C2c12 cells

Manufactured by National Collection of Authenticated Cell Cultures

Sourced in China

C2C12 cells are a mouse myoblast cell line derived from the thigh muscle of a C3H mouse. They are commonly used as an in vitro model for studying muscle cell differentiation and function.

Automatically generated - may contain errors

Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Cck 8 kit, by Beyotime (1 mentions) Raw264.7 cells, by National Collection of Authenticated Cell Cultures (1 mentions) Raw264, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

C2C12 cell line (2 citations)

2 protocols using c2c12 cells

Eugenol Cytotoxicity in C2C12 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

C2C12 cells (National Collection of Authenticated Cell Cultures, Shanghai, China) were grown in an incubator with 5% CO₂ at 37°C in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, CA, USA) with 10% fetal bovine serum (FBS, Gibco, CA, USA). The manufacturer’s recommended methods for cell development were followed, and differentiation was achieved by switching the growth media to 2% horse serum, which was supplemented for 4 days.
Cytotoxicity was measured at different doses using a CCK-8 kit (Beyotime, Shanghai, China). The cells were seeded (4 × 10³ cells per well) in 96-well plates. After 12 h of growth, the medium was replaced and treated with various doses of eugenol (25, 50, 100, 200, and 400 μM) dissolved in DMSO (0.5%). The absorbance value of cells at 480 nm was read to calculate the cell cytotoxicity results.

Jiang Y., Feng C., Shi Y., Kou X, & Le G. (2022). Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake. Frontiers in Nutrition, 9, 1039753.

+ Open protocol

+ Expand

Indirect Co-culture of RAW264.7 and C2C12 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

RAW264.7 cells and C2C12 cells were obtained from National Collection of Authenticated Cell Cultures (Shanghai, China). RAW264.7 cells were cultured in Dulbecco’s modified Eagle’s Medium (DMEM; 25 mM glucose; Gibco, USA) containing 10% FBS, 1% penicillin/streptomycin, and with 20 ng/mL RANKL at 37 °C in a humidified atmosphere of 95% air and 5% CO₂. On the third or fifth day, the cells were cultured with RANKL-free and glucose-free medium for 12h. Then, the medium was collected for indirect co-culturing with C2C12 cells. The medium from RAW264.7 cells without RANKL induction was also collected as control.
C2C12 myoblasts were cultured in DMEM supplemented with 10% FBS (Gibco, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified atmosphere of 95% air and 5% CO2. On the second day, the medium was changed to DMEM with 2% horse serum (Gibco, USA) to induce differentiation for an additional 4 days. The medium was replaced every two days.
The differentiated C2C12 myoblasts (10⁴ cells/well) were cultured with the conditioned mediums (CMs) containing 40% medium collected from RAW264.7 cells (non-RANKL-treated, CCM; RANKL-treated for 3 days and 5 days, CM3 and CM5) and 60 mM of glucose (n = 6) for 24 h in a 24-well plate.

Li X., Sun F., Lu J., Zhang J., Wang J., Zhu H., Gu M, & Ma J. (2021). Osteoclasts May Affect Glucose Uptake-Related Insulin Resistance by Secreting Resistin. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 14, 3461-3470.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!