Hlpc grademethanol meoh

Manufactured by Thermo Fisher Scientific

HPLC-grade methanol (MeOH) is a highly purified form of methanol specifically designed for use in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile, and polar organic solvent with a high purity level, typically exceeding 99.9%. HPLC-grade methanol is used as a mobile phase component in HPLC systems to facilitate the separation, identification, and quantification of various analytes in complex samples.

Automatically generated - may contain errors

Lab products found in correlation

Formic acid, by Merck Group (3 mentions) Cell free amino acid mixture 15n, by Merck Group (2 mentions) Lc ms grade acetonitrile, by Thermo Fisher Scientific (1 mentions) Glucose d2, by Merck Group (1 mentions) Milli q water, by Merck Group (1 mentions) Milli q plus, by Merck Group (1 mentions)

2 protocols using hlpc grademethanol meoh

Targeted Metabolomics Using Nano-DESI

Check if the same lab product or an alternative is used in the 5 most similar protocols

Solvents were HLPC-grade
methanol (MeOH) (Fisher Scientific), water from Milli-Q Plus, LC/MS-grade
acetonitrile (Fisher Chemical), and formic acid (Merck). The standards
used for quantitation were Cell Free Amino Acid Mixture-¹⁵N (Sigma-Aldrich), glucose-d₂, γ-aminobutyric
acid-d₂ (GABA-d₂), acetylcholine-d₉, oleic acid-d₉ (FA 18:1-d₉),
LPC 19:0, PC 11:0/11:0. Solvents for PA nano-DESI analysis were MeOH/H₂O (9:1) with 0.1% formic acid or ACN/MeOH (9:1) with 0.1%
formic acid (Table S1).

Marques C., Friedrich F., Liu L., Castoldi F., Pietrocola F, & Lanekoff I. (2023). Global and Spatial Metabolomics of Individual Cells Using a Tapered Pneumatically Assisted nano-DESI Probe. Journal of the American Society for Mass Spectrometry, 34(11), 2518-2524.

+ Open protocol

+ Expand

Metabolite Quantification in Insulin-Secreting Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Solvents for extraction
were HLPC-grade methanol (MeOH) (Fisher Scientific), Milli-Q water
(H₂O) from Milli-Q Plus, and formic acid (Merck). For quantification
the internal standards oleic acid-d₉ (FA 18:1-d₉), LPC 19:0, PC 11:0/11:0, GABA-d₂, Acetylcholine-d₉, Glucose-d₂, and Cell Free Amino Acid Mixture-¹⁵N (Sigma-Aldrich) were prepared in MeOH:H₂O (9:1)
with 0.1% formic acid (Tables S1 and S2). INS-1 cells were exposed to a solution contained 138 mM NaCl,
5.6 mM KCl, 1.2 mM MgCl₂, 2.6 mM CaCl₂, and
1, 3, or 20 mM d-glucose at pH 7.4 and room temperature.

Marques C., Liu L., Duncan K.D, & Lanekoff I. (2022). A Direct Infusion Probe for Rapid Metabolomics of Low-Volume Samples. Analytical Chemistry, 94(37), 12875-12883.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!