For immunohistochemical analysis, formalin-fixed paraffin-embedded blocks of the mice’s liver tissue were cut into 2-µm sections. After deparaffinization and rehydration, sections were heated in a citrate buffer (10 mM, pH 6, Thermo Fisher Scientific, Waltham, MA, USA) in a microwave for 30 min to retrieve the antigens. Endogenous peroxidase activity was blocked with a 3% hydrogen peroxide (UltraVision Hydrogen Peroxide Block; Thermo Fisher Scientific) for 10 min. The sections were then incubated with a PTEN antibody (Santa Cruz, Santa Cruz, CA, USA) for 1 h at room temperature. PTEN immunoreactivity in sections was visualized using HRP polymer (UltraVision Quanto Detection System; Thermo Fisher Scientific) and DAB chromogen (DAB Peroxidase Substrate Kit; Vector Laboratories, Burlingame, CA, USA). The sections were counterstained with Mayer’s hematoxylin (ScyTek Laboratories, Logan, UT, USA), dehydrated and then mounted using a mounting medium.
Pten antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States, China
The PTEN antibody is a reagent used in research applications to detect and study the Phosphatase and Tensin Homolog (PTEN) protein. PTEN is a tumor suppressor protein that plays a role in regulating cell growth and division. The antibody can be used for techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to identify and characterize the PTEN protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Citrate buffer, by Thermo Fisher Scientific (1 mentions)
Mayer s hematoxylin, by ScyTek Laboratories (1 mentions)
Dab peroxidase substrate kit, by Vector Laboratories (1 mentions)
Ultravision hydrogen peroxide block, by Thermo Fisher Scientific (1 mentions)
Ultravision quanto detection system, by Thermo Fisher Scientific (1 mentions)
Cd45 fitc, by BioLegend (1 mentions)
Cd90 fitc, by BioLegend (1 mentions)
Cd34 fitc, by BioLegend (1 mentions)
Cd105 fitc, by BioLegend (1 mentions)
Cd73 fitc, by BioLegend (1 mentions)
Anti p53 antibody, by Merck Group (1 mentions)
Hla abc, by BD (1 mentions)
Fitc hla a b c antibody, by BioLegend (1 mentions)
Percp cy5.5 cd146, by BioLegend (1 mentions)
Pe human ng2 mcsp, by BioLegend (1 mentions)
Bv421 ki67, by BioLegend (1 mentions)
Fitc anti s100, by Abcam (1 mentions)
Horseradish peroxidase conjugated goat anti mouse igg, by ZSGB-BIO (1 mentions)
Dab chromogenic reagent kit, by ZSGB-BIO (1 mentions)
Tcs sp2 aobs, by Leica (1 mentions)
4 protocols using pten antibody
1
Immunohistochemical Analysis of PTEN in Murine Liver
2
Multiparametric Flow Cytometry and Immunohistochemistry
3
Immunohistochemical Analysis of PTEN
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The deparaffinized tissue sections were performed to antigen retrieval in 0.01% Sodium Citrate buffer at 95 to 98°C for 15 min and were blocked with 3% peroxide-methanol at room temperature for 10min for endogenous peroxidase ablation. After that, the tissue sections were incubated with 5% normal goat serum at room temperature for 20 min, and then incubated overnight at 4°C with a mouse monoclonal PTEN antibody (1:150, Santa Cruz Biotechnology, Inc., SC-393186, Santa Cruz, CA). Then the tissue sections were rinsed in 0.01 M PBS (pH 7.4) and incubated with horseradish peroxidase-conjugated goat antimouse IgG (1:200, ZSGB-BIO, ZB-2305, Beijing, China) for 2 h at room temperature. The tissues were washed, and immunoreactivity was visualized using the DAB chromogenic reagent kit (ZSGB-BIO, ZLI-9018, Beijing, China) and hematoxylin restaining. The sections were mounted after the final rinse. The specificities of the immunostaining were verified by omitting the primary antibody from incubation.
4
PTEN Expression Imaging in BMECs
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BMECs transfected with mimics, NC (negative control), and inhibitors were cultured on glass-bottomed cell culture dishes (NEST, 801002); then, the liquid was removed gently, and the cells were rinsed twice with TBSTx (TBS supplemented with 0.1% TritonX-100). The cells were fixed in ice-cold 4% paraformaldehyde for 10 min and then washed with TBSTx 3×5 min. The cells were incubated with TBSTx containing 5% BSA (bovine serum albumin) (TBSTx-5% BSA) and then incubated with the PTEN antibody (Santa Cruz Biotechnology) diluted 1:50 with TBSTx-5% BSA, and finally incubated with 1:200 FITC-conjugated goat anti-rabbit IgG secondary antibodies (ZSGB-BIO, Beijing, China); all antibodies were incubated with the cells for 1 h at 37°C, and all wash steps involved TBSTx 3×5 min. The plates were stained with propidium iodide (PI) (Roche, Florence, SC, USA). Images were captured using a confocal laser scanning microscope (Leica TCS SP2 AOBS, Germany).
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