Dxc 800 analyzer

Venous blood was drawn from all individuals after a 12 h overnight fast prior to coronary angiography and was centrifuged immediately at 3000 r/min and 4°C for 10 min. Plasma specimens were then collected and stored at −80°C until further analysis. All specimens were analyzed at the same time to control for testing variability. Plasma AAT and high-sensitivity C-reactive protein (hsCRP) levels were measured by immunoturbidimetry[10 (link)] using an IMMAGE 800 immunochemistry system (Beckman Coulter, USA). The AAT reagent (447740, Beckman Coulter, USA) and hsCRP reagent (474630, Beckman Coulter, USA) were used according to the manufacturer's instructions. Total cholesterol and triglycerides were assayed by routine enzymatic methods (GPO-PAP) using a Beckman DxC800 analyzer (Fullerton, USA). High-density lipoprotein cholesterol (HDL-C) level was measured using a chemical modification and selective melting kit (Kyowa Medex, Tokyo, Japan). The low-density lipoprotein cholesterol (LDL-C) concentration was calculated using the Friedeward equation.[11 (link)] The intra- and inter-assay coefficients of variation for plasma AAT level ranged from 2.1% to 3.1%, and 2.8% to 3.3%, respectively. The AAT level reference range was 88–174 mg/dl.

Blood fasting glucose was checked from tail-vein blood using an automatic glucose monitor (One Touch). Plasma triglycerides, and total cholesterol and liver triglyceride were measured with colorimetric assay kits (Wako, Osaka, Japan). Total liver lipids were extracted with chloroform-methanol (2:1, v/v) mixture according to the Folch method [12 (link)]. Plasma insulin levels were measured with a commercial insulin assay ELISA kit (ALPCO Diagnostics, Salem, NH, USA). Serum levels of alanine aminotransferase (ALT) were determined using a Beckman DXC 800 analyzer (Brea, CA, USA).