For this study, 8- to 9-week-old male BALB/c (H-2d) mice and C57BL/6 (H-2b) mice were purchased from Orient Bio Inc. (Seongnam, Korea) and maintained according to the ethical guidelines of our institution.
The PE-Cy7-conjugated anti-mouse CD8, PerCP-Cy5.5-conjugated anti-mouse CD11c, PE-conjugated anti-mouse CD85k, FITC-conjugated anti-mouse CD44, PerCP-Cy5.5-conjugated anti-mouse FOXP3 antibodies, and the Fixation/Permeabilization kit were purchased from eBioscience (San Diego, CA, USA). APC-Cy7-conjugated anti-mouse CD4 antibodies were purchased from Biolegend (San Diego, CA, USA). TM, DX, and red blood cell lysis buffer, and Histopaque 1.083 were purchased from Sigma-Aldrich (St Louis, MO, USA). Mouse IL-6 enzyme-linked immunosorbent assay (ELISA) kit was purchased from BD Bioscience (San Jose, CA, USA).
The PE-Cy7-conjugated anti-mouse CD8, PerCP-Cy5.5-conjugated anti-mouse CD11c, PE-conjugated anti-mouse CD85k, FITC-conjugated anti-mouse CD44, PerCP-Cy5.5-conjugated anti-mouse FOXP3 antibodies, and the Fixation/Permeabilization kit were purchased from eBioscience (San Diego, CA, USA). APC-Cy7-conjugated anti-mouse CD4 antibodies were purchased from Biolegend (San Diego, CA, USA). TM, DX, and red blood cell lysis buffer, and Histopaque 1.083 were purchased from Sigma-Aldrich (St Louis, MO, USA). Mouse IL-6 enzyme-linked immunosorbent assay (ELISA) kit was purchased from BD Bioscience (San Jose, CA, USA).