Dawn heleos 2 18

The ultraviolet–visible (UV–Vis) spectra and Fourier transform infrared (FTIR) spectra of LPs and MLPs were measured by the methods of Han et al. [4 (link)]; periodate oxidation analyses were performed with the method described by Hu et al. [15 (link)]; and atomic force microscope (AFM) images were observed according to our previous method [17 (link)].
The M_w distributions of LPs and MLPs were detected using a HPSEC system mainly composed of a model 510 pump (Waters, Milford, MA, USA), a PL aquagel-OH 40 column (8 μm, 7.5 × 300 mm, Agilent), a multi-angle laser light scattering (MALLS) detector (DAWN HELEOS-II 18, Wyatt Technology Co., Santa Barbara, CA, USA), and a differential refractive index (RI) detector (Optilab rEX, Wyatt Technology Co., Santa Barbara, CA, USA). Ammonium acetate solution (0.2 mol/L) was used as the mobile phase at a flow rate of 0.7 mL/min. Five milligrams of samples were dissolved in 2 mL ammonium acetate solution, followed by filtration through a 0.22 μm filter for injection. The injection volume was 20 μL and the column temperature was 30 °C.
After having been kept in a vacuum drier for a week, samples (30–40 mg) were put into a 5 mm NMR tube and dissolved in 1.0 mL dimethyl sulfoxide (DMSO). A Bruker AM 400 MHz spectrometer (Bruker, Rheinstetten, Germany) was used to record ¹H NMR spectrum with the operating frequency of 400.13 MHz at 30 °C.

The molecular weight (MW) distributions of LRPs and LRP-phenol complexes were evaluated by the method of Yi et al. [11 (link)] and using a high-performance size-exclusion chromatograph (HPSEC) equipped with a column of Aglient PL aquagel OH. Samples were dissolved in the mobile phase with a concentration of 0.2 mol/L, and then centrifuged at 13,000 rpm for 5 min. The supernatant was filtered through 0.22 $\mathsf{\mu }$ m film and used for injection. It was eluted by the mobile phase of 0.2 mol/L CH ${}_3$ COOHNH ${}_4$ solution at a flow rate of 0.7 mL/min and column temperature of 30 °C. The peaks were determined using a multi-angle laser-light scattering detector (DAWN HELEOS-II 18, Wyatt Technology Co., Santa Barbara, CA, USA) and refractive index detector (Optilab rEX, Wyatt Technology Co.).