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2 protocols using cannabigerol

1

Cannabinoid Analytical Procedures

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Chloroform, n-hexane, methanol and ethanol were purchased from Merck (Darmstadt, Germany). N° 1 filters (70 mm diameter) were used (Whatmann, Maidstone, England). N, O-Bis(-trimethylsilyl)trifluoroacetamide with trimethylchlorosilane (BSTFA:TMCS, 99:1, v/v), N-methyl-N-(trimethylsilyl)tri-fluoroacetamide:trimethylchlorosilane (MSTFA:TMCS, 99:1, v/v) and (trimethylsilyl)diazomethane solution were supplied by Sigma Aldrich (Germany). Certified phytocannabinoid mixture 1 (1 mg/mL in acetonitrile; containing cannabidiolic acid (CBDA), cannabigerolic acid (CBGA), cannabigerol (CBG), can-nabidiol (CBD), tetrahydrocannabinoid acid A (THCA), Δ9-tetrahydrocannabinol (Δ9-THC), Δ8-tetrahydrocannabinol (Δ8-THC) and cannabihromene (CBC) at 100 μg/mL of each compound), (−)-Δ9-THC-D3 (THCd3, 0.1 mg/mL in methanol), THCA (1.0 mg/mL in methanol) and Δ9-THC (0.1 mg/mL in methanol) were purchased from LGC Standards S.r.L. (Milano, Italy). Millipore membrane filters (0.45 μm and 0.20 μm) was supplied by Merck (Germany).
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2

Analytical Standards for Cannabis Research

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Alkane standard solution (C8-C20, ~40 mg/L each, in Hexane), fatty acid methyl ester (FAME) mix (C8-C24), Mueller-Hinton (MH) broth, bisabolol (≥93%), caryophyllene (≥98%), myrcene (≥98%), linalool (≥99%), tridecanoic acid (≥98%), hydrochloric acid (≥37%), and glucose (≥99%) were acquired from Merck KGaA (Darmstadt, Germany). Hexane (≥95%), ethanol (≥99%), methanol (≥99%), acetonitrile (≥99%), and water (≥99%) were from VWR Chemicals (Ismaning, Germany). Caryophyllene oxide (≥95%) was bought from Thermo Scientific (Olching, Germany), whereas humulene (≥97%) was purchased from Biomol (Hamburg, Germany). The certified reference materials of 13 cannabinoids containing cannabidivarinic acid (CBDVA), cannabidivarin (CBDV), cannabigerolic acid (CBGA), cannabigerol (CBG), cannabidiol (CBD), tetrahydrocannabivarin (THCV), cannabinol (CBN), ∆ 9 -tetrahydrocannabinol (∆ 9 -tTHC), ∆ 8tetrahydrocannabinol (∆ 8 -THC), cannabicyclol (CBL), cannabichromene (CBC), and ∆ 9tetrahydrocannabinolic acid (∆ 9 -THCA) was supplied from LGC Standards (Kielpin, Poland). The dried Cannabis sativa (hemp) flowers (cv. Futura 75) were provided by Hempartis GmbH (Malsch, Germany).
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