Multiskan spectrum microplate uv vis reader

The turbidity of control (0.1 MPa) and pressure-treated mealworm protein solutions (10% w/v) was measured by spectrophotometry at 595 nm (Thermo Labsystems Multiskan Spectrum Microplate UV–vis reader, Thermo Fisher Scientific, Waltham, MA, USA). First, protein solutions were diluted 1:16 with deionized water. A volume of 200 µL of each protein solution diluted 1:16 was loaded into transparent 96-well microplates (Greiner Bio-One, Kremsmuünster, Austria). The turbidity measurements were reported as optical density at 595 nm (OD₅₉₅), and deionized water was used as a blank sample. Turbidity measurements of all samples and blanks were performed in triplicate, and one measurement was taken per replicate.