Animals were euthanized and perfused as described above. A circular 4 mm biopsy of the scar or corresponding sham-operated myocardium was used for imaging. After collection, tissues were briefly washed in wash buffer (0.2 M Hepes buffer solution, pH 7.40, Electron Microscopy Sciences, #11494) and then immediately fixed in 3% glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.4 (Electron Microscopy Sciences, #16538–15) for 2 h at room temperature. Samples were then washed twice in 0.1 M cacodylate buffer pH 7.4 and dehydrated stepwise in a graded ethanol series. Samples were critical point dried in 100% ethanol with a critical point drying machine (CPD300, Leica, Austria) and afterwards glued onto leit-tabs (Plano, Germany) mounted on aluminum stubs (Plano, Germany) and were sputter-coated with a 10-nm-thin gold layer (ACD600, Leica, Austria). SEM images were acquired with a JEOL JSM-IT200 SEM (JEOL, Japan) at 10 kV with an SE detector.
Jsm it200 sem
Manufactured by JEOL
Sourced in Japan
The JEOL JSM-IT200 is a scanning electron microscope (SEM) that provides high-resolution imaging and analysis capabilities. The core function of the JSM-IT200 is to generate and detect electron beams to create detailed images of samples at the micro- and nanoscale levels.
Automatically generated - may contain errors
Lab products found in correlation
Cpd300, by Leica (2 mentions)
Acd600, by Leica (2 mentions)
Sodium cacodylate buffer, by Electron Microscopy Sciences (1 mentions)
Dc3 420t digital microscope, by Xiaomi (1 mentions)
Mi note 10 lite cameras, by Xiaomi (1 mentions)
Am b 10, by Optika (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Dsc 4000, by PerkinElmer (1 mentions)
Dtg 60h, by Shimadzu (1 mentions)
Jsm it200, by JEOL (1 mentions)
Sc7620, by Quorum Technologies (1 mentions)
6 protocols using jsm it200 sem
1
Scanning Electron Microscopy of Cardiac Tissue
2
Morphological Characterization of Seeds
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Mature seeds (20 seeds) were examined using a stereomicroscope (National DC3-420T Digital Microscope) and photographed using Xiaomi Mi Note 10 Lite cameras. Seed color, length, and width, as well as hilum position, were studied. For SEM investigation, the mature seeds were mounted onto SEM stubs, coated with gold, and examined and photographed using a JEOL JSM-IT200 SEM. The terminology was followed as given by Barthlott [33 (link)] and Stearn [34 ]. SEM photographs were carried out in the Electron Microscope Unit, Faculty of Science, Alexandria University.
3
Pollen Grain Morphological Analysis
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Flowers were fixed in 70% ethanol for light microscopy investigation. Mature anthers from the collected flowers were left to dry, carefully opened using sharp needles, and sputtered onto glass slides. The pollen grains (25) were examined by an OPTIKA C-B 10 microscope and photographed using an OPTIKA am B 10 digital camera. Polar axis (P) and equatorial diameter (E) were measured; pollen size, shape, and aperture type were also assessed. For scanning electron microscope investigation, non-acetalized pollen grains were transferred onto a metallic stub using double-sided and coated with a thin layer of gold in a sputtering chamber, then scanned and photographed using a JEOL JSM-IT200 SEM for exine and aperture ornamentations. The terminology used for describing pollen grain morphology was followed as given by [30 ], Punt et al. [31 (link)], and Hesse et al. [32 ].
4
SEM Imaging of Organoids
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For scanning electron microscopy (SEM) organoids were fixed for 2 h at room temperature in 2.5 % glutaraldehyde (Sigma, Germany) in 0.1 M cacodylate buffer at pH 7.4 (Sciences Services, Germany), subsequently washed twice in 0.1 M cacodylate buffer at pH 7.4 and dehydrated stepwise in a graded ethanol series. Specimens were critical point dried in 100 % ethanol (CPD 300, Leica, Austria) and afterwards glued onto conductive carbon pads (Leit-Tabs, Plano, Germany) mounted on aluminum stubs (Plano, Germany) and were sputter-coated with a 15 nm gold layer (ACD600, Leica, Austria). SEM images were acquired with a JEOL JSM-IT200 SEM (JEOL, Japan) at 15 kV with a secondary electron detector.
