Optical microscopy with polarized light was used to evaluate the morphology and crystal quality of the resulting MBQ-167 crystals. Optical micrographs were collected within a Nikon Polarizing Microscope Eclipse LV100N POL, equipped with a Nikon DS-Fi2 camera and NIS Elements BR software (v. 4.30.01). Using a small amount of paratone oil the single crystals for each form were mounted in MiTeGen micro loops. Crystal structures were determined from diffraction data collected using a Rigaku XtaLAB SuperNova single microfocus Cu-Kα radiation (λ = 1.5417 Å) source equipped with a HyPix3000 X-ray detector in transmission mode (50 kV and 1 mA). An Oxford Cryosystems Cryostream 800 cooler was used to collect the data at 300 and 100 K. Data was analyzed using the CrysAlisPRO software (v. 1.171.39.46). All crystal structures of MBQ-167 were solved by direct methods using SHELXS. The refinement was made using full-matrix least squares on F2 within the Olex2 software (v. 1.2). (Dolomanov et al., 2009 (link)) All non-hydrogen atoms were anisotropically refined.
Ds fi2 camera
Manufactured by National Instruments
The DS-Fi2 camera is a digital imaging device designed for scientific and industrial applications. It features a high-resolution sensor, advanced image processing capabilities, and flexible connectivity options. The camera's core function is to capture and transfer high-quality images for various analytical and documentation purposes.
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Lab products found in correlation
4 protocols using ds fi2 camera
1
Structural Analysis of MBQ-167 Crystals
2
Microscopic Imaging of Cell Cultures
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Daily observations were performed using an inverted phase-contrast microscope (Nikon Eclipse, TS100). Images were acquired with a Nikon DS-Fi2 camera and NIS Elements version 4.0 Imaging System. Images were assembled using Adobe Photoshop.
3
Immunohistochemical Analysis of CLIC1, CLIC4 and CA125
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The paraffin embedded TMA slides were deparaffinized by washing in xylene followed by rehydration. Antigens were retrieved by treating the slides with citrate buffer (pH 6.0) and endogenous peroxidase activity was quenched by 3% H2O2. Subsequently the TMAs were incubated with 3% BSA to prevent nonspecific antibody binding. After blocking, the TMAs were incubated with monoclonal antibodies against CLIC1 (1:450, ab77214), CLIC4 (1:100, ab183043) and CA125 (1:250, ab110640) overnight at 4 °C, followed by further incubation with HRP labeled anti-rabbit, anti-mouse or anti-goat secondary antibody as appropriate. TMAs were then treated with di-aminobenzidine, counter stained with Mayer’s hematoxylin, dehydrated and mounted in crystal mount medium. The TMA slides were then subjected to blind, unbiased analyses by co-author RD. The tissue staining was scored as 0, 1, 2 or 3 based on their intensities. 0 was considered negative while 1, 2 and 3 were considered positive. Scoring was performed by an expert pathologist in a blinded manner (RD). Images of tissue cores were obtained using a Nikon DS-Fi2 camera and were analyzed using NIS Elements software (version 4.13).
4
Thermal Analysis of Fatty Acid-Polyethylene Glycol Mixtures
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