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3 protocols using dextran sulphate sodium salt

1

Synthesis and Characterization of PLGA-based Biomaterials

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Poly(DL-lactide-co-glycolide) 50:50 (PLGA, Mw 45 kDa) was obtained from Boehringer Ingelheim (Belgium); oligo-(l)-lactide (OLL, Mw 5 kDa), oligo-(D)-lactide (OLD, Mw 5 kDa) were synthesized from the respective lactic acid isomers as described earlier [14 (link)]; Dextran sulphate sodium salt (DS) (Mw~9–15 kDa), Poly-l-arginine hydrochloride (PAr, Mw~15–70 kDa), Polyallylamine hydrochloride (PAH, Mw~58 kDa), Poly(sodium 4-styrenesulfonate) (PSS) (Mw~70 kDa), Poly vinyl alcohol (PVA, Mw 70 kDa), Fluorescein isothiocyanate (FITC), Rhodamine B isothiocyanate (RBITC), Resazurin sodium salt, Crystal Violet, EDTA, zimozan were all obtained from Sigma-Aldrich (Saint Louis, MO, USA); Phosphate buffered Saline (PBS), DMEM/F12, trypsin, penicillin and streptomycin were purchased from PanEco (Moscow, Russia); Fetal calf serum (FCS) was obtained from Invitrogen (Carlsbad, CA, USA); Chloroform was obtained from Chimmed (Moscow, Russia); DMSO was obtained from Panreac (Castellar del Vallès, Spain).
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2

Cellular Uptake and Membrane Effects

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Dextran sulphate sodium salt (DS, molecular weight ≈ 10 kDa, #D4911) and poly-L-arginine hydrochloride (PArg, MW ≈ 70 kDa, #P3892), calcium chloride (CaCl2, #223506), sodium carbonate (Na2CO3, #S7795), ethylenediaminetetraacetic acid disodium salt dihydrate (Na2EDTA 2H2O, #E5134), amantadine-HCl (#A1260), genistein(#G6649), amiloride-HCl(#A7410), NaN3 (#199931), cytochalasin D (#C2618),and Hoechst 33,342 (#14533) were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). All chemicals were used as received. Ultrapure water with a resistance greater than 18.2 MΩ cm−1 was used for all experiments. Syto 9 (#S34854) and propidium iodide (#BMS500PI), JC-1 (#T3168) and fluorescein isothiocyanate (FITC)-phalloidin (#F432) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).
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3

Fabrication of IgG-Fab Nanoparticles

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Human IgG-Fab fragment was purchased from Athens Research & Technology (Athens, GA) and used after purification by dialysis. Dextran sulphate sodium salt (molecular weight 5000Da), Poly (dl-lactide-co-glycolide) (PLGA 85:15, molecular weight of 50000-75000 Da), taurocholic acid and SDS were procured from Sigma Aldrich (St. Louis, MO). NuPAGE® gels were purchased from Invitrogen™ Life Technologies (Carlsbad, CA). Precision Plus Protein™ Kaleidoscope™ Standards was purchased from BIO-RAD (Hercules, CA). Bicinchoninic acid (BCA) protein assay and micro-BCA protein assay kits were purchased from Fisher Scientific (Pittsburgh, PA). All the solvents and other reagents of analytical grade were purchased from Fisher Scientific and used as received without any further purification. Double distilled water (DDW) was used throughout the study.
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