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Fitc conjugated anti cd3 17a2

Manufactured by BioLegend
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The FITC-conjugated anti-CD3 (17A2) is a monoclonal antibody that targets the CD3 antigen on T cells. This antibody is conjugated with the fluorescent dye fluorescein isothiocyanate (FITC), which allows for the detection and identification of CD3-positive cells using flow cytometry.

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Lab products found in correlation

Ficoll paque premium solution, by GE Healthcare (1 mentions) Accuri c6 flow cytometer, by BD (1 mentions) Cd11b m1 70, by BioLegend (1 mentions) Pe conjugated anti il 13 ebio13a, by Thermo Fisher Scientific (1 mentions) Streptavidin bv650, by BioLegend (1 mentions) Apc conjugated anti cd4 gk1.5, by BioLegend (1 mentions) Facsaria 2, by BD (1 mentions) Facscalibur, by BD (1 mentions) Lsrfortessa, by BD (1 mentions)

Close spelling variants of this product

Anti-CD3 FITC (17A2, CD3-FITC (17A2; Anti-CD3 (17A2, FITC-conjugated anti-CD3 CD3 (17A2; FITC conjugated CD3 Anti-CD3-FITC CD3-FITC Anti-CD3

3 protocols using fitc conjugated anti cd3 17a2

1

Hepatic Lymphoid Cell Isolation

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Recipient livers were excised, minced into small fragments and passed through steel mesh with a gauge of 200 μm. The resulting suspension was washed with PBS, and the hepatic lymphoid cells were isolated via discontinuous density gradient centrifugation with Ficoll-Paque PREMIUM solution (GE Healthcare, Little Chalfont, UK). The hepatic lymphoid cells were stained with FITC-conjugated anti-CD3 (17A2; Biolegend, San Diego, CA, USA), PE-conjugated anti-CD4 (RM4-4; Biolegend), APC-conjugated anti-CD8a (53-6.7; Biolegend) and PE-conjugated anti-CD11c (N418; Biolegend). Three-color flow cytometric analysis was conducted using a BD Accuri C6 flow cytometer (BD Biosciences, San Jose, CA, USA).
Yamane K., Anazawa T., Tada S., Fujimoto N., Inoguchi K., Emoto N., Nagai K., Masui T., Okajima H., Takaori K., Sumi S, & Uemoto S. (2020). Mitomycin C treatment improves pancreatic islet graft longevity in intraportal islet transplantation by suppressing proinflammatory response. Scientific Reports, 10, 12086.
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2

Intracellular Cytokine Staining of IL-33-Stimulated Cells

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For co-culture experiments, MLN cells from IL-33-treated mice were stained with PerCp-Cy5.5-conjugated CD90.2 (30-H12) and APC-Cy7-conjugated CD45 (30-F11, Biolegend, San Diego, CA) followed by cell sorting with a FACSAria II (BD Biosciences, San Jose, CA) For intracellular cytokine analyses, after restimulation with phorbol 12-myristate 13-acetate/ionomycin and treatment with Golgi blocker, LP and MLN cells were stained with biotinylated anti–T1/ST-2 (DJ8, MD Biosciences, Oakdale, MN) followed by Streptavidin-BV650, APC-conjugated anti-CD4 (GK1.5), FITC-conjugated anti-CD3 (17A2) and PerCp-Cy5.5-conjugated B220 (RA3–6B2), FCεRI (MAR-1), CD11c (N418), NK1.1 (PK136) and CD11b (M1/70) (Biolegend). Stained cells were fixed and permeabilized for intracellular cytokine staining by using PE-conjugated anti–IL-13 (eBio13A, eBioscience, Waltham, MA). MLN and LP cells were then analyzed with an LSR II (BD Biosciences, San Jose, CA).
Waddell A., Vallance J.E., Hummel A., Alenghat T, & Rosen M.J. (2018). IL-33 induces murine intestinal goblet cell differentiation indirectly via innate lymphoid cell IL-13 secretion. Journal of immunology (Baltimore, Md. : 1950), 202(2), 598-607.
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3

Multiparameter Flow Cytometry Analysis

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For flow cytometry and intracellular cytokine staining, the following mAbs were used: FITC-conjugated anti-CD3 (17A2; BioLegend); phycoerythrin-cyanin 5-conjugated anti-CD4 (RM4-5; BD Bioscience); PE-conjugated anti-IL-17A (TC11-18H10.1; BioLegend); APC-conjugated anti-IFN-γ (XMG1.2; BioLegend); FITC-conjugated anti-CD25 (PC61; BioLegend); PE-conjugated anti-CD69 (H1.2F3; BioLegend); PE- or APC-conjugated anti-Vα2 TCR (B20.1; BD Biosciences). Samples were acquired on FACSCalibur (BD Biosciences) or LSRFortessa (BD Biosciences), and data were analyzed with FlowJo software (TreeStar).
Kang J.O., Lee J.B, & Chang J. (2016). Cholera Toxin Promotes Th17 Cell Differentiation by Modulating Expression of Polarizing Cytokines and the Antigen-Presenting Potential of Dendritic Cells. PLoS ONE, 11(6), e0157015.
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