After 24 h of co-culture, the SH-SY5Y cells were detached for the apoptosis assay using the PI/Annexin V stain (BD Biosciences, CA, USA) method, according to the manufacturer’s protocol. The cells in each group were stained with PI dye (10 μl) and Annexin V dye (5 μl) for 15 min at room temperature. Then, the results were analyzed with Accur C6 Plus flow cytometry (BD Biosciences, CA, USA). The apoptotic index was calculated as follows: Annexin V+/PI− cells/total cells × 100% [22 ].
Pi annexin 5 stain
Manufactured by BD
Sourced in United States
PI/Annexin V stain is a fluorescent labeling reagent used in flow cytometry and microscopy applications to assess cell viability and apoptosis. It consists of propidium iodide (PI) and Annexin V, which bind to different cellular components to differentiate between viable, early apoptotic, and late apoptotic/necrotic cells.
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Lab products found in correlation
3 protocols using pi annexin 5 stain
1
Apoptosis Assay: SH-SY5Y Cell Co-culture
2
Quantifying Neuronal Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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After 24 h of co-culture, neurons were detached for the apoptosis assay using the PI/Annexin V stain (BD Biosciences, CA, USA) method, according to the manufacturer's protocol. The cells in each group were stained with PI dye (10 μL) and Annexin V dye (5 μL) for 15 min at room temperature. Then, the results were analyzed with Accur C6 Plus ow cytometry (BD Biosciences, CA, USA). The apoptotic index was calculated as follows: Annexin V+/PI-cells/total cells × 100% [22] .
3
Quantifying Neuronal Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 24 h of co-culture, neurons were detached for the apoptosis assay using the PI/Annexin V stain (BD Biosciences, CA, USA) method, according to the manufacturer's protocol. The cells in each group were stained with PI dye (10 μL) and Annexin V dye (5 μL) for 15 min at room temperature. Then, the results were analyzed with Accur C6 Plus ow cytometry (BD Biosciences, CA, USA). The apoptotic index was calculated as follows: Annexin V+/PI-cells/total cells × 100% [22] .
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