Pwr 1e

Manufactured by Thermo Fisher Scientific

The PWR-1E is a compact and versatile laboratory power supply designed for various applications. It provides a stable and reliable source of direct current (DC) power, with an output range of 0-30V and 0-5A. The PWR-1E features precise voltage and current control, as well as over-voltage and over-current protection for safe operation.

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Lab products found in correlation

6 protocols using pwr 1e

Prostate Cell Line Maintenance Protocols

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Prostate (RWPE-1, RWPE-2, PWR1E, VCaP, DU145, PC3, PC3M) and phoenix cell lines were obtained from the American Type Culture Collection (Manassas, VA). Myc-CaP and Prostatic Intraepithelial Neoplasia (PIN) cell lines were a generous gift from Dr. Charles Sawyers (Memorial Sloan Kettering, New York, NY) and Dr. Mark Stearns (Drexel University, Philadelphia, PA) respectively. All cells were maintained at 37°C with 5% CO₂. RWPE-1, RWPE-2, PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP, VCaP, DU145, PC3, and PC3M cells were cultured in Dulbecco’s modified Eagle’s medium (HyClone, GE Healthcare Life Sciences, Logan, UT) supplemented with 10% fetal bovine serum (HyClone, GE Healthcare Life Sciences, Logan, UT) and 0.5% penicillin-streptomycin (Life Technologies, Grand Island, NY). Cells were regularly tested for mycoplasma with MycoAlert per manufacturer’s protocol (LT07-318, Lonza, Basel, Switzerland).

Izadmehr S., Fernandez-Hernandez H., Wiredja D., Kirschenbaum A., Lee-Poturalski C., Tavassoli P., Yao S., Schlatzer D., Hoon D., Difeo A., Levine A.C., Mosquera J.M., Galsky M.D., Cordon-Cardo C, & Narla G. (2024). Cooperativity of c-MYC with Krüppel-Like Factor 6 Splice Variant 1 induces phenotypic plasticity and promotes prostate cancer progression and metastasis. bioRxiv.

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Prostate Cancer Cell Line Cultivation and Characterization

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Androgen-insensitive
(DU145 ACC 261, PC-3 ACC 465) and androgen-sensitive (LNCaP ACC 256)
human prostate cancer cell lines were purchased from the German Collection
of Microorganisms and Cell Cultures (DSMZ). Noncancerous human prostate
epithelial cell lines (PWR-1E CRL-11611, RWPE-1, CRL-11609) were purchased
from the American Type Culture Collection (ATCC). DU145, PC-3, and
LNCaP cells were cultured using RPMI 1640 medium with GlutaMAX (Gibco),
supplemented with 10% fetal bovine serum (FBS) (Gibco). PWR-1E and
RWPE-1 cells were cultured using keratinocyte serum-free medium supplemented
with l-glutamine, bovine pituitary extract (50 mg/L), and
epidermal growth factor (5 μg/L) (Gibco). Cells were maintained
in a humidified 5% CO₂ incubator at 37 °C.
CBD
was provided by GreenLight Pharmaceuticals. CBD purity of >99.7%
was
confirmed by convergence chromatography. SR141716 (CB₁ antagonist),
SR144528 (CB₂ antagonist), capsazepine (TRPV1 antagonist),
LPI (GPR55 agonist), and staurosporine (apoptosis positive control)
were purchased from Sigma-Aldrich. CBD, SR141716, SR144528, and capsazepine
were dissolved by using dimethyl sulfoxide (DMSO) (PanReac AppliChem).
LPI was dissolved using sterile dH₂O. All drug compounds
were stored according to the manufacturer’s instructions.

O’Reilly E., Khalifa K., Cosgrave J., Azam H., Prencipe M., Simpson J.C., Gallagher W.M, & Perry A.S. (2023). Cannabidiol Inhibits the Proliferation and Invasiveness of Prostate Cancer Cells. Journal of Natural Products, 86(9), 2151-2161.

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Cell Culture Conditions for Prostate Cell Lines

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All cell lines were obtained from ATCC, and were incubated at 37 °C in a humidified atmosphere with 5% CO₂. LNCaP (CRL1740) and 22Rv1 (CRL2505) cell lines were cultured in RPMI Medium (GIBCO Life Technologies, Grand Island, NY). PC3 (CRL1435) cell line was cultured in Dubelcco’s modified Eagle’s Medium (DMEM) (#12400-024, GIBCO). Both media were supplemented with 10% FBS, F-12 and penicillin-streptomycin. RWPE-1 (CRL11609), RWPE-2 (CRL11610) and PWR-1E (CRL11611) cell lines were maintained in KSFM Medium, supplemented with Bovine pituitary extract (BPE) (0,05 mg/mL) and epithelial growth factor (EGF) (5 ng/mL)(#17005-042, GIBCO).

