Pristine psnpls pp 008 10

Pristine PSNPLs (PP-008-10) and yellow fluorophore-conjugated PSNPLs (y-PSNPLs) (FP-00552-2) were purchased from Spherotech (Chicago, IL, USA). The pristine form of polystyrene (PS) particles was used for all the experiments carried out except for those in which the fluorescent marker was required, such as the visualization and the quantification of PS particles internalization by confocal microscopy and flow cytometry, respectively. PSNPLs used for the assays were characterized using Zetasizer and transmission electron microscopy (TEM) methodologies. To that purpose, the obtained dispersions were diluted to a final concentration of 100 μg/mL in distilled water. The hydrodynamic size and the Z-potential values for PSNPLs and y-PSNPLs dispersions were determined in a Malvern Zetasizer Nano ZS zen3600 device (Malvern, UK). All the parameters for each sample were measured in triplicates. TEM grids were dipped into PSNPLs and y-PSNPLs dispersions and visualized on a JEOL JEM-1400 instrument (JEOL LTD, Tokyo, Japan). To determine the mean size, 100 randomly selected PSNPLs were measured using an Image J software with the Fiji extension.