Anti histone h3

For Western blotting, equal amounts of protein extracts from whole insects were separated by SDS-PAGE and transferred onto PVDF. The membranes were blocked, incubated with the corresponding primary antibody. The primary antibodies for western blots were diluted with 5% skim milk in 0.1% Tween/TBS for anti-TcAK1 (1:1,000), anti-TcAK2 (1:2,000), anti-TcFOXO (1:1,000), anti-TcAMPKα (1:1,000), anti-α-tubulin (1:5,000) (Proteintech Group, Inc., Chicago, IL, USA), anti-Histone H3 (1:5,000) (Beyotime, Shanghai, China), Mouse Anti-AMPK alpha 1 + AMPK alpha 2 antibody (1:1,000), Recombinant Anti-GST antibody (1:5,000) (Abcam, Cambridge, MA, USA). The specificity of the TcAK1, TcAK2, TcFOXO and TcAMPKα antibodies was verified in our previous studies [29 (link), 58 (link), 59 (link)] The membranes were washed and incubated with the corresponding secondary antibody such as Goat anti-mouse IgG/HRP (1:10,000) (Solarbio, Beijing, China) and Goat anti-rabbit IgG/HRP antibody (1:10,000) (Solarbio, Beijing, China). The blot signals were detected using Tanon High-sign ECL Western Blotting kit (Tanon, Shanghai, China) with a ChemiDoc Touch Imaging System (Bio-Rad, Hercules, CA, USA) as described previously [29 (link)].

Western blot analysis was performed as previously reported (Song et al., 2021 (link)). Briefly, protein samples were prepared from neonatal rat cardiac cells and mice hearts, and were denatured and separated on 10% polyacrylamide gels by SDS-PAGE, and then transferred to polyvinylidene difluoride (PVDF) membranes. Target proteins were detected using standard procedures. Band intensities were normalized to housekeeping gene Histone H3 or β-actin. The following primary antibodies were used in this study: anti-Angpt4 (Invitrogen), anti-β-actin (Abclonal), anti-pERK (Cell Signaling Technology), and anti-Histone H3 (Beyotime).

Western blot analysis was performed as previously reported (Song et al., 2021 (link)). Briefly, protein samples were prepared from neonatal rat cardiac cells and mice hearts, and were denatured and separated on 10% polyacrylamide gels by SDS-PAGE, and then transferred to polyvinylidene difluoride (PVDF) membranes. Target proteins were detected using standard procedures. Band intensities were normalized to housekeeping gene Histone H3 or β-actin. The following primary antibodies were used in this study: anti-Angpt4 (Invitrogen), anti-β-actin (Abclonal), anti-pERK (Cell Signaling Technology), and anti-Histone H3 (Beyotime).