5
Thermo-Mechanical Characterization of RPUF
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The RPUF’s thermo-mechanical properties were evaluated at
three independent replicates. The thermal conductivity (λ) was
assessed using a FOX 200 heat flow meter (Laser-Comp, Wakefield, MA)
by ASTM C518, with samples sized at 150 × 150 × 20 mm. The
apparent densities of the RPUF specimens were measured by following
the guidelines of ASTM D1622. The compressive strengths (σ10%)
of the RPUF samples were determined by utilizing a Universal Testing
Machine Shimadzu AGS-X Series (Shimadzu Corp., Kyoto, Japan) as per
ASTM D1621. Additionally, the characteristic thermal transitions in
the RPUF samples were analyzed via DSC using a PerkinElmer DSC 4000
(PerkinElmer, Waltham, MA) at a heating rate of 10 °C/min, employing
samples with a weight ranging from 5 to 10 mg. The TGA was conducted
using a Shimadzu DTG 60H (Shimadzu Corp., Kyoto, Japan) under a nitrogen
(N2) atmosphere, at a heating rate of 10 °C/per minute,
spanning the range from 45 to 800 °C, with samples weighing between
5 to 10 mg. The cellular structures of the RPUF samples were scrutinized
via SEM using a JEOL JSM-IT200 SEM (JEOL, Ltd., Tokyo, Japan). The
cell size distribution was analyzed using ImageJ software according
to the assessment of each cell’s area.51 (link) The average diameter for each cell is determined from the calculated
area of each cell, treating each area as that of a perfect circular
form.51 (link)−53 (link)
three independent replicates. The thermal conductivity (λ) was
assessed using a FOX 200 heat flow meter (Laser-Comp, Wakefield, MA)
by ASTM C518, with samples sized at 150 × 150 × 20 mm. The
apparent densities of the RPUF specimens were measured by following
the guidelines of ASTM D1622. The compressive strengths (σ10%)
of the RPUF samples were determined by utilizing a Universal Testing
Machine Shimadzu AGS-X Series (Shimadzu Corp., Kyoto, Japan) as per
ASTM D1621. Additionally, the characteristic thermal transitions in
the RPUF samples were analyzed via DSC using a PerkinElmer DSC 4000
(PerkinElmer, Waltham, MA) at a heating rate of 10 °C/min, employing
samples with a weight ranging from 5 to 10 mg. The TGA was conducted
using a Shimadzu DTG 60H (Shimadzu Corp., Kyoto, Japan) under a nitrogen
(N2) atmosphere, at a heating rate of 10 °C/per minute,
spanning the range from 45 to 800 °C, with samples weighing between
5 to 10 mg. The cellular structures of the RPUF samples were scrutinized
via SEM using a JEOL JSM-IT200 SEM (JEOL, Ltd., Tokyo, Japan). The
cell size distribution was analyzed using ImageJ software according
to the assessment of each cell’s area.51 (link) The average diameter for each cell is determined from the calculated
area of each cell, treating each area as that of a perfect circular
form.51 (link)−53 (link)
6
Microparticle Characterization by SEM
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Microparticle size, shape, surface morphology, and apparent contact angle were evaluated using scanning electron microscopy (SEM). The microparticles were coated with an Au-Pd mixture using a sputter coater unit (SC7620, Quorum, East Sussex, UK). Microparticle size and surface morphology were assessed using a JEOL JSM-IT200 SEM (JEOL, Tokyo, Japan) operated at an acceleration voltage of 5 kV. The microparticle size was measured using the SEM software (JSM-IT200 Operation software) roller. The apparent contact angle between the microparticles and the surface was determined using the SEM’s angle measurement function. The measurements were conducted on 10 randomly selected particles, and the average and standard deviations were reported.
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