Streptomycin double antibody

All the ultra-pure water used in the experiments was purified by a Mili-Q A10 filtration system (Milipore, Billerica, MA, United States). Phosphate-buffered saline (PBS) was obtained from Solarbio (Beijing, China). Low glucose Dulbecco’s Modified Eagle’s Medium (LGDMEM), fetal bovine serum (FBS), and streptomycin double antibody were obtained from Gibco (Grand Island, NY, United States). Hematoxylin and Eosin (H&E) stain dye solution was purchased from Thermo Fisher Scientific Co., Ltd. (Shanghai, China). Calcein-AM-PI staining kit were obtained from Bestbio (Nanjing China). Rhodamine-phalloidin was obtained from Solarbio (Beijing, China). Opioid receptor antibody, the voltage-gated Na (+) channel subtype Nav1.7 antibody, and the P substance receptor antibody were purchased from Abcam (MA, United States). Goat antirabbit IgG were purchased from the Life Technologies (United States).

Ti6Al4V powder was obtained from AK Medical (Beijing, China). Poloxamer 407 was supplied by Bayee Chemical Co., Ltd. (Hangzhou, China). Rabbit endothelial progenitor cells (BFN60810500) was purchased from ATCC (Manassas, VA, USA) and Rabbit BMSCs (RBXMX-01001) was supplied by Cyagen Biosciences (Guangzhou, China). Low Glucose Dulbecco’s Modified Eagle’s Medium (LG-DMEM), fetal bovine serum (FBS), and streptomycin double antibody were supplied by Gibco (Grand Island, NY, USA). Cell Counting Kit‑8 (CCK‑8) assay was supplied by (Dojindo, Shanghai, China) and Calcein-AM/PI Kits was purchased from Beyotime Co., Ltd. (Shanghai, China). Microfil (MV122, Flow Tech, Carver, MA, USA) was used for microangiography. RNA and the Revert Aid First Strand cDNA Synthesis Kit, and SYBR Premix Ex TaqTM kit were supplied by TaKaRa (Dalian, China). Trizol Reagent was obtained from Invitrogen (CA, USA).