Following Thompson’s protocol for beluga whale blow sampling [43 (link)], polystyrene petri dishes (Item No.: 633181, Greiner Bio-One International GmbH, Kremsmünster, Austria) were covered with nylon mesh of 110 μm pore size (Elko Filtering Co., Miami, FL, USA) fixed with a tight elastic band. Both petri dishes and membranes were additionally wiped down with 70% alcohol to prevent external contamination during assembly. Hereafter, this device will be referred to as “Nitex” (Figure 1 A).
Polystyrene petri dishes
Manufactured by Greiner
Sourced in Austria
Polystyrene petri dishes are a type of laboratory equipment used for culturing cells, microorganisms, and other biological samples. They provide a sterile, controlled environment for growth and observation. The dishes are made of transparent polystyrene plastic and come in various sizes to accommodate different experimental needs.
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Lab products found in correlation
4 protocols using polystyrene petri dishes
1
Beluga Whale Blow Sampling Protocol
2
Immobilizing Quorum Quenching Proteins
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Polystyrene Petri dishes 35 × 10 mm (Greiner, Kremsmünster, Austria) were coated by the company Surflay Nanotec (Berlin,Germany) with ethyleneiminepolymers (PEI) according to the previously published Layer-by-Layer method52 (link). Immobilization of QQ proteins with glutaraldehyde (5% v/v) was performed as described previously27 (link). Polyps (10 polyps per dish, 6 dishes) were incubated in artificial seawater for 48 h in presence of QQ proteins prior to microbial DNA isolation for HTP sequencing analysis.
3
Fabrication of Descemet-like PDMS Structures
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The Descemet-like structure was molded in PDMS; therefore, base and curing agent from Sylgard 184 (Dow Corning) were mixed (mass ratio 10:1) and poured on the template. Liquid PDMS was then degassed by vacuum and afterward cured for 48 hours at room temperature. Flat control pieces were casted from polystyrene petri dishes (Greiner Bio-One). As with the master prints, the corresponding silicone imprints differ in both micrometer and nanometer scales.
4
Streptomyces Strain Bioassay on Soy Flour Mannitol Medium
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Unless stated otherwise, bioassays were performed on soy flour mannitol medium (SFM; soy flour 20 g l -1 , mannitol 20 g l -1 , agar 15 g l -1 ) (Kieser et al. 2000) in polystyrene petri dishes (Greiner Bio-One GmbH, Frickenhausen, Germany; 94 × 16 mm with vents; 24 ml medium/dish). The analysed Streptomyces strains are listed in Table 1. Spore suspensions were prepared as described by Kieser et al. (Kieser et al. 2000) . Spore suspensions contained about 5-20 × 10 2 spores per μl. Agar plates were incubated at 28 °C and a relative humidity of about 30-40%.
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