Log In Sign up
The largest database of trusted experimental protocols

Thorimage 3

Manufactured by Thorlabs
Visit Supplier

The ThorImage 3.1 is a high-performance imaging system designed for a variety of scientific and industrial applications. It features a high-resolution sensor, advanced image processing capabilities, and flexible connectivity options. The core function of the ThorImage 3.1 is to capture, process, and output high-quality images.

Automatically generated - may contain errors

Lab products found in correlation

Gaasp photomultiplier tube, by Hamamatsu Photonics (2 mentions) Lsk gr08, by Thorlabs (2 mentions) 0.8 numerical aperture microscope objective, by Nikon (2 mentions) Pfm450e, by Thorlabs (2 mentions) Mai tai hp, by Spectra-Physics (2 mentions)

2 protocols using thorimage 3

1

Two-Photon Calcium Imaging of Neural Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two-photon calcium imaging was performed on a custom-build microscope equipped with a resonant scanning module (LSK-GR08, Thorlabs), GaAsP photomultiplier tube (Hamamatsu), and a 16 × 0.8 numerical aperture microscope objective (Nikon) using ThorImage 3.1 (Thorlabs). We used a Ti-Sapphire laser (Mai Tai HP, Spectra Physics) to excite GCaMP6 at 920 nm. The FOV was 798 μm × 798 μm (512 × 512 pixels) and images were acquired at 30 Hz for single-plane recordings and at 5 Hz for multi-plane recordings. For multi-plane recordings the objective was moved between frames using a piezo objective scanner (PFM450E, Thorlabs). The piezo was allowed to settle for 35 ms in the new z position before the next frame was recorded.
Buetfering C., Zhang Z., Pitsiani M., Smallridge J., Boven E., McElligott S, & Häusser M. (2022). Behaviorally relevant decision coding in primary somatosensory cortex neurons. Nature neuroscience, 25(9), 1225-1236.
+ Open protocol
+ Expand
2

Two-Photon Calcium Imaging of Neural Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two-photon calcium imaging was performed on a custom-build microscope equipped with a resonant scanning module (LSK-GR08, Thorlabs), GaAsP photomultiplier tube (Hamamatsu), and a 16 × 0.8 numerical aperture microscope objective (Nikon) using ThorImage 3.1 (Thorlabs). We used a Ti-Sapphire laser (Mai Tai HP, Spectra Physics) to excite GCaMP6 at 920 nm. The FOV was 798 μm × 798 μm (512 × 512 pixels) and images were acquired at 30 Hz for single-plane recordings and at 5 Hz for multi-plane recordings. For multi-plane recordings the objective was moved between frames using a piezo objective scanner (PFM450E, Thorlabs). The piezo was allowed to settle for 35 ms in the new z position before the next frame was recorded.
Buetfering C., Zhang Z., Pitsiani M., Smallridge J., Boven E., McElligott S, & Häusser M. (2022). Behaviorally relevant decision coding in primary somatosensory cortex neurons. Nature neuroscience, 25(9), 1225-1236.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!