a fume hood (care was taken to prevent over-drying). Cells were visualized on an Applied Precision DV Elite optical sectioning microscope equipped with a Photometrics CoolSNAP-HQ2 camera. Pictures were deconvolved using SoftWoRx v5.5.1 (Applied Precision). Images for figures were prepared using FIJI. Bacterial cell diameters from multiple pictures were quantified with CellProfiler (Kamentsky et al., 2011 (link)) on two separate occasions.
Wierzbicki I.H., Campeau A., Dehaini D., Holay M., Wei X., Greene T., Ying M., Sands J.S., Lamsa A., Zuniga E., Pogliano K., Fang R.H., LaRock C.N., Zhang L, & Gonzalez D.J. (2019). Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion. Cell reports, 29(10), 2979-2989.e15.
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3

GAS Cell Morphology Visualization

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To visualize the morphology of GAS cells, bacteria from late stationary (overnight cultures) or exponential (4 hours of growth post 1:20 back-dilution of overnight culture in fresh medium) phase of growth were mixed by vortexing. 6 μl of each cell suspension was added to 1.5 μl dye mix composed of 60 μg/mL FM4–64 (Life Technologies Corporation), 10 μg/mL DAPI (Sigma Aldrich), and 2.5 μM SYTOX Green (Life Technologies Corporation) in 1X T-base (2 g (NH4)2SO4 [Fisher Scientific], 18.3 g K2HPO4 ⋅ 3H2O [Fisher Scientific], 6 g KH2PO4 [Fisher Scientific], 1 g C6H5O7Na3 ⋅ 2H2O [Fisher Scientific] per 1 L of ddH2O) and transferred onto an agarose pad (20% LB broth, 1% agarose [Fisher Scientific]). Samples were air-dried under a fume hood (care was taken to prevent over-drying). Cells were visualized on an Applied Precision DV Elite optical sectioning microscope equipped with a Photometrics CoolSNAP-HQ2 camera. Pictures were deconvolved using SoftWoRx v5.5.1 (Applied Precision). Images for figures were prepared using FIJI. Bacterial cell diameters from multiple pictures were quantified with CellProfiler (Kamentsky et al., 2011 (link)) on two separate occasions.
Wierzbicki I.H., Campeau A., Dehaini D., Holay M., Wei X., Greene T., Ying M., Sands J.S., Lamsa A., Zuniga E., Pogliano K., Fang R.H., LaRock C.N., Zhang L, & Gonzalez D.J. (2019). Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion. Cell reports, 29(10), 2979-2989.e15.
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