An Olympus spinning disk confocal system (Olympus SD OSR) (UAPON 100XOTIRF/1.49 oil objective) was used to image fixed samples and the microscope was operated by Metamorph software. Whenever there was live imaging, a cellVivo incubation module was used to maintain cells at 37°C and 5% CO2. For FISH and nucleoid-cGAS overlap images, imaging was performed using a Plan-Apochromat ×63/1.4 NA oil objective on an inverted Zeiss spinning disk microscope using 405, 488, 561 and 633 nm laser lines.
Uapon 100xotirf 1.49 oil objective
Manufactured by Olympus
The UAPON 100XOTIRF/1.49 oil objective is a high-performance lens designed for use in total internal reflection fluorescence (TIRF) microscopy. The objective has a magnification of 100X and a numerical aperture of 1.49, providing high-resolution imaging capabilities. The objective is specifically optimized for TIRF applications, enabling the efficient excitation and detection of fluorescent samples close to the coverslip surface.
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Lab products found in correlation
3 protocols using uapon 100xotirf 1.49 oil objective
1
Imaging Cellular Structures with Spinning Disk Confocal
2
Imaging of Organelle Dynamics in Diverse Cell Lines
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Images for MEFs, DRP1 mutant fibroblasts and their wild-type control were acquired with a Leica TSC SP8 confocal microscope fitted with a 63×/1.40 oil objective using the optimal resolution for the wavelength (determined using the Leica software). Images from MYH14 cells and their control were taken with an Olympus spinning disc confocal system (Olympus SD-OSR) (UAPON 100XOTIRF/1·49 oil objective) operated by Metamorph software. The SD-OSR was equipped with a cellVivo incubation module to maintain cells at 37ºC and 5% CO2 during live cell imaging.
3
Multimodal Imaging of Cellular Organelles
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Images for MEFs, DRP1 mutant fibroblasts and their wild-type control were acquired with a Leica TSC SP8 confocal microscope fitted with a 63x/1.40 oil objective using the optimal resolution for the wavelength (determined using the Leica software). Images from MYH14 cells and their control were taken with an Olympus spinning disc confocal system (Olympus SD-OSR) (UAPON 100XOTIRF/1•49 oil objective) operated by Metamorph software. The SD-OSR was equipped with a cellVivo incubation module to maintain cells at 37ºC and 5% CO2 during live cell imaging.
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