Farfán N., Ocarez N., Castellón E.A., Mejía N., de Herreros A.G, & Contreras H.R. (2018). The transcriptional factor ZEB1 represses Syndecan 1 expression in prostate cancer. Scientific Reports, 8, 11467.

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Prostate Cell Lines: Culture and Treatment

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The prostate cancer cells LNCaP, 22RV1, and VCaP were acquired from the Tissue Culture and Biobanking Shared Resource, and the normal prostate cells PWR-1E were acquired from Dr. S. Byers's laboratory at Georgetown University. All cell lines were originally obtained from the American Type Culture Collection. The prostate cancer cell lines LNCaP, 22RV1, and VCaP were maintained in an RPMI medium 1640 (Gibco; Life Technologies) containing 10% of fetal bovine serum (FBS) and 1% of sodium pyruvate. The normal prostate cell line PWR-1E was maintained in keratinocyte-serum-free media supplemented with L-glutamine, human recombinant epidermal growth factor, and bovine pituitary extract (Gibco; Life Technologies). Prior to treatment, PWR-1E, LNCaP, and VCaP cells were plated in improved minimum essential media (IMEM)-containing phenol red and 10% FBS serum. At 70% confluence, the media were changed to IMEM phenol red-free, lipoic acid-free media (Crystalgen) containing 5% charcoal-treated calf serum (CCS; Valley Biomedical) for 48 hours. The cells were treated with dihydroxytestosterone (DHT; 5 nM), the synthetic androgen R1881 (R1881; 5 nM), calcium (calcium chloride; 1 mM and 3 mM), the antiandrogens hydroxyflutamide (HF; 10 μM), bicalutamide (BIC; 10 μM), and enzalutamide (ENZ; 10 μM).

Sharawi Z.W., Khatrawi S.M., Wang Q., Zhou H., Cyrus K., Yan G., Hoxter B., Haddad B.R, & Martin M.B. (2023). Calcium Activation of the Androgen Receptor in Prostate Cells. International Journal of Endocrinology, 2023, 9907948.

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Prostate Cell Line Cultivation and Authentication

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Non-malignant epithelial prostate cell lines (RWPE-1 and PWR-1E) and prostate carcinoma cell lines (LNCaP, Du145, PC3) were obtained from the American Type Culture Collection (ATCC) and cultured under recommended conditions as described previously (28 (link)). RWPE-1 and PWR-1E cells were cultured in keratinocyte growth medium supplemented with 5 ng/mL human recombinant epidermal growth factor, 0.05 mg/mL bovine pituitary extract (Invitrogen). LNCaP, Du145, PC3 were maintained in RPMI 1640 media supplemented with 10% fetal bovine serum (FBS) (Atlanta biologicals) and 1% penicillin/streptomycin. Cell lines were maintained in an incubator with a humidified atmosphere of 95% air and 5% CO2 at 37°C.
Cell lines were authenticated by DNA short-tandem repeat analysis by ATCC. The experiments with cell lines were performed within 6 months of their procurement/resuscitation.

Saini S., Majid S., Shahryari V., Tabatabai Z.L., Arora S., Yamamura S., Tanaka Y., Dahiya R, & Deng G. (2014). Regulation of SRC kinases by microRNA-3607 located in a frequently deleted locus in prostate cancer. Molecular cancer therapeutics, 13(7), 1952-1963.

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Prostate Cell Line Culture Protocol

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Human prostate carcinoma cell lines, PC3 and DU145, and the normal epithelial prostate cell line, PWR-1E, were obtained from the American Type Culture Collection (Rockville, MD). The prostate cancer cell lines were cultured in RPMI-1640 (Hyclone, Logan, UT) supplemented with 10% fetal bovine serum (Invitrogen, Carlsbad, CA), 50 mg/ml penicillin and 50 mg/ml streptomycin (Invitrogen, Carlsbad, CA), and maintained in an incubator with a humidified atmosphere of 95% air and 5% CO₂ at 37°C. The PWR-1E cells were cultured in keratinocyte growth medium supplemented with 5 ng/ml human recombinant epidermal growth factor and 0.05 mg/ml bovine pituitary extract (Invitrogen, Carlsbad, CA) and maintained in an incubator under the conditions described above.

Chaudhary P, & Vishwanatha J.K. (2014). c-Jun NH2-terminal kinase-induced proteasomal degradation of c-FLIPL/S and Bcl2 sensitize prostate cancer cells to Fas- and mitochondria-mediated apoptosis by tetrandrine. Biochemical pharmacology, 91(4), 457-473.

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