Rat glomerular mesangial cell line (HBZY-1) was obtained from Boster Biological Technology Co., Ltd. (Wuhan, China) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) at 37°C. The TLR4 overexpression plasmid pcDNA3.1-TLR4 and empty pcDNA3.1 vector were purchased from GenePharma (Shanghai, China). Transfection of pcDNA3.1-TLR4 and pcDNA3.1 vector was performed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, United States) according to the manufacturer’s instructions. At 48 h after transfection, western blot was performed to determine transfection efficiency.
Pcdna3.1 tlr4
Manufactured by GenePharma
Sourced in China
PcDNA3.1-TLR4 is a plasmid vector that contains the Toll-like receptor 4 (TLR4) gene. It is designed for the expression of the TLR4 protein in mammalian cell lines.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pcdna3.1 tlr4
1
TLR4 Overexpression in Rat Mesangial Cells
2
Intracerebral Hemorrhage Rat Model
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miR-negative control (NC) mimic, miR-30e-5p mimic, pcDNA3.1, and pcDNA3.1-TLR4 were purchased from Shanghai GenePharma Co., Ltd. Rat is an ideal pathophysiological disease model that is widely used (23 (link),24 (link)). The ICH model in rats was established by IC injection of collagenase type IV (0.23 U in 1 µl sterile saline; Beijing Solarbio Science & Technology Co., Ltd.) into the right striatum, as previously described (25 (link)). For the rats in the ICH group (n=11), miR-NC mimic + pcDNA3.1 was injected into the right lateral ventricle of rats 3 days prior to the establishment of ICH model.
Rats in the sham group (n=11) were injected with 1 µl sterile saline. For rats in the remaining groups (ICH + miR-30e-5p mimic + pcDNA3.1 and ICH + miR-30e-5p mimic + pcDNA3.1-TLR4; both n=11), miR-30e-5p mimic + pcDNA3.1 or miR-30e-5p mimic + pcDNA3.1-TLR4 was injected into the right lateral ventricle of rats 3 days prior to establishment of ICH model (26 (link)).
Rats in the sham group (n=11) were injected with 1 µl sterile saline. For rats in the remaining groups (ICH + miR-30e-5p mimic + pcDNA3.1 and ICH + miR-30e-5p mimic + pcDNA3.1-TLR4; both n=11), miR-30e-5p mimic + pcDNA3.1 or miR-30e-5p mimic + pcDNA3.1-TLR4 was injected into the right lateral ventricle of rats 3 days prior to establishment of ICH model (26 (link)).
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