Automated glycohemoglobin analyser hlc 723g8

Fasting blood samples were obtained from each patient after 12-h fasting once upon admission. Blood samples were collected into EDTA-containing tube. After centrifugation at 3000 rpm for 10 min at 4 °C, plasma was collected and stored at − 80 °C. Plasma concentrations of total cholesterol (TC), TG, LDL-C and HDL-C were measured by automatic biochemistry analyzer (Hitachi 7150, Tokyo, Japan) in an enzymatic assay. Non-high density lipoprotein cholesterol (Non-HDL-C) was calculated as TC minus HDL-C. Plasma levels of sdLDL were measured by an automated homogeneous assay (DENKA SEIKEN CO., LTD, Tokyo, Japan) [19 (link)]. The measured sdLDL was with calibration range of 0.0–100 mg/dL. The concentrations of glucose were measured by enzymatic hexokinase method. HbA1c was measured using Tosoh Automated Glycohemoglobin Analyser (HLC-723G8, Tokyo, Japan).

Laboratory data were obtained from each patient with their venous blood samples taken after 12 h overnight at the initial entry in hospital, and all samples were stored in a − 80 °C refrigerator until test. The lipid profiles were measured by automatic biochemistry analyzer (Hitachi 7150, Tokyo, Japan) followed the methods of our previous study [23 (link)]. Detailly, the low-density lipoprotein cholesterol (LDL-C) concentrations were measured by selective solublilization method (low-density lipid cholesterol test kit; Kyowa Medex, Tokyo). High-density lipoprotein cholesterol (HDL-C) concentrations were analyzed using a homogeneous method (Determiner L HDL; Kyowa Medex, Tokyo). Total cholesterol, Triglyceride, Apolipoprotein A1 (ApoA1), and ApoB were measured by automatic biochemistry analyzer (Hitachi 7150, Tokyo, Japan) in an enzymatic assay. The fast glucose concentrations were measured by enzymatic hexokinase method. HbA1c was measured using Tosoh Automated Glycohemoglobin Analyser (HLC-723G8, Tokyo, Japan).

Blood samples of all patients were collected from cubital vein after fasting for at least 12 h upon admission and stored at − 80 °C until analysis. Concentrations of total cholesterol (TC), TG, LDL-C, high density lipoprotein cholesterol (HDL-C) and apolipoprotein B (apoB) were directly measured using an automatic biochemistry analyzer (Hitachi 7150, Tokyo, Japan). The non-HDL-C value was calculated as TC minus HDL-C. The concentrations of glucose were measured by enzymatic hexokinase method, while HbA1c was measured using Tosoh Automated Glycohemoglobin Analyser (HLC-723G8, Tokyo, Japan). Lipoprotein (a) [Lp(a)] levels were assayed by an immunoturbidimetry method as previously described [22 (link)]. Directly measured RC (MRC) was obtained using a two-step automated assay developed by Denka Seiken (Tokyo, Japan), measuring the cholesterol content in CR, VLDL, and IDL specifically, with the aid of enzymes and surfactants. The cholesterol in other lipoproteins was removed in the first step, and then the cholesterol in the remaining remnant lipoprotein particles were determined (for further details see Additional file 1: Methods) [10 (link), 12 (link)]. Inter-assay coefficients of variation for the RC assays were 4.8%. Calculated RC (CRC) was defined as TC minus LDL-C minus HDL-C [7 (link)].