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Cell surface markers

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Cell surface markers are lab equipment used to identify and characterize cells. They are specific molecules found on the surface of cells that can be detected and measured using various analytical techniques.

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Lab products found in correlation

Brilliant violet 421, by BioLegend (2 mentions) Live dead stain, by Thermo Fisher Scientific (2 mentions) Alexa flour 647, by Thermo Fisher Scientific (2 mentions) Facsaria 2, by BD (2 mentions)

Close spelling variants of this product

Monoclonal antibodies against cell surface markers

2 protocols using cell surface markers

1

Single-cell sorting of HIV-specific B cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell sorting of antigen-specific B cells was performed as previously described (Morris et al., 2011 (link); Wu et al., 2010 (link)) with the following modifications. Biotinylated CAP45 cyclic peptides comprising gp120 residues 157 – 196 (Genscript) were labeled with either Brilliant Violet 421 (BV421, Biolegend), or AlexaFlour 647 (AF647, Life Technologies), conjugated to streptavidin. CAP228 PBMCs were stained with a variety of cell surface markers (BD Biosciences) as well as live/dead stain (Invitrogen), and antigen-specific memory B cells were sorted as CD3-, CD14-, CD16-, CD19+, IgD- and CAP45 peptide K169+ E169- into 96-well PCR plates containing lysis buffer, using a BD FACS Aria II (BD Biosciences). In a second sort, the CAP228 autologous transmitted/founder V1V2 domain was scaffolded onto PDB ID: 1FD6 (McLellan et al., 2011 (link)), and labeled with BV421 as an addifigtional positive bait. Data were analyzed using FlowJo (Tree Star).
van Eeden C., Kurt Wibmer C., Scheepers C., Richardson S.I., Nonyane M., Lambson B., Mkhize N.N., Vijayakumar B., Sheng Z., Stanfield-Oakley S., Bhiman J.N., Bekker V., Hermanus T., Mabvakure B., Ismail A., Moody M.A., Wiehe K., Garrett N., Karim S.A., Dirr H., Fernandes M.A., Sayed Y., Shapiro L., Ferrari G., Haynes B.F., Moore P.L, & Morris L. (2018). V2-Directed Vaccine-like Antibodies from HIV-1 Infection Identify an Additional K169-Binding Light Chain Motif with Broad ADCC Activity. Cell reports, 25(11), 3123-3135.e6.
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2

Single-cell sorting of HIV-specific B cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell sorting of antigen-specific B cells was performed as previously described (Morris et al., 2011 (link); Wu et al., 2010 (link)) with the following modifications. Biotinylated CAP45 cyclic peptides comprising gp120 residues 157 – 196 (Genscript) were labeled with either Brilliant Violet 421 (BV421, Biolegend), or AlexaFlour 647 (AF647, Life Technologies), conjugated to streptavidin. CAP228 PBMCs were stained with a variety of cell surface markers (BD Biosciences) as well as live/dead stain (Invitrogen), and antigen-specific memory B cells were sorted as CD3-, CD14-, CD16-, CD19+, IgD- and CAP45 peptide K169+ E169- into 96-well PCR plates containing lysis buffer, using a BD FACS Aria II (BD Biosciences). In a second sort, the CAP228 autologous transmitted/founder V1V2 domain was scaffolded onto PDB ID: 1FD6 (McLellan et al., 2011 (link)), and labeled with BV421 as an addifigtional positive bait. Data were analyzed using FlowJo (Tree Star).
van Eeden C., Kurt Wibmer C., Scheepers C., Richardson S.I., Nonyane M., Lambson B., Mkhize N.N., Vijayakumar B., Sheng Z., Stanfield-Oakley S., Bhiman J.N., Bekker V., Hermanus T., Mabvakure B., Ismail A., Moody M.A., Wiehe K., Garrett N., Karim S.A., Dirr H., Fernandes M.A., Sayed Y., Shapiro L., Ferrari G., Haynes B.F., Moore P.L, & Morris L. (2018). V2-Directed Vaccine-like Antibodies from HIV-1 Infection Identify an Additional K169-Binding Light Chain Motif with Broad ADCC Activity. Cell reports, 25(11), 3123-3135.e6.
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